Integrin α6 Antibody, clone NKI-GoH3 | EMD Millipore MAB1378 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Integrin α6 Antibody, clone NKI-GoH3

catalog :

MAB1378

quantity :

100 μg

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

NKI-GoH3

The same clone is also sold as:

LifeSpan Biosciences: LS-C45281, LS-C45279, LS-C45280
MyBioSource: MBS210508, MBS211073
OriGene: SM038R, SM038PS, SM038P, BM5115, SM038F, SM038PT
Santa Cruz Biotechnology: sc-80554
Invitrogen: MA5-16884, MA5-16885
Bio-Rad: MCA699GA, MCA699T, MCA699A647, MCA699PE, MCA699, MCA699F

reactivity :

human, mouse

application :

immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, blocking or activating experiments

citations: 50

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:100; fig 1a	Reynolds L, D Amico G, Lechertier T, Papachristodoulou A, Muñoz Félix J, De Arcangelis A, et al. Dual role of pericyte ?6?1-integrin in tumour blood vessels. J Cell Sci. 2017;130:1583-1595 pubmed publisher
immunocytochemistry; human	Hild M, Jaffe A. Production of 3-D Airway Organoids From Primary Human Airway Basal Cells and Their Use in High-Throughput Screening. Curr Protoc Stem Cell Biol. 2016;37:IE.9.1-IE.9.15 pubmed publisher
immunocytochemistry; human; fig 4	Schnerch D, Nigg E. Structural centrosome aberrations favor proliferation by abrogating microtubule-dependent tissue integrity of breast epithelial mammospheres. Oncogene. 2016;35:2711-22 pubmed publisher
immunohistochemistry - paraffin section; mouse; loading ...; fig 6a	Basak P, Dillon R, Leslie H, Raouf A, Mowat M. The Deleted in Liver Cancer 1 (Dlc1) tumor suppressor is haploinsufficient for mammary gland development and epithelial cell polarity. BMC Cancer. 2015;15:630 pubmed publisher
blocking or activating experiments; human	Toh Y, Xing J, Yu H. Modulation of integrin and E-cadherin-mediated adhesions to spatially control heterogeneity in human pluripotent stem cell differentiation. Biomaterials. 2015;50:87-97 pubmed publisher
immunocytochemistry; human; fig 1	Kocher B, White L, Piwnica Worms D. DAPK3 suppresses acini morphogenesis and is required for mouse development. Mol Cancer Res. 2015;13:358-67 pubmed publisher
flow cytometry; human; 1:200	Persson B, Jaffe A, Fearns R, Danahay H. Respiratory syncytial virus can infect basal cells and alter human airway epithelial differentiation. PLoS ONE. 2014;9:e102368 pubmed publisher
flow cytometry; mouse; 1:10	Petrakova O, Terskikh V, Chernioglo E, Ashapkin V, Bragin E, Shtratnikova V, et al. Comparative analysis reveals similarities between cultured submandibular salivary gland cells and liver progenitor cells. Springerplus. 2014;3:183 pubmed publisher
blocking or activating experiments; human; 10 ug/ml flow cytometry; human; 2 ug/25 x104cells	Stampolidis P, Ullrich A, Iacobelli S. LGALS3BP, lectin galactoside-binding soluble 3 binding protein, promotes oncogenic cellular events impeded by antibody intervention. Oncogene. 2015;34:39-52 pubmed publisher
flow cytometry; mouse	Shin E, Sorenson C, Sheibani N. PEDF expression regulates the proangiogenic and proinflammatory phenotype of the lung endothelium. Am J Physiol Lung Cell Mol Physiol. 2014;306:L620-34 pubmed publisher
flow cytometry; mouse; 1:200	Zhao Y, Wang S, Sorenson C, Teixeira L, Dubielzig R, Peters D, et al. Cyp1b1 mediates periostin regulation of trabecular meshwork development by suppression of oxidative stress. Mol Cell Biol. 2013;33:4225-40 pubmed publisher
immunohistochemistry - frozen section; human; 1:100 flow cytometry; human	Bridgen D, Gilchrist C, Richardson W, Isaacs R, Brown C, Yang K, et al. Integrin-mediated interactions with extracellular matrix proteins for nucleus pulposus cells of the human intervertebral disc. J Orthop Res. 2013;31:1661-7 pubmed publisher
