This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Actin Antibody, clone C4
catalog :
MAB1501
quantity :
100 µL
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
C4

The same clone is also sold as:
reactivity :
marine lamprey, Campylobacter jejuni, redtail notho, root, hamsters, African green monkey, human, mouse, rat, pigs , fruit fly , domestic rabbit
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation
citations: 1116
Published Application/Species/Sample/DilutionReference
  • western blot knockout validation; mouse; 1:4000; loading ...; fig 3a
Liu C, Stevens S, Teliska L, Stankewich M, Mohler P, Hund T, et al. Nodal β spectrins are required to maintain Na+ channel clustering and axon integrity. elife. 2020;9: pubmed publisher
  • western blot; human; 1:1000; loading ...; fig s2a
Viol L, Hata S, Pastor Peidro A, Neuner A, Murke F, Wuchter P, et al. Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis. J Cell Biol. 2020;219: pubmed publisher
  • western blot; human; loading ...; fig 1b
Zoi I, Karamouzis M, Xingi E, Sarantis P, Thomaidou D, Lembessis P, et al. Combining RANK/RANKL and ERBB-2 targeting as a novel strategy in ERBB-2-positive breast carcinomas. Breast Cancer Res. 2019;21:132 pubmed publisher
  • western blot; human; 1:10,000; loading ...; fig 6s1b
Cho C, Wang Y, Smallwood P, Williams J, Nathans J. Dlg1 activates beta-catenin signaling to regulate retinal angiogenesis and the blood-retina and blood-brain barriers. elife. 2019;8: pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 5k
A M, Fung T, Kettenbach A, Chakrabarti R, Higgs H. A complex containing lysine-acetylated actin inhibits the formin INF2. Nat Cell Biol. 2019;21:592-602 pubmed publisher
  • western blot; human; 1:5000; loading ...; fig 3b
Tsai C, Tsai C, Yi J, Kao H, Huang Y, Wang C, et al. Activin A regulates the epidermal growth factor receptor promoter by activating the PI3K/SP1 pathway in oral squamous cell carcinoma cells. Sci Rep. 2019;9:5197 pubmed publisher
  • western blot; root; loading ...; fig 5b
Ashok A, Karmakar S, Chandel R, Ravikumar R, Dalal S, Kong Q, et al. Prion protein modulates iron transport in the anterior segment: Implications for ocular iron homeostasis and prion transmission. Exp Eye Res. 2018;175:1-13 pubmed publisher
  • immunoprecipitation; human; loading ...; fig 3a
  • western blot; human; loading ...; fig 1b
Vélez P, Ocaranza Sánchez R, López Otero D, Grigorian Shamagian L, Rosa I, Guitián E, et al. Alteration of platelet GPVI signaling in ST-elevation myocardial infarction patients demonstrated by a combination of proteomic, biochemical, and functional approaches. Sci Rep. 2016;6:39603 pubmed publisher
  • western blot; human; fig 2
Lee J, Chiang K, Feng T, Chen Y, Chuang S, Tsui K, et al. The Iron Chelator, Dp44mT, Effectively Inhibits Human Oral Squamous Cell Carcinoma Cell Growth in Vitro and in Vivo. Int J Mol Sci. 2016;17: pubmed publisher
  • western blot; rat; 1:10,000; fig 1
Pan B, Huang X, Deng C. Chronic administration of aripiprazole activates GSK3β-dependent signalling pathways, and up-regulates GABAA receptor expression and CREB1 activity in rats. Sci Rep. 2016;6:30040 pubmed publisher
  • western blot; human; fig 2
Huang C, Lee C, Lin H, Chang J. Cathepsin S attenuates endosomal EGFR signalling: A mechanical rationale for the combination of cathepsin S and EGFR tyrosine kinase inhibitors. Sci Rep. 2016;6:29256 pubmed publisher
  • western blot; human; 1:10,000; fig 4
Tuncbag N, Milani P, Pokorny J, Johnson H, Sio T, Dalin S, et al. Network Modeling Identifies Patient-specific Pathways in Glioblastoma. Sci Rep. 2016;6:28668 pubmed publisher
  • western blot; human; 1:10,000; fig 4
Lange S, Gehmlich K, Lun A, Blondelle J, Hooper C, Dalton N, et al. MLP and CARP are linked to chronic PKC? signalling in dilated cardiomyopathy. Nat Commun. 2016;7:12120 pubmed publisher
  • western blot; mouse; 1:10,000; fig 2
Baksi S, Tripathi A, Singh N. Alpha-synuclein modulates retinal iron homeostasis by facilitating the uptake of transferrin-bound iron: Implications for visual manifestations of Parkinson's disease. Free Radic Biol Med. 2016;97:292-306 pubmed publisher
  • western blot; human; fig 4
Tsai K, Leung C, Lo Y, Chen T, Chan W, Yu S, et al. Arm Selection Preference of MicroRNA-193a Varies in Breast Cancer. Sci Rep. 2016;6:28176 pubmed publisher
  • western blot; Campylobacter jejuni; 1:2000; fig 4
Faber E, Gripp E, Maurischat S, Kaspers B, Tedin K, Menz S, et al. Novel Immunomodulatory Flagellin-Like Protein FlaC in Campylobacter jejuni and Other Campylobacterales. mSphere. 2016;1: pubmed publisher
  • western blot; mouse; fig 1
Bosch C, Muhaisen A, Pujadas L, Soriano E, MARTINEZ A. Reelin Exerts Structural, Biochemical and Transcriptional Regulation Over Presynaptic and Postsynaptic Elements in the Adult Hippocampus. Front Cell Neurosci. 2016;10:138 pubmed publisher
  • western blot; human; fig 3
Tagscherer K, Fassl A, Sinkovic T, Richter J, Schecher S, Macher Goeppinger S, et al. MicroRNA-210 induces apoptosis in colorectal cancer via induction of reactive oxygen. Cancer Cell Int. 2016;16:42 pubmed publisher
  • western blot; human; fig 1
Gilson T, Blanchette P, Ballmann M, Papp T, Pénzes J, Benkő M, et al. Using the E4orf6-Based E3 Ubiquitin Ligase as a Tool To Analyze the Evolution of Adenoviruses. J Virol. 2016;90:7350-7367 pubmed publisher
  • western blot; human; fig 3
Lin K, Cheng S, Tsai S, Tsai J, Lin C, Cheung C. Delivery of a survivin promoter-driven antisense survivin-expressing plasmid DNA as a cancer therapeutic: a proof-of-concept study. Onco Targets Ther. 2016;9:2601-13 pubmed publisher
  • western blot; human; fig 7
Hsu C, Hsu C, Hsueh C, Wang C, Wu Y, Wu C, et al. Identification and Characterization of Potential Biomarkers by Quantitative Tissue Proteomics of Primary Lung Adenocarcinoma. Mol Cell Proteomics. 2016;15:2396-410 pubmed publisher
  • western blot; human; 1:4000; fig 9
Rosiak K, Smolarz M, Stec W, Peciak J, Grzela D, Winiecka Klimek M, et al. IDH1R132H in Neural Stem Cells: Differentiation Impaired by Increased Apoptosis. PLoS ONE. 2016;11:e0154726 pubmed publisher
  • western blot; rat; fig 1
  • western blot; mouse; fig 1
Kim S, Choi K, Cho S, Yun S, Jeon J, Koh Y, et al. Fisetin stimulates autophagic degradation of phosphorylated tau via the activation of TFEB and Nrf2 transcription factors. Sci Rep. 2016;6:24933 pubmed publisher
