This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
PECAM-1 Antibody, clone 2H8, Azide Free
catalog :
MAB1398Z
quantity :
100 μg
clonality :
monoclonal
host :
hamsters
conjugate :
nonconjugated
clone name :
2H8

The same clone is also sold as:
  • LifeSpan Biosciences: LS-C43526
  • Invitrogen: MA3105
  • Developmental Studies Hybridoma Bank: 2H8
reactivity :
human, mouse
application :
immunohistochemistry, neutralization, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry knockout validation
citations: 49
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry knockout validation; mouse; fig 4
Baluk P, Fuxe J, Hashizume H, Romano T, Lashnits E, Butz S, et al. Functionally specialized junctions between endothelial cells of lymphatic vessels. J Exp Med. 2007;204:2349-62 pubmed
  • immunohistochemistry - paraffin section; mouse; loading ...; fig 5a
  • immunohistochemistry; mouse; loading ...; fig 5b
Chakarov S, Lim H, Tan L, Lim S, See P, Lum J, et al. Two distinct interstitial macrophage populations coexist across tissues in specific subtissular niches. Science. 2019;363: pubmed publisher
  • immunohistochemistry - frozen section; human; loading ...; fig 1a
  • immunohistochemistry - frozen section; mouse; loading ...; fig s1a
Kim J, Park J, Choi J, Park I, Hwang Y, Bae H, et al. Tie2 activation promotes choriocapillary regeneration for alleviating neovascular age-related macular degeneration. Sci Adv. 2019;5:eaau6732 pubmed publisher
  • immunohistochemistry; mouse; 1:200; loading ...; fig 1h
Louveau A, Herz J, Alme M, Salvador A, Dong M, Viar K, et al. CNS lymphatic drainage and neuroinflammation are regulated by meningeal lymphatic vasculature. Nat Neurosci. 2018;21:1380-1391 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 1a
Dá Mesquita S, Louveau A, Vaccari A, Smirnov I, Cornelison R, Kingsmore K, et al. Functional aspects of meningeal lymphatics in ageing and Alzheimer's disease. Nature. 2018;560:185-191 pubmed publisher
  • immunohistochemistry - frozen section; human; loading ...; fig 2a
Kim J, Park D, Bae H, Park D, Kim D, Lee C, et al. Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma. J Clin Invest. 2017;127:3877-3896 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 1d
Kim J, Kim Y, Kim J, Park D, Bae H, Lee D, et al. YAP/TAZ regulates sprouting angiogenesis and vascular barrier maturation. J Clin Invest. 2017;127:3441-3461 pubmed publisher
  • immunohistochemistry; mouse; 1:200; fig 3
Incio J, Liu H, Suboj P, Chin S, Chen I, Pinter M, et al. Obesity-Induced Inflammation and Desmoplasia Promote Pancreatic Cancer Progression and Resistance to Chemotherapy. Cancer Discov. 2016;6:852-69 pubmed publisher
  • immunohistochemistry; mouse; 1:200; fig 5
Kim Y, Nam H, Lee J, Park D, Kim C, Yu Y, et al. Methylation-dependent regulation of HIF-1α stability restricts retinal and tumour angiogenesis. Nat Commun. 2016;7:10347 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; fig 7
Cardoso F, Herz J, Fernandes A, Rocha J, Sepodes B, Brito M, et al. Systemic inflammation in early neonatal mice induces transient and lasting neurodegenerative effects. J Neuroinflammation. 2015;12:82 pubmed publisher
  • immunohistochemistry; mouse; 1:2000; fig 6
Hakanpaa L, Sipilä T, Leppänen V, Gautam P, Nurmi H, Jacquemet G, et al. Endothelial destabilization by angiopoietin-2 via integrin β1 activation. Nat Commun. 2015;6:5962 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100
Chucair Elliott A, Zheng M, Carr D. Degeneration and regeneration of corneal nerves in response to HSV-1 infection. Invest Ophthalmol Vis Sci. 2015;56:1097-107 pubmed publisher
  • immunohistochemistry; mouse
Fink D, Connor A, Kelley P, Steele M, Hollingsworth M, Tempero R. Nerve growth factor regulates neurolymphatic remodeling during corneal inflammation and resolution. PLoS ONE. 2014;9:e112737 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:400
Hayakawa K, Okazaki R, Morioka K, Nakamura K, Tanaka S, Ogata T. Lipopolysaccharide preconditioning facilitates M2 activation of resident microglia after spinal cord injury. J Neurosci Res. 2014;92:1647-58 pubmed publisher
  • immunohistochemistry; mouse; 1:1000