	Alladin A, Chaible L, Garcia Del Valle L, Sabine R, Loeschinger M, Wachsmuth M, et al. Tracking cells in epithelial acini by light sheet microscopy reveals proximity effects in breast cancer initiation. elife. 2020;9: pubmed publisher
	Bazzoun D, Adissu H, Wang L, Urazaev A, Tenvooren I, Fostok S, et al. Connexin 43 maintains tissue polarity and regulates mitotic spindle orientation in the breast epithelium. J Cell Sci. 2019;132: pubmed publisher
	Knouse K, Lopez K, Bachofner M, Amon A. Chromosome Segregation Fidelity in Epithelia Requires Tissue Architecture. Cell. 2018;175:200-211.e13 pubmed publisher
	Campbell P, Mavingire N, Khan S, Rowland L, Wooten J, Opoku Agyeman A, et al. AhR ligand aminoflavone suppresses α6-integrin-Src-Akt signaling to attenuate tamoxifen resistance in breast cancer cells. J Cell Physiol. 2018;234:108-121 pubmed publisher
	Wang Z, Plasschaert L, Aryal S, Renaud N, Yang Z, Choo Wing R, et al. TRRAP is a central regulator of human multiciliated cell formation. J Cell Biol. 2018;217:1941-1955 pubmed publisher
	Lee T, Kim Y, Zhang W, Song I, Tung C. Facile metabolic glycan labeling strategy for exosome tracking. Biochim Biophys Acta Gen Subj. 2018;1862:1091-1100 pubmed publisher
	Valcourt U, Carthy J, Okita Y, Alcaraz L, Kato M, Thuault S, et al. Analysis of Epithelial-Mesenchymal Transition Induced by Transforming Growth Factor Î². Methods Mol Biol. 2016;1344:147-81 pubmed publisher
	Todhunter M, Jee N, Hughes A, Coyle M, Cerchiari A, Farlow J, et al. Programmed synthesis of three-dimensional tissues. Nat Methods. 2015;12:975-81 pubmed publisher
	Pageon H, Zucchi H, Dai Z, Sell D, Strauch C, Monnier V, et al. Biological Effects Induced by Specific Advanced Glycation End Products in the Reconstructed Skin Model of Aging. Biores Open Access. 2015;4:54-64 pubmed publisher
	Wei S, Fattet L, Tsai J, Guo Y, Pai V, Majeski H, et al. Matrix stiffness drives epithelial-mesenchymal transition and tumour metastasis through a TWIST1-G3BP2 mechanotransduction pathway. Nat Cell Biol. 2015;17:678-88 pubmed publisher
	Melo S, Lisowski L, Bashkirova E, Zhen H, Chu K, Keene D, et al. Somatic correction of junctional epidermolysis bullosa by a highly recombinogenic AAV variant. Mol Ther. 2014;22:725-33 pubmed publisher
	Diaferia G, Cirulli V, Biunno I. SEL1L regulates adhesion, proliferation and secretion of insulin by affecting integrin signaling. PLoS ONE. 2013;8:e79458 pubmed publisher
	Bray K, Gillette M, Young J, Loughran E, Hwang M, Sears J, et al. Cdc42 overexpression induces hyperbranching in the developing mammary gland by enhancing cell migration. Breast Cancer Res. 2013;15:R91 pubmed
	Kwon J, Lee T, Lee H, Kang M, Yoon H, Kim J, et al. Integrin alpha 6: a novel therapeutic target in esophageal squamous cell carcinoma. Int J Oncol. 2013;43:1523-30 pubmed publisher
	Kothapalli C, Kamm R. 3D matrix microenvironment for targeted differentiation of embryonic stem cells into neural and glial lineages. Biomaterials. 2013;34:5995-6007 pubmed publisher
	Schumann H, Kiritsi D, Pigors M, Hausser I, Kohlhase J, Peters J, et al. Phenotypic spectrum of epidermolysis bullosa associated with ?6?4 integrin mutations. Br J Dermatol. 2013;169:115-24 pubmed publisher
	Sukhdeo K, Paramban R, Vidal J, Elia J, Martin J, Rivera M, et al. Multiplex flow cytometry barcoding and antibody arrays identify surface antigen profiles of primary and metastatic colon cancer cell lines. PLoS ONE. 2013;8:e53015 pubmed publisher
	Lee E, Woo J, Hwang J, Park J, Cho C. Angiopoietin 1 enhances the proliferation and differentiation of skeletal myoblasts. J Cell Physiol. 2013;228:1038-44 pubmed publisher
	Fennewald S, Kantara C, Sastry S, Resto V. Laminin interactions with head and neck cancer cells under low fluid shear conditions lead to integrin activation and binding. J Biol Chem. 2012;287:21058-66 pubmed publisher