  • western blot; mouse; fig 2
Novaira H, Graceli J, Capellino S, Schoeffield A, Hoffman G, Wolfe A, et al. Development and Characterization of Novel Rat Anti-mER? Sera. Endocrinology. 2016;157:2844-52 pubmed publisher
  • western blot; mouse; fig 6
  • western blot; human; fig 5
Kumar A, Chalamalasetty R, Kennedy M, Thomas S, Inala S, Garriock R, et al. Zfp703 Is a Wnt/?-Catenin Feedback Suppressor Targeting the ?-Catenin/Tcf1 Complex. Mol Cell Biol. 2016;36:1793-802 pubmed publisher
  • western blot; human; fig 6
  • western blot; mouse; fig 1
Nagashima H, Okuyama Y, Hayashi T, Ishii N, So T. TNFR-Associated Factors 2 and 5 Differentially Regulate the Instructive IL-6 Receptor Signaling Required for Th17 Development. J Immunol. 2016;196:4082-9 pubmed publisher
  • western blot; human; fig 2
Nivon M, Fort L, Muller P, Richet E, Simon S, Guey B, et al. NF?B is a central regulator of protein quality control in response to protein aggregation stresses via autophagy modulation. Mol Biol Cell. 2016;27:1712-27 pubmed publisher
  • western blot; human; 1:2000; fig 4
Carrió E, Magli A, Muñoz M, Peinado M, Perlingeiro R, Suelves M. Muscle cell identity requires Pax7-mediated lineage-specific DNA demethylation. BMC Biol. 2016;14:30 pubmed publisher
  • western blot; human; fig 1
Lee J, Kuo C, Tsai S, Cheng S, Chen S, Chan H, et al. Inhibition of HDAC3- and HDAC6-Promoted Survivin Expression Plays an Important Role in SAHA-Induced Autophagy and Viability Reduction in Breast Cancer Cells. Front Pharmacol. 2016;7:81 pubmed publisher
  • western blot; rat; 1:10,000; fig 2
Pan B, Huang X, Deng C. Aripiprazole and Haloperidol Activate GSK3?-Dependent Signalling Pathway Differentially in Various Brain Regions of Rats. Int J Mol Sci. 2016;17:459 pubmed publisher
  • western blot; human; fig 1
Braley A, Kwak T, Jules J, Harja E, Landgraf R, Hudson B. Regulation of Receptor for Advanced Glycation End Products (RAGE) Ectodomain Shedding and Its Role in Cell Function. J Biol Chem. 2016;291:12057-73 pubmed publisher
  • western blot; human; fig 3
Margaryan N, Gilgur A, Seftor E, Purnell C, Arva N, Gosain A, et al. Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi. Int J Mol Sci. 2016;17:418 pubmed publisher
  • western blot; human; fig 1
Ruf M, Moch H, Schraml P. PD-L1 expression is regulated by hypoxia inducible factor in clear cell renal cell carcinoma. Int J Cancer. 2016;139:396-403 pubmed publisher
  • western blot; mouse; fig 2
Barroso González J, Auclair S, Luan S, Thomas L, Atkins K, Aslan J, et al. PACS-2 mediates the ATM and NF-κB-dependent induction of anti-apoptotic Bcl-xL in response to DNA damage. Cell Death Differ. 2016;23:1448-57 pubmed publisher
  • western blot; human; 1:1000; fig 1
Keuss M, Thomas Y, Mcarthur R, Wood N, Knebel A, Kurz T. Characterization of the mammalian family of DCN-type NEDD8 E3 ligases. J Cell Sci. 2016;129:1441-54 pubmed publisher
  • western blot; mouse; fig 3e
Stine R, Shapira S, Lim H, Ishibashi J, Harms M, Won K, et al. EBF2 promotes the recruitment of beige adipocytes in white adipose tissue. Mol Metab. 2016;5:57-65 pubmed publisher
  • western blot; mouse; fig 4
Tai D, Liu Y, Hsu W, Ma Y, Cheng S, Liu S, et al. MeCP2 SUMOylation rescues Mecp2-mutant-induced behavioural deficits in a mouse model of Rett syndrome. Nat Commun. 2016;7:10552 pubmed publisher
  • western blot; mouse; 1:5000; fig 3
Lyons M, Chen L, Deng J, Finn C, Pfenning A, Sabhlok A, et al. The transcription factor calcium-response factor limits NMDA receptor-dependent transcription in the developing brain. J Neurochem. 2016;137:164-76 pubmed publisher
  • western blot; human; 1:20,000; fig 4b
Walter D, Hoffmann S, Komseli E, Rappsilber J, Gorgoulis V, Sørensen C. SCF(Cyclin F)-dependent degradation of CDC6 suppresses DNA re-replication. Nat Commun. 2016;7:10530 pubmed publisher
  • western blot; hamsters; 1:5000; fig 5
  • western blot; rat; 1:5000; fig 4
Krishnan B. Amygdala-Hippocampal Phospholipase D (PLD) Signaling As Novel Mechanism of Cocaine-Environment Maladaptive Conditioned Responses. Int J Neuropsychopharmacol. 2016;: pubmed publisher
  • western blot; human; fig 1
Kuo C, Li X, Stark J, Shih H, Ann D. RNF4 regulates DNA double-strand break repair in a cell cycle-dependent manner. Cell Cycle. 2016;15:787-98 pubmed publisher
  • western blot; human; fig 3
Zhang Q, Wan M, Shi J, Horita D, Miller L, Kute T, et al. Yin Yang 1 promotes mTORC2-mediated AKT phosphorylation. J Mol Cell Biol. 2016;8:232-43 pubmed publisher
  • western blot; mouse; fig 2
Kitayama M, Mizutani K, Maruoka M, Mandai K, Sakakibara S, Ueda Y, et al. A Novel Nectin-mediated Cell Adhesion Apparatus That Is Implicated in Prolactin Receptor Signaling for Mammary Gland Development. J Biol Chem. 2016;291:5817-31 pubmed publisher
  • western blot; mouse; 1:10,000; fig 2
Eckharter C, Junker N, Winter L, Fischer I, Fogli B, Kistner S, et al. Schwann Cell Expressed Nogo-B Modulates Axonal Branching of Adult Sensory Neurons Through the Nogo-B Receptor NgBR. Front Cell Neurosci. 2015;9:454 pubmed publisher
  • western blot; mouse; fig 5a
Dinh C, Szabo A, Yu Y, Camer D, Wang H, Huang X. Bardoxolone Methyl Prevents Mesenteric Fat Deposition and Inflammation in High-Fat Diet Mice. ScientificWorldJournal. 2015;2015:549352 pubmed publisher
  • western blot; human; fig 2
Ittig S, Schmutz C, Kasper C, Amstutz M, Schmidt A, Sauteur L, et al. A bacterial type III secretion-based protein delivery tool for broad applications in cell biology. J Cell Biol. 2015;211:913-31 pubmed publisher
  • western blot; human
Thorslund T, Ripplinger A, Hoffmann S, Wild T, Uckelmann M, Villumsen B, et al. Histone H1 couples initiation and amplification of ubiquitin signalling after DNA damage. Nature. 2015;527:389-93 pubmed publisher
  • western blot; human
Strizzi L, Sandomenico A, Margaryan N, Focà A, Sanguigno L, Bodenstine T, et al. Effects of a novel Nodal-targeting monoclonal antibody in melanoma. Oncotarget. 2015;6:34071-86 pubmed publisher
  • western blot; human; 1:1000; fig 2
Fidaleo M, Svetoni F, Volpe E, Miñana B, Caporossi D, Paronetto M. Genotoxic stress inhibits Ewing sarcoma cell growth by modulating alternative pre-mRNA processing of the RNA helicase DHX9. Oncotarget. 2015;6:31740-57 pubmed publisher
  • western blot; human; fig 1
Choulaki C, Papadaki G, Repa A, Kampouraki E, Kambas K, Ritis K, et al. Enhanced activity of NLRP3 inflammasome in peripheral blood cells of patients with active rheumatoid arthritis. Arthritis Res Ther. 2015;17:257 pubmed publisher