Lu Z, Kaliberov S, Zhang J, Muz B, Azab A, Sohn R, et al. The myeloid-binding peptide adenoviral vector enables multi-organ vascular endothelial gene targeting. Lab Invest. 2014;94:881-92 pubmed publisher
  • immunohistochemistry; human
Donat U, Rother J, Schäfer S, Hess M, Härtl B, Kober C, et al. Characterization of metastasis formation and virotherapy in the human C33A cervical cancer model. PLoS ONE. 2014;9:e98533 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:50
Song K, Chung J, Choi M, Jin H, Yin G, Kwon M, et al. Effectiveness of intracavernous delivery of adenovirus encoding Smad7 gene on erectile function in a mouse model of cavernous nerve injury. J Sex Med. 2014;11:51-63 pubmed publisher
  • immunohistochemistry - frozen section; mouse
Maddaluno L, Rudini N, Cuttano R, Bravi L, Giampietro C, Corada M, et al. EndMT contributes to the onset and progression of cerebral cavernous malformations. Nature. 2013;498:492-6 pubmed publisher
  • immunohistochemistry - frozen section; human
Donat U, Weibel S, Hess M, Stritzker J, Härtl B, Sturm J, et al. Preferential colonization of metastases by oncolytic vaccinia virus strain GLV-1h68 in a human PC-3 prostate cancer model in nude mice. PLoS ONE. 2012;7:e45942 pubmed publisher
Zheng W, Zhang H, Zhao D, Zhang J, Pollard J. Lung Mammary Metastases but Not Primary Tumors Induce Accumulation of Atypical Large Platelets and Their Chemokine Expression. Cell Rep. 2019;29:1747-1755.e4 pubmed publisher
Hong S, Song S, Lee S, Jo H, Kim H, Han J, et al. Regenerative potential of mouse embryonic stem cell-derived PDGFRα+ cardiac lineage committed cells in infarcted myocardium. World J Stem Cells. 2019;11:44-54 pubmed publisher
Villahoz S, Yunes Leites P, Méndez Barbero N, Urso K, Bonzon Kulichenko E, Ortega S, et al. Conditional deletion of Rcan1 predisposes to hypertension-mediated intramural hematoma and subsequent aneurysm and aortic rupture. Nat Commun. 2018;9:4795 pubmed publisher
Nawaz A, Mehmood A, Kanatani Y, Kado T, Igarashi Y, Takikawa A, et al. Sirt1 activator induces proangiogenic genes in preadipocytes to rescue insulin resistance in diet-induced obese mice. Sci Rep. 2018;8:11370 pubmed publisher
Kuo A, Lee M, Yang K, Gross R, Sessa W. Caveolin-1 regulates lipid droplet metabolism in endothelial cells via autocrine prostacyclin-stimulated, cAMP-mediated lipolysis. J Biol Chem. 2018;293:973-983 pubmed publisher
Chucair Elliott A, Gurung H, Carr M, Carr D. Colony Stimulating Factor-1 Receptor Expressing Cells Infiltrating the Cornea Control Corneal Nerve Degeneration in Response to HSV-1 Infection. Invest Ophthalmol Vis Sci. 2017;58:4670-4682 pubmed publisher
Chucair Elliott A, Carr M, Carr D. Long-term consequences of topical dexamethasone treatment during acute corneal HSV-1 infection on the immune system. J Leukoc Biol. 2017;101:1253-1261 pubmed publisher
Santeford A, Wiley L, Park S, Bamba S, Nakamura R, Gdoura A, et al. Impaired autophagy in macrophages promotes inflammatory eye disease. Autophagy. 2016;12:1876-1885 pubmed
Yuan L, Chan G, Beeler D, Janes L, Spokes K, Dharaneeswaran H, et al. A role of stochastic phenotype switching in generating mosaic endothelial cell heterogeneity. Nat Commun. 2016;7:10160 pubmed publisher
Connor A, Kelley P, Tempero R. Lymphatic endothelial lineage assemblage during corneal lymphangiogenesis. Lab Invest. 2016;96:270-82 pubmed publisher
Cuttano R, Rudini N, Bravi L, Corada M, Giampietro C, Papa E, et al. KLF4 is a key determinant in the development and progression of cerebral cavernous malformations. EMBO Mol Med. 2016;8:6-24 pubmed publisher
Askoxylakis V, Ferraro G, Kodack D, Badeaux M, Shankaraiah R, Seano G, et al. Preclinical Efficacy of Ado-trastuzumab Emtansine in the Brain Microenvironment. J Natl Cancer Inst. 2016;108: pubmed publisher
Cruz F, Tomé M, Bernal J, Bernad A. miR-300 mediates Bmi1 function and regulates differentiation in primitive cardiac progenitors. Cell Death Dis. 2015;6:e1953 pubmed publisher
Wong H, Fatimy R, Onodera C, Wei Z, Yi M, Mohan A, et al. The Cancer Genome Atlas Analysis Predicts MicroRNA for Targeting Cancer Growth and Vascularization in Glioblastoma. Mol Ther. 2015;23:1234-1247 pubmed publisher