	Zhang H, Guo F, Zhu G. Involvement of host cell integrin ?2 in Cryptosporidium parvum infection. Infect Immun. 2012;80:1753-8 pubmed publisher
	Bray K, Brakebusch C, Vargo Gogola T. The Rho GTPase Cdc42 is required for primary mammary epithelial cell morphogenesis in vitro. Small Gtpases. 2011;2:247-258 pubmed
	Qiu L, Low H, Chang C, Strohsnitter W, Anderson M, Edmiston K, et al. Novel measurements of mammary stem cells in human umbilical cord blood as prospective predictors of breast cancer susceptibility in later life. Ann Oncol. 2012;23:245-50 pubmed publisher
	Zhu H, Riau A, Beuerman R. Epithelial microfilament regulators show regional distribution in mouse conjunctiva. Mol Vis. 2010;16:2215-24 pubmed
	DeRouen M, Zhen H, Tan S, Williams S, Marinkovich M, Oro A. Laminin-511 and integrin beta-1 in hair follicle development and basal cell carcinoma formation. BMC Dev Biol. 2010;10:112 pubmed publisher
	Gonzalez Suarez E, Jacob A, Jones J, Miller R, Roudier Meyer M, Erwert R, et al. RANK ligand mediates progestin-induced mammary epithelial proliferation and carcinogenesis. Nature. 2010;468:103-7 pubmed publisher
	Eastman R, Leaf E, Zhang D, True L, Sweet R, Seidel K, et al. Fibroblast growth factor-10 signals development of von Brunn's nests in the exstrophic bladder. Am J Physiol Renal Physiol. 2010;299:F1094-110 pubmed publisher
	Taraseviciute A, Vincent B, Schedin P, Jones P. Quantitative analysis of three-dimensional human mammary epithelial tissue architecture reveals a role for tenascin-C in regulating c-met function. Am J Pathol. 2010;176:827-38 pubmed publisher
	Zhang X, Ladd A, Dragoescu E, Budd W, Ware J, Zehner Z. MicroRNA-17-3p is a prostate tumor suppressor in vitro and in vivo, and is decreased in high grade prostate tumors analyzed by laser capture microdissection. Clin Exp Metastasis. 2009;26:965-79 pubmed publisher
	Jechlinger M, Podsypanina K, Varmus H. Regulation of transgenes in three-dimensional cultures of primary mouse mammary cells demonstrates oncogene dependence and identifies cells that survive deinduction. Genes Dev. 2009;23:1677-88 pubmed publisher
	Barbolina M, Adley B, Kelly D, Shepard J, Fought A, Scholtens D, et al. Downregulation of connective tissue growth factor by three-dimensional matrix enhances ovarian carcinoma cell invasion. Int J Cancer. 2009;125:816-25 pubmed publisher
	Zhang X, Fournier M, Ware J, Bissell M, Yacoub A, Zehner Z. Inhibition of vimentin or beta1 integrin reverts morphology of prostate tumor cells grown in laminin-rich extracellular matrix gels and reduces tumor growth in vivo. Mol Cancer Ther. 2009;8:499-508 pubmed publisher
	Gendronneau G, Sidhu S, Delacour D, Dang T, Calonne C, Houzelstein D, et al. Galectin-7 in the control of epidermal homeostasis after injury. Mol Biol Cell. 2008;19:5541-9 pubmed publisher
	Lathia J, Patton B, Eckley D, Magnus T, Mughal M, Sasaki T, et al. Patterns of laminins and integrins in the embryonic ventricular zone of the CNS. J Comp Neurol. 2007;505:630-43 pubmed
	Sheibani N, Scheef E, Dimaio T, Wang Y, Kondo S, Sorenson C. Bcl-2 expression modulates cell adhesion and migration promoting branching of ureteric bud cells. J Cell Physiol. 2007;210:616-25 pubmed
	Maginnis M, Forrest J, Kopecky Bromberg S, Dickeson S, Santoro S, Zutter M, et al. Beta1 integrin mediates internalization of mammalian reovirus. J Virol. 2006;80:2760-70 pubmed
	Whitlon D, Ketels K, Coulson M, Williams T, Grover M, Edpao W, et al. Survival and morphology of auditory neurons in dissociated cultures of newborn mouse spiral ganglion. Neuroscience. 2006;138:653-62 pubmed
	Todorovic V, Chen C, Hay N, Lau L. The matrix protein CCN1 (CYR61) induces apoptosis in fibroblasts. J Cell Biol. 2005;171:559-68 pubmed
	Pfarrer C. Characterization of the bovine placenta by cytoskeleton, integrin receptors, and extracellular matrix. Methods Mol Med. 2006;121:323-35 pubmed