  • western blot; human
Sirohi K, Kumari A, Radha V, Swarup G. A Glaucoma-Associated Variant of Optineurin, M98K, Activates Tbk1 to Enhance Autophagosome Formation and Retinal Cell Death Dependent on Ser177 Phosphorylation of Optineurin. PLoS ONE. 2015;10:e0138289 pubmed publisher
  • western blot; fruit fly ; 1:10,000; fig s3i
Imai Y, Kobayashi Y, Inoshita T, Meng H, Arano T, Uemura K, et al. The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 2015;11:e1005503 pubmed publisher
  • western blot; human; fig 6f
Selleck E, Orchard R, Lassen K, Beatty W, Xavier R, Levine B, et al. A Noncanonical Autophagy Pathway Restricts Toxoplasma gondii Growth in a Strain-Specific Manner in IFN-γ-Activated Human Cells. MBio. 2015;6:e01157-15 pubmed publisher
  • western blot; mouse; 1:5000; fig 1
Choudhury S, Liu Y, Clark A, Pang I. Caspase-7: a critical mediator of optic nerve injury-induced retinal ganglion cell death. Mol Neurodegener. 2015;10:40 pubmed publisher
  • western blot; domestic rabbit; 1:5000; fig 2
Varela A, Piperi C, Sigala F, Agrogiannis G, Davos C, Andri M, et al. Elevated expression of mechanosensory polycystins in human carotid atherosclerotic plaques: association with p53 activation and disease severity. Sci Rep. 2015;5:13461 pubmed publisher
  • western blot; mouse; 1:1000; fig 6
Popugaeva E, Pchitskaya E, Speshilova A, Alexandrov S, Zhang H, Vlasova O, et al. STIM2 protects hippocampal mushroom spines from amyloid synaptotoxicity. Mol Neurodegener. 2015;10:37 pubmed publisher
  • western blot; mouse; 1:10,000; fig 2b
Levi M, Hasky N, Stemmer S, Shalgi R, Ben Aharon I. Anti-Müllerian Hormone Is a Marker for Chemotherapy-Induced Testicular Toxicity. Endocrinology. 2015;156:3818-27 pubmed publisher
  • western blot; human; fig 1
Nezich C, Wang C, Fogel A, Youle R. MiT/TFE transcription factors are activated during mitophagy downstream of Parkin and Atg5. J Cell Biol. 2015;210:435-50 pubmed publisher
  • western blot; rat; fig 5
Chesser A, Ganeshan V, Yang J, Johnson G. Epigallocatechin-3-gallate enhances clearance of phosphorylated tau in primary neurons. Nutr Neurosci. 2016;19:21-31 pubmed publisher
  • western blot; rat; 1:2000; fig 2e
Chen K, Tsai M, Wu C, Jou M, Wei I, Huang C. AMPA Receptor-mTOR Activation is Required for the Antidepressant-Like Effects of Sarcosine during the Forced Swim Test in Rats: Insertion of AMPA Receptor may Play a Role. Front Behav Neurosci. 2015;9:162 pubmed publisher
  • western blot; human; 1:10,000; fig 1
Panmanee J, Nopparat C, Chavanich N, Shukla M, Mukda S, Song W, et al. Melatonin regulates the transcription of βAPP-cleaving secretases mediated through melatonin receptors in human neuroblastoma SH-SY5Y cells. J Pineal Res. 2015;59:308-20 pubmed publisher
  • western blot; rat; 1:1000; fig 5
Liu K, Chuang S, Long C, Lee Y, Wang C, Lu M, et al. Ketamine-induced ulcerative cystitis and bladder apoptosis involve oxidative stress mediated by mitochondria and the endoplasmic reticulum. Am J Physiol Renal Physiol. 2015;309:F318-31 pubmed publisher
  • western blot; human; fig 5
Hu G, McQuiston T, Bernard A, Park Y, Qiu J, Vural A, et al. A conserved mechanism of TOR-dependent RCK-mediated mRNA degradation regulates autophagy. Nat Cell Biol. 2015;17:930-942 pubmed publisher
  • western blot; human; fig 2a
Gagnon J, Daou S, Zamorano N, Iannantuono N, Hammond Martel I, Mashtalir N, et al. Undetectable histone O-GlcNAcylation in mammalian cells. Epigenetics. 2015;10:677-91 pubmed publisher
  • western blot; human; fig 1
Yan R, Zhang Y, Cai D, Liu Y, Cuconati A, Guo H. Spinoculation Enhances HBV Infection in NTCP-Reconstituted Hepatocytes. PLoS ONE. 2015;10:e0129889 pubmed publisher
  • western blot; mouse
Dinh C, Szabo A, Yu Y, Camer D, Zhang Q, Wang H, et al. Bardoxolone Methyl Prevents Fat Deposition and Inflammation in Brown Adipose Tissue and Enhances Sympathetic Activity in Mice Fed a High-Fat Diet. Nutrients. 2015;7:4705-23 pubmed publisher
  • western blot; mouse; 1:700
Gulino R, Parenti R, Gulisano M. Novel Mechanisms of Spinal Cord Plasticity in a Mouse Model of Motoneuron Disease. Biomed Res Int. 2015;2015:654637 pubmed publisher
  • western blot; mouse; fig 1
Cui W, Sun M, Galeva N, Williams T, Azuma Y, Staudinger J. SUMOylation and Ubiquitylation Circuitry Controls Pregnane X Receptor Biology in Hepatocytes. Drug Metab Dispos. 2015;43:1316-25 pubmed publisher
  • western blot; human
Neo S, Itahana Y, Alagu J, Kitagawa M, Guo A, Lee S, et al. TRIM28 Is an E3 Ligase for ARF-Mediated NPM1/B23 SUMOylation That Represses Centrosome Amplification. Mol Cell Biol. 2015;35:2851-63 pubmed publisher
  • western blot; human; fig 4
O Callaghan K, Palagano E, Butini S, Campiani G, Williams D, Zisterer D, et al. Induction of apoptosis in oral squamous carcinoma cells by pyrrolo-1,5-benzoxazepines. Mol Med Rep. 2015;12:3748-3754 pubmed publisher
  • western blot; human; fig s7
Webb B, Forouhar F, Szu F, Seetharaman J, Tong L, Barber D. Structures of human phosphofructokinase-1 and atomic basis of cancer-associated mutations. Nature. 2015;523:111-4 pubmed publisher
  • western blot; rat
Calafate S, Buist A, Miskiewicz K, Vijayan V, Daneels G, De Strooper B, et al. Synaptic Contacts Enhance Cell-to-Cell Tau Pathology Propagation. Cell Rep. 2015;11:1176-83 pubmed publisher
  • immunocytochemistry; mouse; fig 6
Ketema M, Secades P, Kreft M, Nahidiazar L, Janssen H, Jalink K, et al. The rod domain is not essential for the function of plectin in maintaining tissue integrity. Mol Biol Cell. 2015;26:2402-17 pubmed publisher
  • western blot; human; tbl s3
Cheng H, Liang Y, Kuo Y, Chuu C, Lin C, Lee M, et al. Identification of thioridazine, an antipsychotic drug, as an antiglioblastoma and anticancer stem cell agent using public gene expression data. Cell Death Dis. 2015;6:e1753 pubmed publisher
  • western blot; human; fig 5
Goldstein M, Kastan M. Repair versus Checkpoint Functions of BRCA1 Are Differentially Regulated by Site of Chromatin Binding. Cancer Res. 2015;75:2699-707 pubmed publisher
  • western blot; human; 1:10,000; fig 2
Hung T, Li Y, Tseng C, Lan Y, Hsu S, Chen Y, et al. Knockdown of c-MET induced apoptosis in ABCB1-overexpressed multidrug-resistance cancer cell lines. Cancer Gene Ther. 2015;22:262-70 pubmed publisher
  • western blot; human; 1:10,000; fig 2