Sørensen B, Busk M, Horsman M, Alsner J, Overgaard J, Kyle A, et al. Effect of radiation on cell proliferation and tumor hypoxia in HPV-positive head and neck cancer in vivo models. Anticancer Res. 2014;34:6297-304 pubmed
Park S, Cha J, Nam J, Kim M, Park S, Park E, et al. Formulation optimization and in vivo proof-of-concept study of thermosensitive liposomes balanced by phospholipid, elastin-like polypeptide, and cholesterol. PLoS ONE. 2014;9:e103116 pubmed publisher
Guérit S, Allain A, Léon C, Cazenave W, Ferrara N, Branchereau P, et al. VEGF modulates synaptic activity in the developing spinal cord. Dev Neurobiol. 2014;74:1110-22 pubmed publisher
Michael I, Westenskow P, Hacibekiroglu S, Greenwald A, Ballios B, Kurihara T, et al. Local acting Sticky-trap inhibits vascular endothelial growth factor dependent pathological angiogenesis in the eye. EMBO Mol Med. 2014;6:604-23 pubmed publisher
Lu Z, Kaliberov S, Sohn R, Kaliberova L, Curiel D, Arbeit J. Transcriptional targeting of primary and metastatic tumor neovasculature by an adenoviral type 5 roundabout4 vector in mice. PLoS ONE. 2013;8:e83933 pubmed publisher
Ryu J, Kim D, Song K, Yi T, Suh J, Song S. Intracavernous delivery of clonal mesenchymal stem cells restores erectile function in a mouse model of cavernous nerve injury. J Sex Med. 2014;11:411-23 pubmed publisher
Shi Y, Tu Y, Mecham R, Bassnett S. Ocular phenotype of Fbn2-null mice. Invest Ophthalmol Vis Sci. 2013;54:7163-73 pubmed publisher
Ryu M, Kim S, Choi M, Kim Y, Lee M, Lee S, et al. Mitochondrial oxidative phosphorylation reserve is required for hormone- and PPAR? agonist-induced adipogenesis. Mol Cells. 2013;35:134-41 pubmed publisher
Anderberg C, Cunha S, Zhai Z, Cortez E, Pardali E, Johnson J, et al. Deficiency for endoglin in tumor vasculature weakens the endothelial barrier to metastatic dissemination. J Exp Med. 2013;210:563-79 pubmed publisher
Patil S, Gentschev I, Adelfinger M, Donat U, Hess M, Weibel S, et al. Virotherapy of canine tumors with oncolytic vaccinia virus GLV-1h109 expressing an anti-VEGF single-chain antibody. PLoS ONE. 2012;7:e47472 pubmed publisher
Schäfer S, Weibel S, Donat U, Zhang Q, Aguilar R, Chen N, et al. Vaccinia virus-mediated intra-tumoral expression of matrix metalloproteinase 9 enhances oncolysis of PC-3 xenograft tumors. BMC Cancer. 2012;12:366 pubmed publisher
Gentschev I, Adelfinger M, Josupeit R, Rudolph S, Ehrig K, Donat U, et al. Preclinical evaluation of oncolytic vaccinia virus for therapy of canine soft tissue sarcoma. PLoS ONE. 2012;7:e37239 pubmed publisher
Mukouyama Y, James J, Nam J, Uchida Y. Whole-mount confocal microscopy for vascular branching morphogenesis. Methods Mol Biol. 2012;843:69-78 pubmed publisher
Kelley P, Steele M, Tempero R. Regressed lymphatic vessels develop during corneal repair. Lab Invest. 2011;91:1643-51 pubmed publisher
Zhang L, Zhou F, Han W, Shen B, Luo J, Shibuya M, et al. VEGFR-3 ligand-binding and kinase activity are required for lymphangiogenesis but not for angiogenesis. Cell Res. 2010;20:1319-31 pubmed publisher
Tanabe K, Maeshima Y, Ichinose K, Kitayama H, Takazawa Y, Hirokoshi K, et al. Endostatin peptide, an inhibitor of angiogenesis, prevents the progression of peritoneal sclerosis in a mouse experimental model. Kidney Int. 2007;71:227-38 pubmed
product information
Catalog Number :
MAB1398Z
Subcategory :
Cell Structure
Product Name :
Anti-PECAM-1 Antibody, clone 2H8, Azide Free
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P69905
Host Name :
Armenian Hamster
Antigen :
PECAM-1
Clone :
2H8
Conjugate :
Purified
Isotype :
IgG
Product Description :
Anti-PECAM-1 Antibody, clone 2H8, Azide Free
Cross Reactivity :
Mouse
Background :
CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
ALT Names :
CD31;MAB1398
Specificity :
React s with the 130-140 kDa protein, PECAM-1 found on mouse monocytes, platelets, granulocytes and endothelial cells. 2H8 effectively inhibits transmigration of activated polymophonuclear cells and monocytes across the endothelium. In a mouse model for acute peritonis - 2H8 blocks acute inflammation.
Package Size :
100 μg
Uses :
Flow Cytometry;Affects Function
Storage :
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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