Peripherin Antibody, clone 8G2 | EMD Millipore MAB1527 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Peripherin Antibody, clone 8G2

catalog :

MAB1527

quantity :

300 μL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8G2

The same clone is also sold as:

Novus Biologicals: NB300-138-100uL
Santa Cruz Biotechnology: sc-58834
MilliporeSigma: P5117
LifeSpan Biosciences: LS-C26029, LS-C204574, LS-C2838
Invitrogen: MA3-16724

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 28

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:500; loading ...; fig 3i	Mishra P, Boutej H, Soucy G, Bareil C, Kumar S, Picher Martel V, et al. Transmission of ALS pathogenesis by the cerebrospinal fluid. Acta Neuropathol Commun. 2020;8:65 pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; tbl 1	Pasquini J, Barrantes F, QuintÃ¡ H. Normal development of spinal axons in early embryo stages and posterior locomotor function is independent of GAL-1. J Comp Neurol. 2017;525:2861-2875 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 3b	Vyas P, Wu J, Zimmerman A, Fuchs P, Glowatzki E. Tyrosine Hydroxylase Expression in Type II Cochlear Afferents in Mice. J Assoc Res Otolaryngol. 2017;18:139-151 pubmed publisher
immunohistochemistry - frozen section; human	Dori A, Lopate G, Keeling R, Pestronk A. Myovascular innervation: axon loss in small-fiber neuropathies. Muscle Nerve. 2015;51:514-21 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:4000	Flores Otero J, Davis R. Synaptic proteins are tonotopically graded in postnatal and adult type I and type II spiral ganglion neurons. J Comp Neurol. 2011;519:1455-75 pubmed publisher
immunohistochemistry; mouse; 1:250	Hubert T, Bourane S, Venteo S, Mechaly I, Puech S, Valmier J, et al. Fibroblast growth factor homologous factor 1 (FHF1) is expressed in a subpopulation of calcitonin gene-related peptide-positive nociceptive neurons in the murine dorsal root ganglia. J Comp Neurol. 2008;507:1588-601 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100	Tseng K, Chau Y, Yang M, Lu K, Chien C. Abnormal cellular translocation of alpha-internexin in spinal motor neurons of Dystonia musculorum mice. J Comp Neurol. 2008;507:1053-64 pubmed
	Gunewardene N, Crombie D, Dottori M, Nayagam B. Innervation of Cochlear Hair Cells by Human Induced Pluripotent Stem Cell-Derived Neurons In Vitro. Stem Cells Int. 2016;2016:1781202 pubmed publisher
	Motohashi T, Watanabe N, Nishioka M, Nakatake Y, Yulan P, Mochizuki H, et al. Gene array analysis of neural crest cells identifies transcription factors necessary for direct conversion of embryonic fibroblasts into neural crest cells. Biol Open. 2016;5:311-22 pubmed publisher
	Liu Q, Swistowski A, Zeng X. Human neural crest stem cells derived from human pluripotent stem cells. Methods Mol Biol. 2014;1210:79-90 pubmed publisher
	Liu W, Davis R. Calretinin and calbindin distribution patterns specify subpopulations of type I and type II spiral ganglion neurons in postnatal murine cochlea. J Comp Neurol. 2014;522:2299-318 pubmed publisher
	Huang Y, Chang C, Chen C, Yang C, Sun W. Distinct expression of Mas1-related G-protein-coupled receptor B4 in dorsal root and trigeminal ganglia--implications for altered behaviors in acid-sensing ion channel 3-deficient mice. J Mol Neurosci. 2013;51:820-34 pubmed publisher
	Yuan A, Sasaki T, Kumar A, Peterhoff C, Rao M, LIEM R, et al. Peripherin is a subunit of peripheral nerve neurofilaments: implications for differential vulnerability of CNS and peripheral nervous system axons. J Neurosci. 2012;32:8501-8 pubmed publisher
	Nishiya Y, Yokokawa S, Fukuda A, Yamagata T, Inayoshi A, Obinata M, et al. The generation of rat dorsal root ganglion cell lines to identify the target of KW-7158, a novel treatment for overactive bladder. Neurosci Res. 2011;71:278-88 pubmed publisher
	Nayagam B, Minter R. A comparison of in vitro treatments for directing stem cells toward a sensory neural fate. Am J Otolaryngol. 2012;33:37-46 pubmed publisher