Alkhateeb A, Buckett P, Gardeck A, Kim J, Byrne S, Fraenkel P, et al. The small molecule ferristatin II induces hepatic hepcidin expression in vivo and in vitro. Am J Physiol Gastrointest Liver Physiol. 2015;308:G1019-26 pubmed publisher
  • western blot; mouse; 1:10,000; fig 2
Dilsizoglu Senol A, Tagliafierro L, Huguet L, Gorisse Hussonnois L, Chasseigneaux S, Allinquant B. PAT1 inversely regulates the surface Amyloid Precursor Protein level in mouse primary neurons. BMC Neurosci. 2015;16:10 pubmed publisher
  • western blot; human
Fukumoto M, Kurisu S, Yamada T, Takenawa T. α-Actinin-4 enhances colorectal cancer cell invasion by suppressing focal adhesion maturation. PLoS ONE. 2015;10:e0120616 pubmed publisher
  • western blot; human
Malchenko S, Sredni S, Hashimoto H, Kasai A, Nagayasu K, Xie J, et al. A mouse model of human primitive neuroectodermal tumors resulting from microenvironmentally-driven malignant transformation of orthotopically transplanted radial glial cells. PLoS ONE. 2015;10:e0121707 pubmed publisher
  • immunoprecipitation; human
  • western blot; human
Leslie P, Ke H, Zhang Y. The MDM2 RING domain and central acidic domain play distinct roles in MDM2 protein homodimerization and MDM2-MDMX protein heterodimerization. J Biol Chem. 2015;290:12941-50 pubmed publisher
  • western blot; human; fig 1
Wang D, Chadha G, Feygin A, Ivanov A. F-actin binding protein, anillin, regulates integrity of intercellular junctions in human epithelial cells. Cell Mol Life Sci. 2015;72:3185-3200 pubmed publisher
  • western blot; mouse; 1:20000
Leirós M, Alonso E, Rateb M, Houssen W, Ebel R, Jaspars M, et al. Gracilins: Spongionella-derived promising compounds for Alzheimer disease. Neuropharmacology. 2015;93:285-93 pubmed publisher
  • western blot; rat; 1:7500; tbl 1
Van Skike C, Diaz Granados J, Matthews D. Chronic intermittent ethanol exposure produces persistent anxiety in adolescent and adult rats. Alcohol Clin Exp Res. 2015;39:262-71 pubmed publisher
  • western blot; rat; 1:10000
Briz V, Zhu G, Wang Y, Liu Y, Avetisyan M, Bi X, et al. Activity-dependent rapid local RhoA synthesis is required for hippocampal synaptic plasticity. J Neurosci. 2015;35:2269-82 pubmed publisher
  • western blot; mouse; fig 4
Dinh C, Szabo A, Camer D, Yu Y, Wang H, Huang X. Bardoxolone methyl prevents fat deposition and inflammation in the visceral fat of mice fed a high-fat diet. Chem Biol Interact. 2015;229:1-8 pubmed publisher
  • western blot; human; fig 2
Bilal M, Zhang E, Dinkel B, Hardy D, Yankee T, Houtman J. GADS is required for TCR-mediated calcium influx and cytokine release, but not cellular adhesion, in human T cells. Cell Signal. 2015;27:841-50 pubmed publisher
  • western blot; human; fig 5
Huang P, Hung S, Pao C, Wang T. N-(1-pyrenyl) maleimide induces bak oligomerization and mitochondrial dysfunction in Jurkat Cells. Biomed Res Int. 2015;2015:798489 pubmed publisher
  • western blot; human; fig 1
Wong E, Wong S, Chan C, Lam E, Ho L, Lau C, et al. TP53-induced glycolysis and apoptosis regulator promotes proliferation and invasiveness of nasopharyngeal carcinoma cells. Oncol Lett. 2015;9:569-574 pubmed
  • western blot; human; 1:10000
Ninio Many L, Grossman H, Levi M, Zilber S, Tsarfaty I, Shomron N, et al. MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells. Oncoscience. 2014;1:250-261 pubmed
  • western blot; human; fig 1
Hsu S, Su W, Jeng K, Lai M. A host susceptibility gene, DR1, facilitates influenza A virus replication by suppressing host innate immunity and enhancing viral RNA replication. J Virol. 2015;89:3671-82 pubmed publisher
  • western blot; human; 1:500; fig 4
Naegelen I, Plançon S, Nicot N, Kaoma T, Muller A, Vallar L, et al. An essential role of syntaxin 3 protein for granule exocytosis and secretion of IL-1α, IL-1β, IL-12b, and CCL4 from differentiated HL-60 cells. J Leukoc Biol. 2015;97:557-71 pubmed publisher
  • western blot; human
Hasegawa H, Ishibashi K, Kubota S, Yamaguchi C, Yuki R, Nakajo H, et al. Cdk1-mediated phosphorylation of human ATF7 at Thr-51 and Thr-53 promotes cell-cycle progression into M phase. PLoS ONE. 2014;9:e116048 pubmed publisher
  • western blot; human; fig 3
Gomez Bougie P, Halliez M, Maïga S, Godon C, Kervoëlen C, Pellat Deceunynck C, et al. Curcumin induces cell death of the main molecular myeloma subtypes, particularly the poor prognosis subgroups. Cancer Biol Ther. 2015;16:60-5 pubmed publisher
  • western blot; human
Xue L, Furusawa Y, Okayasu R, Miura M, Cui X, Liu C, et al. The complexity of DNA double strand break is a crucial factor for activating ATR signaling pathway for G2/M checkpoint regulation regardless of ATM function. DNA Repair (Amst). 2015;25:72-83 pubmed publisher
  • western blot; mouse
Hoon J, Li H, Koh C. POPX2 phosphatase regulates cell polarity and centrosome placement. Cell Cycle. 2014;13:2459-68 pubmed publisher
  • western blot; mouse; 1:100000
Hsiao Y, Hung H, Chen S, Gean P. Social interaction rescues memory deficit in an animal model of Alzheimer's disease by increasing BDNF-dependent hippocampal neurogenesis. J Neurosci. 2014;34:16207-19 pubmed publisher
  • western blot; human; fig 3
Lew Q, Chu K, Chia Y, Soo B, Ho J, Ng C, et al. GCN5 inhibits XBP-1S-mediated transcription by antagonizing PCAF action. Oncotarget. 2015;6:271-87 pubmed
  • western blot; hamsters
Hall J, Sun J, Slade J, Kintner J, Bambino M, Whittimore J, et al. Host nectin-1 is required for efficient Chlamydia trachomatis serovar E development. Front Cell Infect Microbiol. 2014;4:158 pubmed publisher
  • western blot; human
Hung T, Hsu S, Cheng C, Choo K, Tseng C, Chen T, et al. Wnt5A regulates ABCB1 expression in multidrug-resistant cancer cells through activation of the non-canonical PKA/β-catenin pathway. Oncotarget. 2014;5:12273-90 pubmed
  • western blot; human; fig 5
Choi M, Nakamura T, Cho S, Han X, Holland E, Qu J, et al. Transnitrosylation from DJ-1 to PTEN attenuates neuronal cell death in parkinson's disease models. J Neurosci. 2014;34:15123-31 pubmed publisher
  • western blot; mouse; fig 7
Ho T, Zollinger D, Chang K, Xu M, Cooper E, Stankewich M, et al. A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier. Nat Neurosci. 2014;17:1664-72 pubmed publisher
  • western blot; mouse; fig 1
Chang K, Zollinger D, Susuki K, Sherman D, Makara M, Brophy P, et al. Glial ankyrins facilitate paranodal axoglial junction assembly. Nat Neurosci. 2014;17:1673-81 pubmed publisher
