Actin Antibody, clone C4, Alexa Fluor® 488 conjugated | EMD Millipore MAB1501X product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Actin Antibody, clone C4, Alexa Fluor® 488 conjugated

catalog :

MAB1501X

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

AF488

clone name :

C4

The same clone is also sold as:

Abcam: ab14128
OriGene: TA309077
Abnova: MAB8172
MyBioSource: MBS500031
LifeSpan Biosciences: LS-C194931

reactivity :

human, chicken

application :

western blot, immunocytochemistry, flow cytometry

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 3	Tagscherer K, Fassl A, Sinkovic T, Richter J, Schecher S, Macher Goeppinger S, et al. MicroRNA-210 induces apoptosis in colorectal cancer via induction of reactive oxygen. Cancer Cell Int. 2016;16:42 pubmed publisher

product information

Catalog Number :

MAB1501X

Subcategory :

Cell Structure

Product Name :

Anti-Actin Antibody, clone C4, Alexa Fluor® 488 conjugated

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P68133;P62736;P60709;P63261;P63267;P68032

Host Name :

Mouse

Antigen :

Actin

Clone :

Conjugate :

AlexaFluor®488

Isotype :

IgG2bκ

Product Description :

Anti-Actin Antibody, clone C4, Alexa Fluor® 488 conjugated

Cross Reactivity :

Human;Chicken

Background :

Actin is an abundant cytoskeletal protein found in all cells (True, 1990). The protein's 42 kD peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction (True, 1990). In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis, and phagocytosis (True, 1990). Distribution of the six known isoforms of actin - four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific (Otey, 1986; Lessard, 1988).

ALT Names :

MAB1501;MAB1501R

Immunogen :

Purified chicken gizzard actin (Lessard, 1988).

Specificity :

MAB1501X is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). The epitope recognized by the antibody appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70. Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts (Lessard, 1983). Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).

Package Size :

100 µg

Uses :

Immunofluorescence

Storage :

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.