HLA-B27 Antibody, clone HLA.ABC.m3 | EMD Millipore MAB1285 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

HLA-B27 Antibody, clone HLA.ABC.m3

catalog :

MAB1285

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HLA.ABC.m3

The same clone is also sold as:

Bio-Rad: MCA116F
MyBioSource: MBS210626
Invitrogen: MA1-82540
Abcam: ab23840

reactivity :

human

application :

immunocytochemistry, flow cytometry

citations: 1

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 7b	Dellgren C, Ekwelum V, Ormhøj M, Pallesen N, Knudsen J, Nehlin J, et al. Low Constitutive Cell Surface Expression of HLA-B Is Caused by a Posttranslational Mechanism Involving Glu180 and Arg239. J Immunol. 2016;197:4807-4816 pubmed

product information

Catalog Number :

MAB1285

Subcategory :

Inflammation & Immunology

Product Name :

Anti-HLA-B27 Antibody, clone HLA.ABC.m3

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P03989

Host Name :

Mouse

Antigen :

HLA-B27

Clone :

HLA.ABC.m3

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-HLA-B27 Antibody, clone HLA.ABC.m3

Cross Reactivity :

Human

Immunogen :

Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA antibody and staphylococcal Protein A.

Specificity :

This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7 (9.5x107M-1). Cell reactivity: Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results: Subjects Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B7+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative

Package Size :

100 µg

Uses :

Flow Cytometry;Immunocytochemistry

Storage :

Store at 2 to 8°C. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES. WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.