  • western blot; mouse; 1:10,000
Shiba Fukushima K, Inoshita T, Hattori N, Imai Y. Lysine 63-linked polyubiquitination is dispensable for Parkin-mediated mitophagy. J Biol Chem. 2014;289:33131-6 pubmed publisher
  • western blot; redtail notho; 1:10000
Wang X, Chang Q, Wang Y, Su F, Zhang S. Late-onset temperature reduction can retard the aging process in aged fish via a combined action of an anti-oxidant system and the insulin/insulin-like growth factor 1 signaling pathway. Rejuvenation Res. 2014;17:507-17 pubmed publisher
  • western blot; human; 1:10000
Wei H, Nickoloff J, Chen W, Liu H, Lo W, Chang Y, et al. FOXF1 mediates mesenchymal stem cell fusion-induced reprogramming of lung cancer cells. Oncotarget. 2014;5:9514-29 pubmed
  • western blot; mouse; fig 3
Sontag J, Wasek B, Taleski G, Smith J, Arning E, Sontag E, et al. Altered protein phosphatase 2A methylation and Tau phosphorylation in the young and aged brain of methylenetetrahydrofolate reductase (MTHFR) deficient mice. Front Aging Neurosci. 2014;6:214 pubmed publisher
  • western blot; rat; 1:2,000
Friedman L, Riemslagh F, Sullivan J, Mesias R, Williams F, Huntley G, et al. Cadherin-8 expression, synaptic localization, and molecular control of neuronal form in prefrontal corticostriatal circuits. J Comp Neurol. 2015;523:75-92 pubmed publisher
  • western blot; human
Gargalionis A, Korkolopoulou P, Farmaki E, Piperi C, Dalagiorgou G, Adamopoulos C, et al. Polycystin-1 and polycystin-2 are involved in the acquisition of aggressive phenotypes in colorectal cancer. Int J Cancer. 2015;136:1515-27 pubmed publisher
  • western blot; hamsters; 1:5000
Bergström J, Berg K, Rodríguez Piñeiro A, Stecher B, Johansson M, Hansson G. AGR2, an endoplasmic reticulum protein, is secreted into the gastrointestinal mucus. PLoS ONE. 2014;9:e104186 pubmed publisher
  • western blot; human
Dutta B, Yan R, Lim S, Tam J, Sze S. Quantitative profiling of chromatome dynamics reveals a novel role for HP1BP3 in hypoxia-induced oncogenesis. Mol Cell Proteomics. 2014;13:3236-49 pubmed publisher
  • western blot; fruit fly ; fig 3
Burman J, Itsara L, Kayser E, Suthammarak W, Wang A, Kaeberlein M, et al. A Drosophila model of mitochondrial disease caused by a complex I mutation that uncouples proton pumping from electron transfer. Dis Model Mech. 2014;7:1165-74 pubmed publisher
  • western blot; human
Legrand N, Araud T, Conne B, Kuijpers O, Jaquier Gubler P, Curran J. An AUG codon conserved for protein function rather than translational initiation: the story of the protein sElk1. PLoS ONE. 2014;9:e102890 pubmed publisher
  • western blot; human
Tavares C, Ferguson S, Giles D, Wang Q, Wellmann R, O Brien J, et al. The molecular mechanism of eukaryotic elongation factor 2 kinase activation. J Biol Chem. 2014;289:23901-16 pubmed publisher
  • western blot; human; 1:50,000; fig 1
Fernandez Vidal A, Guitton Sert L, Cadoret J, Drac M, Schwob E, Baldacci G, et al. A role for DNA polymerase ? in the timing of DNA replication. Nat Commun. 2014;5:4285 pubmed publisher
  • western blot; mouse
Wijayatunge R, Chen L, Cha Y, Zannas A, Frank C, West A. The histone lysine demethylase Kdm6b is required for activity-dependent preconditioning of hippocampal neuronal survival. Mol Cell Neurosci. 2014;61:187-200 pubmed publisher
  • western blot; human; 0.2 ug/ml; fig 1
Yamamoto J, Hagiwara Y, Chiba K, Isobe T, Narita T, Handa H, et al. DSIF and NELF interact with Integrator to specify the correct post-transcriptional fate of snRNA genes. Nat Commun. 2014;5:4263 pubmed publisher
  • western blot; rat; 1:40000
  • western blot; human; 1:40000
Rice M, Smith K, Roberts R, Perez Costas E, Melendez Ferro M. Assessment of cytochrome C oxidase dysfunction in the substantia nigra/ventral tegmental area in schizophrenia. PLoS ONE. 2014;9:e100054 pubmed publisher
  • western blot; rat
Kopaliani I, Martin M, Zatschler B, Bortlik K, Müller B, Deussen A. Cell-specific and endothelium-dependent regulations of matrix metalloproteinase-2 in rat aorta. Basic Res Cardiol. 2014;109:419 pubmed publisher
  • western blot; fruit fly ; 1:50000
Thomas R, Andrews L, Burman J, Lin W, Pallanck L. PINK1-Parkin pathway activity is regulated by degradation of PINK1 in the mitochondrial matrix. PLoS Genet. 2014;10:e1004279 pubmed publisher
  • western blot; human; 1:5,000
Li Y, Ehrhardt K, Zhang M, Bleris L. Assembly and validation of versatile transcription activator-like effector libraries. Sci Rep. 2014;4:4857 pubmed publisher
  • western blot; mouse
  • western blot; human
Chuderland D, Ben Ami I, Friedler S, Hasky N, Ninio Many L, Goldberg K, et al. Hormonal regulation of pigment epithelium-derived factor (PEDF) expression in the endometrium. Mol Cell Endocrinol. 2014;390:85-92 pubmed publisher
  • western blot; mouse; 1:4000
Nikitczuk J, Patil S, Matikainen Ankney B, Scarpa J, Shapiro M, Benson D, et al. N-cadherin regulates molecular organization of excitatory and inhibitory synaptic circuits in adult hippocampus in vivo. Hippocampus. 2014;24:943-962 pubmed publisher
  • western blot; human; 1:10000
Ishikawa K, Saiki S, Furuya N, Yamada D, Imamichi Y, Li Y, et al. P150glued-associated disorders are caused by activation of intrinsic apoptotic pathway. PLoS ONE. 2014;9:e94645 pubmed publisher
  • western blot; human; 1:5000
Chou C, Huang N, Jhuang S, Pan H, Peng N, Cheng J, et al. Ubiquitin-conjugating enzyme UBE2C is highly expressed in breast microcalcification lesions. PLoS ONE. 2014;9:e93934 pubmed publisher
  • western blot; mouse; 1:20000
Deng J, Wan Y, Wang X, Cohen S, Wetsel W, Greenberg M, et al. MeCP2 phosphorylation limits psychostimulant-induced behavioral and neuronal plasticity. J Neurosci. 2014;34:4519-27 pubmed publisher
  • western blot; human
Yeh Y, Chen C, Huang P, Hsu C, Wu C, Wang T. Proteomic analyses of genes regulated by heterogeneous nuclear ribonucleoproteins A/B in Jurkat cells. Proteomics. 2014;14:1357-66 pubmed publisher
  • western blot; human; fig 1i
Fukumoto Y, Morii M, Miura T, Kubota S, Ishibashi K, Honda T, et al. Src family kinases promote silencing of ATR-Chk1 signaling in termination of DNA damage checkpoint. J Biol Chem. 2014;289:12313-29 pubmed publisher
  • western blot; rat; 1:5000
