MMP-2 Proform Antibody, NT, clone CA-4001 | EMD Millipore MAB13405 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

MMP-2 Proform Antibody, NT, clone CA-4001

catalog :

MAB13405

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

CA-4001

reactivity :

hamsters, human

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section, blocking or activating experiments

citations: 24

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; fig 3	Roy R, Zurakowski D, Wischhusen J, Frauenhoffer C, Hooshmand S, Kulke M, et al. Urinary TIMP-1 and MMP-2 levels detect the presence of pancreatic malignancies. Br J Cancer. 2014;111:1772-9 pubmed publisher
blocking or activating experiments; human; 5 ug/ml; loading ...; fig 4a western blot; human; 1:1000; loading ...; fig st3	Rahme G, Israel M. Id4 suppresses MMP2-mediated invasion of glioblastoma-derived cells by direct inactivation of Twist1 function. Oncogene. 2015;34:53-62 pubmed publisher
western blot; hamsters; 1:1000; fig 2	Schiffmacher A, Padmanabhan R, Jhingory S, Taneyhill L. Cadherin-6B is proteolytically processed during epithelial-to-mesenchymal transitions of the cranial neural crest. Mol Biol Cell. 2014;25:41-54 pubmed publisher
western blot; human	Chik F, Machnes Z, Szyf M. Synergistic anti-breast cancer effect of a combined treatment with the methyl donor S-adenosyl methionine and the DNA methylation inhibitor 5-aza-2'-deoxycytidine. Carcinogenesis. 2014;35:138-44 pubmed publisher
	Hassona Y, Cirillo N, Heesom K, Parkinson E, Prime S. Senescent cancer-associated fibroblasts secrete active MMP-2 that promotes keratinocyte dis-cohesion and invasion. Br J Cancer. 2014;111:1230-7 pubmed publisher
	Bodnar M, Szylberg L, Kazmierczak W, Marszalek A. Differentiated expression of membrane type metalloproteinases (MMP-14, MMP-15) and pro-MMP2 in laryngeal squamous cell carcinoma. A novel mechanism. J Oral Pathol Med. 2013;42:267-74 pubmed publisher
	Kryczka J, Stasiak M, Dziki L, Mik M, Dziki A, Cierniewski C. Matrix metalloproteinase-2 cleavage of the ?1 integrin ectodomain facilitates colon cancer cell motility. J Biol Chem. 2012;287:36556-66 pubmed publisher
	Chen H, Yuan Y, Zhang C, Luo A, Ding F, Ma J, et al. Involvement of S100A14 protein in cell invasion by affecting expression and function of matrix metalloproteinase (MMP)-2 via p53-dependent transcriptional regulation. J Biol Chem. 2012;287:17109-19 pubmed publisher
	Gu W, Liu W, Yang X, Yuan X, Tian Y, Meng R, et al. Cutis laxa: analysis of metalloproteinases and extracellular matrix expression by immunohistochemistry and histochemistry. Eur J Dermatol. 2011;21:717-21 pubmed publisher
	Moore B, Manthey C, Johnson D, Bauer A. Matrix metalloproteinase-9 inhibition reduces inflammation and improves motility in murine models of postoperative ileus. Gastroenterology. 2011;141:1283-92, 1292.e1-4 pubmed publisher
	Shen Q, Lee E, Pitts R, Wu M, Yuan S. Tissue inhibitor of metalloproteinase-2 regulates matrix metalloproteinase-2-mediated endothelial barrier dysfunction and breast cancer cell transmigration through lung microvascular endothelial cells. Mol Cancer Res. 2010;8:939-51 pubmed publisher
	Glaeser J, Geissler S, Ode A, Schipp C, Matziolis G, Taylor W, et al. Modulation of matrix metalloprotease-2 levels by mechanical loading of three-dimensional mesenchymal stem cell constructs: impact on in vitro tube formation. Tissue Eng Part A. 2010;16:3139-48 pubmed publisher
	Sareen D, McMillan E, Ebert A, Shelley B, Johnson J, Meisner L, et al. Chromosome 7 and 19 trisomy in cultured human neural progenitor cells. PLoS ONE. 2009;4:e7630 pubmed publisher
