DNA Antibody, double stranded, clone BV16-13 | EMD Millipore MAB030 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

DNA Antibody, double stranded, clone BV16-13

catalog :

MAB030

quantity :

500 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BV16-13

application :

immunocytochemistry, radioimmunoassay

citations: 5

Reference
Sasaki T, Sato Y, Higashiyama T, Sasaki N. Live imaging reveals the dynamics and regulation of mitochondrial nucleoids during the cell cycle in Fucci2-HeLa cells. Sci Rep. 2017;7:11257 pubmed publisher
Lamphier M, Zheng W, Latz E, Spyvee M, Hansen H, Rose J, et al. Novel small molecule inhibitors of TLR7 and TLR9: mechanism of action and efficacy in vivo. Mol Pharmacol. 2014;85:429-40 pubmed publisher
Papp K, Végh P, Tchorbanov A, Vassilev T, Erdei A, Prechl J. Progression of lupus-like disease drives the appearance of complement-activating IgG antibodies in MRL/lpr mice. Rheumatology (Oxford). 2010;49:2273-80 pubmed publisher
Crittenden S, Kimble J. Preparation and immunolabeling of Caenorhabditis elegans. Cold Spring Harb Protoc. 2009;2009:pdb.prot5216 pubmed publisher
Nishinaka T, Doi Y, Hashimoto M, Hara R, Shibata T, Harada Y, et al. Visualization of RecA filaments and DNA by fluorescence microscopy. J Biochem. 2007;141:147-56 pubmed

product information

Catalog Number :

MAB030

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-DNA Antibody, double stranded, clone BV16-13

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

DNA

Clone :

BV16-13

Conjugate :

Culture Supernatant

Isotype :

IgG2aκ

Product Description :

Anti-DNA Antibody, double stranded, clone BV16-13

Cross Reactivity :

All

Specificity :

Double stranded DNA (dsDNyA) (B-form) from human. Shows 95% reactivity to dsDNA and 28% reactivity to ssDNA. BINDING ASSAY: Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti-DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound [32P] DNA by Chernkov radiation. ANTIGEN BINDING PROFILE: Relative Binding To: Natural DNA Synthetic homopolymers dsDNA(a) ssDNA(b) poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU) 1.00 0.45 0.01 0.01 0.01 0.01 0.01 0.01 Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10 mg of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0). a) Native calf thymus DNA b) Heat-denatured calf thymus DNA

Package Size :

500 µL

Uses :

ELISA;Radioimmunoassay;Immunocytochemistry

Storage :

Maintain at 2-8°C in undiluted aliquots for up to 6 months.