Cheng C, Lin J, Su S, Tang N, Kao S, Hsieh C. Electroacupuncture-like stimulation at Baihui and Dazhui acupoints exerts neuroprotective effects through activation of the brain-derived neurotrophic factor-mediated MEK1/2/ERK1/2/p90RSK/bad signaling pathway in mild transient focal cerebral ischemi. BMC Complement Altern Med. 2014;14:92 pubmed publisher
  • western blot; mouse; 1:100000
Pujadas L, Rossi D, Andres R, Teixeira C, Serra Vidal B, Parcerisas A, et al. Reelin delays amyloid-beta fibril formation and rescues cognitive deficits in a model of Alzheimer's disease. Nat Commun. 2014;5:3443 pubmed publisher
  • western blot; mouse
Toyoshima D, Mandai K, Maruo T, Supriyanto I, Togashi H, Inoue T, et al. Afadin regulates puncta adherentia junction formation and presynaptic differentiation in hippocampal neurons. PLoS ONE. 2014;9:e89763 pubmed publisher
  • western blot; human
Maruyama Y, Sou Y, Kageyama S, Takahashi T, Ueno T, Tanaka K, et al. LC3B is indispensable for selective autophagy of p62 but not basal autophagy. Biochem Biophys Res Commun. 2014;446:309-15 pubmed publisher
  • western blot; human
Guezguez A, Paré F, Benoit Y, Basora N, Beaulieu J. Modulation of stemness in a human normal intestinal epithelial crypt cell line by activation of the WNT signaling pathway. Exp Cell Res. 2014;322:355-64 pubmed publisher
  • western blot; mouse; 1:500
Dorn C, Engelmann J, Saugspier M, Koch A, Hartmann A, Müller M, et al. Increased expression of c-Jun in nonalcoholic fatty liver disease. Lab Invest. 2014;94:394-408 pubmed publisher
  • western blot; human
Stanevich V, Zheng A, Guo F, Jiang L, Wlodarchak N, Xing Y. Mechanisms of the scaffold subunit in facilitating protein phosphatase 2A methylation. PLoS ONE. 2014;9:e86955 pubmed publisher
  • western blot; human
Traer E, Javidi Sharifi N, Agarwal A, Dunlap J, English I, Martinez J, et al. Ponatinib overcomes FGF2-mediated resistance in CML patients without kinase domain mutations. Blood. 2014;123:1516-24 pubmed publisher
  • western blot; mouse; 1:5000
Portugal G, Al Hasani R, Fakira A, Gonzalez Romero J, Melyan Z, McCall J, et al. Hippocampal long-term potentiation is disrupted during expression and extinction but is restored after reinstatement of morphine place preference. J Neurosci. 2014;34:527-38 pubmed publisher
  • western blot; mouse; 1:5000
Barras D, Lorusso G, Lhermitte B, Viertl D, Ruegg C, Widmann C. Fragment N2, a caspase-3-generated RasGAP fragment, inhibits breast cancer metastatic progression. Int J Cancer. 2014;135:242-7 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
  • western blot; mouse
Ushijima T, Okazaki K, Tsushima H, Iwamoto Y. CCAAT/enhancer-binding protein ? regulates the repression of type II collagen expression during the differentiation from proliferative to hypertrophic chondrocytes. J Biol Chem. 2014;289:2852-63 pubmed publisher
  • western blot; mouse
ElAli A, Theriault P, Prefontaine P, Rivest S. Mild chronic cerebral hypoperfusion induces neurovascular dysfunction, triggering peripheral beta-amyloid brain entry and aggregation. Acta Neuropathol Commun. 2013;1:75 pubmed publisher
  • western blot; rat; 1:3000000
Mizunoya W, Iwamoto Y, Shirouchi B, Sato M, Komiya Y, Razin F, et al. Dietary fat influences the expression of contractile and metabolic genes in rat skeletal muscle. PLoS ONE. 2013;8:e80152 pubmed publisher
  • western blot; rat; 1:3000000
Mizunoya W, Iwamoto Y, Sato Y, Tatsumi R, Ikeuchi Y. Cold exposure increases slow-type myosin heavy chain 1 (MyHC1) composition of soleus muscle in rats. Anim Sci J. 2014;85:293-304 pubmed publisher
  • western blot; human
Almenar Queralt A, Kim S, Benner C, Herrera C, Kang D, Garcia Bassets I, et al. Presenilins regulate neurotrypsin gene expression and neurotrypsin-dependent agrin cleavage via cyclic AMP response element-binding protein (CREB) modulation. J Biol Chem. 2013;288:35222-36 pubmed publisher
  • western blot; human
Bobinet M, Vignard V, Florenceau L, Lang F, Labarriere N, Moreau Aubry A. Overexpression of meloe gene in melanomas is controlled both by specific transcription factors and hypomethylation. PLoS ONE. 2013;8:e75421 pubmed publisher
  • western blot; human; 1:1000
Yu Y, Munger K. Human papillomavirus type 16 E7 oncoprotein inhibits the anaphase promoting complex/cyclosome activity by dysregulating EMI1 expression in mitosis. Virology. 2013;446:251-9 pubmed publisher
  • western blot; mouse; 1:10,000
Kr cher T, Malinovskaja K, J rgenson M, Aonurm Helm A, Zharkovskaya T, Kalda A, et al. Schizophrenia-like phenotype of polysialyltransferase ST8SIA2-deficient mice. Brain Struct Funct. 2015;220:71-83 pubmed publisher
  • western blot; African green monkey; 1:1000
  • western blot; mouse; 1:1000
Hall B, Wankhade U, Konkel J, Cherukuri K, Nagineni C, Flanders K, et al. Transforming growth factor-?3 (TGF-?3) knock-in ameliorates inflammation due to TGF-?1 deficiency while promoting glucose tolerance. J Biol Chem. 2013;288:32074-92 pubmed publisher
  • western blot; human; fig s1
Wittchen E, Nishimura E, McCloskey M, Wang H, Quilliam L, Chrzanowska Wodnicka M, et al. Rap1 GTPase activation and barrier enhancement in rpe inhibits choroidal neovascularization in vivo. PLoS ONE. 2013;8:e73070 pubmed publisher
  • western blot; mouse
Zhang X, Meyn M, Smithgall T. c-Yes tyrosine kinase is a potent suppressor of ES cell differentiation and antagonizes the actions of its closest phylogenetic relative, c-Src. ACS Chem Biol. 2014;9:139-46 pubmed publisher
  • western blot; rat
Belzil C, Neumayer G, Vassilev A, Yap K, Konishi H, Rivest S, et al. A Ca2+-dependent mechanism of neuronal survival mediated by the microtubule-associated protein p600. J Biol Chem. 2013;288:24452-64 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:500
Milman P, Woulfe J. Novel variant of neuronal intranuclear rodlet immunoreactive for 40 kDa huntingtin associated protein and ubiquitin in the mouse brain. J Comp Neurol. 2013;521:3832-46 pubmed publisher
  • western blot; human
Kubota S, Fukumoto Y, Aoyama K, Ishibashi K, Yuki R, Morinaga T, et al. Phosphorylation of KRAB-associated protein 1 (KAP1) at Tyr-449, Tyr-458, and Tyr-517 by nuclear tyrosine kinases inhibits the association of KAP1 and heterochromatin protein 1? (HP1?) with heterochromatin. J Biol Chem. 2013;288:17871-83 pubmed publisher
  • immunohistochemistry; rat; 1:5000
Sogn C, Puchades M, Gundersen V. Rare contacts between synapses and microglial processes containing high levels of Iba1 and actin--a postembedding immunogold study in the healthy rat brain. Eur J Neurosci. 2013;38:2030-40 pubmed publisher