	Mukhopadhyay U, Eves R, Jia L, Mooney P, Mak A. p53 suppresses Src-induced podosome and rosette formation and cellular invasiveness through the upregulation of caldesmon. Mol Cell Biol. 2009;29:3088-98 pubmed publisher
	Kappert K, Meyborg H, Baumann B, Furundzija V, Kaufmann J, Graf K, et al. Integrin cleavage facilitates cell surface-associated proteolysis required for vascular smooth muscle cell invasion. Int J Biochem Cell Biol. 2009;41:1511-7 pubmed publisher
	Alcazar O, Cousins S, Marin Castano M. MMP-14 and TIMP-2 overexpression protects against hydroquinone-induced oxidant injury in RPE: implications for extracellular matrix turnover. Invest Ophthalmol Vis Sci. 2007;48:5662-70 pubmed
	Jeyabalan A, Novak J, Doty K, Matthews J, Fisher M, Kerchner L, et al. Vascular matrix metalloproteinase-9 mediates the inhibition of myogenic reactivity in small arteries isolated from rats after short-term administration of relaxin. Endocrinology. 2007;148:189-97 pubmed
	Roesch A, Wittschier S, Becker B, Landthaler M, Vogt T. Loss of dipeptidyl peptidase IV immunostaining discriminates malignant melanomas from deep penetrating nevi. Mod Pathol. 2006;19:1378-85 pubmed
	Rolland Y, Demeule M, Beliveau R. Melanotransferrin stimulates t-PA-dependent activation of plasminogen in endothelial cells leading to cell detachment. Biochim Biophys Acta. 2006;1763:393-401 pubmed
	Kim M, Kim S. Chitooligosaccharides inhibit activation and expression of matrix metalloproteinase-2 in human dermal fibroblasts. FEBS Lett. 2006;580:2661-6 pubmed
	Muñoz Nájar U, Neurath K, Vumbaca F, Claffey K. Hypoxia stimulates breast carcinoma cell invasion through MT1-MMP and MMP-2 activation. Oncogene. 2006;25:2379-92 pubmed
	Yucel T, Mutnal A, Fay K, Fligiel S, Wang T, Johnson T, et al. Matrix metalloproteinase expression in basal cell carcinoma: relationship between enzyme profile and collagen fragmentation pattern. Exp Mol Pathol. 2005;79:151-60 pubmed
	Lalu M, Pasini E, Schulze C, Ferrari Vivaldi M, Ferrari Vivaldi G, Bachetti T, et al. Ischaemia-reperfusion injury activates matrix metalloproteinases in the human heart. Eur Heart J. 2005;26:27-35 pubmed
	Leroy Dudal J, Demeilliers C, Gallet O, Pauthe E, Dutoit S, Agniel R, et al. Transmigration of human ovarian adenocarcinoma cells through endothelial extracellular matrix involves alphav integrins and the participation of MMP2. Int J Cancer. 2005;114:531-43 pubmed

product information

Catalog Number :

MAB13405

Subcategory :

Cell Structure

Product Name :

Anti-MMP-2 Proform Antibody, NT, clone CA-4001

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P08253

Host Name :

Mouse

Antigen :

MMP-2 Proform

Clone :

CA-4001

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-MMP-2 Proform Antibody, NT, clone CA-4001

Cross Reactivity :

Human;Mouse;Rat

ALT Names :

Gelatinase A; 72 kDa Type IV Collagenase

Immunogen :

N-terminal peptide APSPIIKFPGDVAPKTDK of procollagenase IV, coupled to KLH.{Marqulies, et al 1992}

Specificity :

It recognizes a protein of 72 kDa which is identified as pro (latent) form (active form is 62 kDa) of matrix metalloproteinase-2 and any fragment with N-terminal (proform) domain still attached. Antibody shows no cross-reaction with pro and active forms of MMP-9. MMP-2 is synthesized as 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids. MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). Cellular Localization: Cytoplasmic.

Package Size :

100 µg

Uses :

Western Blotting;Immunohistochemistry (Paraffin);Affects Function

Storage :

Maintain at 2-8°C in undiluted aliquots for up to 12 months after date of receipt.