	Mair N, Benetti C, Andratsch M, Leitner M, Constantin C, Camprubí Robles M, et al. Genetic evidence for involvement of neuronally expressed S1P? receptor in nociceptor sensitization and inflammatory pain. PLoS ONE. 2011;6:e17268 pubmed publisher
	Chamberlain J, Pittock S, Oprescu A, Dege C, Apiwattanakul M, Kryzer T, et al. Peripherin-IgG association with neurologic and endocrine autoimmunity. J Autoimmun. 2010;34:469-77 pubmed publisher
	Perrot R, Julien J. Real-time imaging reveals defects of fast axonal transport induced by disorganization of intermediate filaments. FASEB J. 2009;23:3213-25 pubmed publisher
	Maison S, Casanova E, Holstein G, Bettler B, Liberman M. Loss of GABAB receptors in cochlear neurons: threshold elevation suggests modulation of outer hair cell function by type II afferent fibers. J Assoc Res Otolaryngol. 2009;10:50-63 pubmed publisher
	Nishio T, Kawaguchi S, Fujiwara H. Emergence of highly neurofilament-immunoreactive zipper-like axon segments at the transection site in scalpel-cordotomized adult rats. Neuroscience. 2008;155:90-103 pubmed publisher
	Kovacic U, Zele T, Mars T, Sketelj J, Bajrovic F. Aging impairs collateral sprouting of nociceptive axons in the rat. Neurobiol Aging. 2010;31:339-50 pubmed publisher
	McLean J, Xiao S, Miyazaki K, Robertson J. A novel peripherin isoform generated by alternative translation is required for normal filament network formation. J Neurochem. 2008;104:1663-73 pubmed publisher
	Shi F, Corrales C, Liberman M, Edge A. BMP4 induction of sensory neurons from human embryonic stem cells and reinnervation of sensory epithelium. Eur J Neurosci. 2007;26:3016-23 pubmed
	Millecamps S, Gowing G, Corti O, Mallet J, Julien J. Conditional NF-L transgene expression in mice for in vivo analysis of turnover and transport rate of neurofilaments. J Neurosci. 2007;27:4947-56 pubmed
	Millecamps S, Robertson J, Lariviere R, Mallet J, Julien J. Defective axonal transport of neurofilament proteins in neurons overexpressing peripherin. J Neurochem. 2006;98:926-38 pubmed
	Staton P, Wilson A, Bountra C, Chessell I, Day N. Changes in dorsal root ganglion CGRP expression in a chronic inflammatory model of the rat knee joint: differential modulation by rofecoxib and paracetamol. Eur J Pain. 2007;11:283-9 pubmed
	Robertson J, Doroudchi M, Nguyen M, Durham H, Strong M, Shaw G, et al. A neurotoxic peripherin splice variant in a mouse model of ALS. J Cell Biol. 2003;160:939-49 pubmed
	Robertson J, Beaulieu J, Doroudchi M, Durham H, Julien J, Mushynski W. Apoptotic death of neurons exhibiting peripherin aggregates is mediated by the proinflammatory cytokine tumor necrosis factor-alpha. J Cell Biol. 2001;155:217-26 pubmed

product information

Catalog Number :

MAB1527

Subcategory :

Neuroscience

Product Name :

Anti-Peripherin Antibody, clone 8G2

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P41219

Host Name :

Mouse

Antigen :

Peripherin

Clone :

8G2

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-Peripherin Antibody, clone 8G2

Cross Reactivity :

Bovine;Human;Mouse;Pig;Rat

Background :

Peripherin is a ~57kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems. Subsequently it was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig's disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration.

Immunogen :

Electrophoretically pure trp-E-peripherin fusion protein [Dev. Brain Res. (1990). 57:235-248] containing all but the 4 N-terminal amino acids of rat peripherin. Fusion protein purified from bacterial inclusion bodies by DEAE-cellulose chromatography in 6M urea followed by preparative SDS-PAGE

Specificity :

Recognizes Peripherin. Strong staining on rat, mouse, human, pig and cow peripherin. Does not work on chicken or other more distantly related species, which may lack peripherin.

Package Size :

300 μL

Uses :

Immunocytochemistry;Immunohistochemistry;Western Blotting

Storage :

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.