product information

Catalog Number :

MAB1378

Subcategory :

Cell Structure

Product Name :

Anti-Integrin α6 Antibody, clone NKI-GoH3

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P23229

Host Name :

Rat

Antigen :

Integrin α6

Clone :

NKI-GoH3

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-Integrin α6 Antibody, clone NKI-GoH3

Cross Reactivity :

Human;Mouse

Background :

Integrins are a family of dimeric, transmembrane proteins that mediate cell-cell and extracellular matrix adhesion. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. The integrin family is composed of at least 15 alpha and 8 beta subunits that may form over twenty different alpha-beta non-covalently bound dimeric combinations on the cell surface. The alpha subunits all have some homology to each other, as do the beta subunits. Both of the subunits contribute to the binding of the ligand. Integrin alpha subunits contain seven weak sequence repeats in the N-terminal region, which may be important in ligand binding, and have been predicted to fold cooperatively into a single beta-propeller domain with seven beta-sheets. In normal tissue, Integrin alpha-6/beta-1 is a receptor for laminin on human platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Various disease states involving epithelial cells have been shown to be associated with alterations in alpha 6 integrin-containing heterodimers.

ALT Names :

CD49f

Immunogen :

Mammary tumor cells from BALB/c mice.

Specificity :

Directed against the Cdw49f-antigen, 120kDa, (GP Ic or VLA-6 alpha-chain), which can form distinct complexes with either the CD29-antigen (GP 11a or VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells. The monoclonal antibody reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytotrophoblasts. Platelet VL-6 functions as a laminin receptor and platelet adhesion to laminin is inhibited by GoH3.

Package Size :

100 μg

Uses :

Flow Cytometry;Immunohistochemistry;Affects Function

Storage :

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.