  • western blot; mouse
  • western blot; human
Benoit Y, Witherspoon M, Laursen K, Guezguez A, Beauséjour M, Beaulieu J, et al. Pharmacological inhibition of polycomb repressive complex-2 activity induces apoptosis in human colon cancer stem cells. Exp Cell Res. 2013;319:1463-70 pubmed publisher
  • western blot; mouse; fig 1
Song W, Mondal P, Li Y, Lee S, Hussain M. Pancreatic ?-cell response to increased metabolic demand and to pharmacologic secretagogues requires EPAC2A. Diabetes. 2013;62:2796-807 pubmed publisher
  • western blot; mouse; 1:1,000
El Achkar T, McCracken R, Liu Y, Heitmeier M, Bourgeois S, Ryerse J, et al. Tamm-Horsfall protein translocates to the basolateral domain of thick ascending limbs, interstitium, and circulation during recovery from acute kidney injury. Am J Physiol Renal Physiol. 2013;304:F1066-75 pubmed publisher
  • western blot; human
Chen M, Huang C, Hsu S, Lin E, Ku C, Lin H, et al. Retinoic Acid Induces Apoptosis of Prostate Cancer DU145 Cells through Cdk5 Overactivation. Evid Based Complement Alternat Med. 2012;2012:580736 pubmed publisher
  • western blot; mouse; loading ...; fig 5a
Tomasek J, Haaksma C, Schwartz R, Howard E. Whole animal knockout of smooth muscle alpha-actin does not alter excisional wound healing or the fibroblast-to-myofibroblast transition. Wound Repair Regen. 2013;21:166-76 pubmed publisher
  • western blot; human
Alimova I, Birks D, Harris P, Knipstein J, Venkataraman S, Marquez V, et al. Inhibition of EZH2 suppresses self-renewal and induces radiation sensitivity in atypical rhabdoid teratoid tumor cells. Neuro Oncol. 2013;15:149-60 pubmed publisher
  • western blot; mouse; 1:5000
Chang Y, Lin L, Lou C, Chou C, Ch ang H. Bone marrow transplantation rescues intestinal mucosa after whole body radiation via paracrine mechanisms. Radiother Oncol. 2012;105:371-7 pubmed publisher
  • western blot; human; 1:20000
Chen Y, Wu K, Chen C. Methamphetamine reduces human influenza A virus replication. PLoS ONE. 2012;7:e48335 pubmed publisher
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Henke N, Albrecht P, Pfeiffer A, Toutzaris D, Zanger K, Methner A. Stromal interaction molecule 1 (STIM1) is involved in the regulation of mitochondrial shape and bioenergetics and plays a role in oxidative stress. J Biol Chem. 2012;287:42042-52 pubmed publisher
  • immunohistochemistry - paraffin section; mouse
  • western blot; mouse
Martin S, Fernandez Rojo M, Stanley A, Bastiani M, Okano S, Nixon S, et al. Caveolin-1 deficiency leads to increased susceptibility to cell death and fibrosis in white adipose tissue: characterization of a lipodystrophic model. PLoS ONE. 2012;7:e46242 pubmed publisher
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Fitzgerald A, Benz C, Clark A, Wordinger R. The effects of transforming growth factor-?2 on the expression of follistatin and activin A in normal and glaucomatous human trabecular meshwork cells and tissues. Invest Ophthalmol Vis Sci. 2012;53:7358-69 pubmed publisher
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Hirota T, Lee J, St John P, Sawa M, Iwaisako K, Noguchi T, et al. Identification of small molecule activators of cryptochrome. Science. 2012;337:1094-7 pubmed publisher
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Michaud M, Martins I, Sukkurwala A, Adjemian S, Ma Y, Pellegatti P, et al. Autophagy-dependent anticancer immune responses induced by chemotherapeutic agents in mice. Science. 2011;334:1573-7 pubmed publisher
  • western blot; human
Ní Chonghaile T, Sarosiek K, Vo T, Ryan J, Tammareddi A, Moore V, et al. Pretreatment mitochondrial priming correlates with clinical response to cytotoxic chemotherapy. Science. 2011;334:1129-33 pubmed publisher
  • western blot; human; fig 2
Su W, Chao T, Huang Y, Weng S, Jeng K, Lai M. Rab5 and class III phosphoinositide 3-kinase Vps34 are involved in hepatitis C virus NS4B-induced autophagy. J Virol. 2011;85:10561-71 pubmed publisher
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Sims J, Wade P. Mi-2/NuRD complex function is required for normal S phase progression and assembly of pericentric heterochromatin. Mol Biol Cell. 2011;22:3094-102 pubmed publisher
  • western blot; pigs ; fig 4
Yun Hong Y, Chih Fan C, Chia Wei C, Yen Chung C. A study of the spatial protein organization of the postsynaptic density isolated from porcine cerebral cortex and cerebellum. Mol Cell Proteomics. 2011;10:M110.007138 pubmed publisher
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Hsu F, Yang M, Lin E, Tseng C, Lin H. The significance of Her2 on androgen receptor protein stability in the transition of androgen requirement in prostate cancer cells. Am J Physiol Endocrinol Metab. 2011;300:E902-8 pubmed publisher
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Shi Chen Ou D, Lee S, Chu C, Chang L, Chung B, Juan L. Transcriptional activation of endoplasmic reticulum chaperone GRP78 by HCMV IE1-72 protein. Cell Res. 2011;21:642-53 pubmed publisher
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Su J, Gorse K, Ramirez F, Fox M. Collagen XIX is expressed by interneurons and contributes to the formation of hippocampal synapses. J Comp Neurol. 2010;518:229-53 pubmed publisher
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Jin L, Zhang G, Jamison C, Takano H, Haydon P, Selzer M. Axon regeneration in the absence of growth cones: acceleration by cyclic AMP. J Comp Neurol. 2009;515:295-312 pubmed publisher
Edwards G, Lee Y, Kim M, Bhanvadia S, Kim K, Ju W. Effect of Ubiquinol on Glaucomatous Neurodegeneration and Oxidative Stress: Studies for Retinal Ganglion Cell Survival and/or Visual Function. Antioxidants (Basel). 2020;9: pubmed publisher
Moriwaki Y, Kubo N, Watanabe M, Asano S, Shinoda T, Sugino T, et al. Endogenous neurotoxin-like protein Ly6H inhibits alpha7 nicotinic acetylcholine receptor currents at the plasma membrane. Sci Rep. 2020;10:11996 pubmed publisher
Varderidou Minasian S, Hinz L, Hagemans D, Posthuma D, Altelaar M, Heine V. Quantitative proteomic analysis of Rett iPSC-derived neuronal progenitors. Mol Autism. 2020;11:38 pubmed publisher
Lauriola A, Martello A, Fantini S, Marverti G, Zanocco Marani T, Davalli P, et al. Depletion of Trichoplein (TpMs) Causes Chromosome Mis-Segregation, DNA Damage and Chromosome Instability in Cancer Cells. Cancers (Basel). 2020;12: pubmed publisher
Fu X, Peng J, Wang A, Luo Z. Tumor necrosis factor alpha mediates neuromuscular synapse elimination. Cell Discov. 2020;6:9 pubmed publisher
Nguyen T, Ralbovska A, Kugler J. RhoBTB Proteins Regulate the Hippo Pathway by Antagonizing Ubiquitination of LKB1. G3 (Bethesda). 2020;10:1319-1325 pubmed publisher
Khandelwal S, Boylan M, Kirsch G, Spallholz J, Gollahon L. Investigating the Potential of Conjugated Selenium Redox Folic Acid as a Treatment for Triple Negative Breast Cancer. Antioxidants (Basel). 2020;9: pubmed publisher
Lohard S, Bourgeois N, Maillet L, Gautier F, Fétiveau A, Lasla H, et al. STING-dependent paracriny shapes apoptotic priming of breast tumors in response to anti-mitotic treatment. Nat Commun. 2020;11:259 pubmed publisher
Lee M, Liu Y, Chen C, Lu C, Lu S, Huang T, et al. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. J Cell Biol. 2020;219: pubmed publisher
Kurihara M, Mano T, Saito Y, Murayama S, Toda T, Iwata A. Colocalization of BRCA1 with Tau Aggregates in Human Tauopathies. Brain Sci. 2019;10: pubmed publisher
Fifield B, Qemo I, Kirou E, Cardiff R, Porter L. The atypical cyclin-like protein Spy1 overrides p53-mediated tumour suppression and promotes susceptibility to breast tumourigenesis. Breast Cancer Res. 2019;21:140 pubmed publisher
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Chen C, Ho Y, Ho H, Liang L, Jiang W, Lee G, et al. Tryptophan metabolite 5-methoxytryptophan ameliorates arterial denudation-induced intimal hyperplasia via opposing effects on vascular endothelial and smooth muscle cells. Aging (Albany NY). 2019;11:8604-8622 pubmed publisher
Costa R, Peruzzo R, Bachmann M, Montà G, Vicario M, Santinon G, et al. Impaired Mitochondrial ATP Production Downregulates Wnt Signaling via ER Stress Induction. Cell Rep. 2019;28:1949-1960.e6 pubmed publisher
Mariani M, Dasmeh P, Fortin A, Caron E, Kalamujic M, Harrison A, et al. The Combination of IFN β and TNF Induces an Antiviral and Immunoregulatory Program via Non-Canonical Pathways Involving STAT2 and IRF9. Cells. 2019;8: pubmed publisher
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Joyce M, Berry Wynne K, Dos Santos T, Addison W, McFarlane N, Hobman T, et al. HCV and flaviviruses hijack cellular mechanisms for nuclear STAT2 degradation: Up-regulation of PDLIM2 suppresses the innate immune response. PLoS Pathog. 2019;15:e1007949 pubmed publisher
Rushing A, Rushing B, Hoang K, Sanders S, Peloponese J, Polakowski N, et al. HTLV-1 basic leucine zipper factor protects cells from oxidative stress by upregulating expression of Heme Oxygenase I. PLoS Pathog. 2019;15:e1007922 pubmed publisher
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Kenny T, Craig A, Villanueva A, Germain D. Mitohormesis Primes Tumor Invasion and Metastasis. Cell Rep. 2019;27:2292-2303.e6 pubmed publisher
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Cartier E, Garcia Olivares J, Janezic E, Viana J, Moore M, Lin M, et al. The SUMO-Conjugase Ubc9 Prevents the Degradation of the Dopamine Transporter, Enhancing Its Cell Surface Level and Dopamine Uptake. Front Cell Neurosci. 2019;13:35 pubmed publisher
Gentili M, Lahaye X, Nadalin F, Nader G, Puig Lombardi E, Hervé S, et al. The N-Terminal Domain of cGAS Determines Preferential Association with Centromeric DNA and Innate Immune Activation in the Nucleus. Cell Rep. 2019;26:2377-2393.e13 pubmed publisher
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Chen L, Lin Y, Gallegos D, Hazlett M, Gómez Schiavon M, Yang M, et al. Enhancer Histone Acetylation Modulates Transcriptional Bursting Dynamics of Neuronal Activity-Inducible Genes. Cell Rep. 2019;26:1174-1188.e5 pubmed publisher
Taylor B, Sinha G, Donahue R, Grachen C, Morón J, Doolen S. Opioid receptors inhibit the spinal AMPA receptor Ca2+ permeability that mediates latent pain sensitization. Exp Neurol. 2019;314:58-66 pubmed publisher
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product information
Catalog Number :
MAB1501
Subcategory :
Cell Structure
Product Name :
Anti-Actin Antibody, clone C4
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P68133
Host Name :
Mouse
Antigen :
Actin
Clone :
C4
Conjugate :
Ascites
Isotype :
IgG1κ
Product Description :
Anti-Actin Antibody, clone C4
Cross Reactivity :
All
Background :
Actin is an abundant cytoskeletal protein found in all cells (True, 1990). The protein's 42 kD peptide chain assumes two physical forms: globular actin, which may serve as a cytoplasmic storage pool, and fibrous actin, which, in conjunction with myosin, generates muscle contraction (True, 1990). In non-muscle cells, actin appears to be involved in a variety of functions, such as cell motility, exocytosis, and phagocytosis (True, 1990). Distribution of the six known isoforms of actin - four muscle actins (alpha-skeletal, alpha-vascular smooth, alpha-cardiac, and gamma-enteric smooth) and two cytoplasmic actins (alpha and gamma) is tissue specific (Otey, 1986; Lessard, 1988).
ALT Names :
MAB1501X;MAB1501R
Immunogen :
Purified chicken gizzard actin (Lessard, 1988).
Specificity :
MAB1501 is a pan-actin antibody that binds to an epitope in a highly conserved region of actin; therefore, this antibody reacts with all six isoforms of vertabrate actin (Lessard, 1988). The epitope recognized by the antibody appears to be located in the N-terminal two thirds of the actin molecule, possibly near amino acids 50-70. Reacts with both globular (G) and fillimentous (F) forms of actin and does not interfere with actin polymerization to form filaments, at a ratio as high as one antibody per two actin monomers. However, this antibody does increase the extent of polymerization when used at a lower ratio of antibody to actin. In addition to labeling myotubes, anti-actin stains myoblasts and fibroblasts (Lessard, 1983). Although clone C4 is prepared as an antibody to chicken gizzard muscles actin, it reacts with actins from all vertebrates, as well as with Dictyostelium discoideum and Physarum polycephalum actins (Lessard, 1988).
Package Size :
100 µL
Uses :
ELISA;Immunocytochemistry;Immunofluorescence;Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting
Storage :
Stable at -20°C in undiluted aliquots for up to 12 months from date of receipt. Do not store in a diluted format. Avoid repeated freeze/thaw cycles.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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