PAI-1 (Ab-1) Mouse mAb (2) | EMD Millipore IM29L-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PAI-1 (Ab-1) Mouse mAb (2)

catalog :

IM29L-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, neutralization, immunoprecipitation, other

citations: 1

Published Application/Species/Sample/Dilution	Reference
other; human; loading ...; fig s1	Shah F, Stepan A, O Mahony A, Velichko S, Folias A, Houle C, et al. Mechanisms of Skin Toxicity Associated with Metabotropic Glutamate Receptor 5 Negative Allosteric Modulators. Cell Chem Biol. 2017;24:858-869.e5 pubmed publisher

product information

Catalog Number :

IM29L-100UG

Category :

Antibodies

Subcategory :

Cell Structure

Product Name :

Anti-PAI-1 (Ab-1) Mouse mAb (2)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

5054 (Hu), 18787 (Ms), 24617 (Rt)

Host Name :

Clone :

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-PAI-1 (Ab-1) Mouse mAb (2)

Cross Reactivity :

Human

Background :

Plasminogen activator inhibitor 1 (PAI-1) is a secreted glycoprotein of approximately 50 kDa, which functions as the primary regulator of uPA and tPA activity. PAI-1 and the related inhibitor, PAI-2, are members of the serine proteinase inhibitor (serpin) gene superfamily. PAI-1 is produced by a variety of cell types and is present in normal human plasma. It exists as two conformationally distinct forms, latent and active. The active form binds to uPA in a 1:1 complex resulting in the inhibition of proteolytic activity. Active PAI-1 rapidly converts to the latent form but can be stabilized in the active conformation by binding to the cell adhesion glycoprotein, vitronectin. Latent PAI-1 can be activated in vitro by exposure to denaturants such as SDS or guanidine. PAI-1 levels are increased in a number of disease states including coronary artery disease, venous thrombosis, obesity and various types of cancer.

ALT Names :

BETA MIGRATING PLAS ACTIVATOR, BETA-MIGRATING PLASMINOGEN ACTIVATOR INHIBITOR I, PAI, PAI-1, PAI1A, Pai1aa, Planh, PLANH1, PLASMINOGEN ACTIVATOR INHIBITOR 1, RATPAI1A, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1

Immunogen :

partially purified PAI-1 from conditioned medium of dexamethasone-treated HT1080 human cells

Specificity :

Recognizes both active and latent forms of the ~50 kDa PAI-1 protein.

Package Size :

100 ug

Uses :

WB, IC, IP, NEUT

Storage :

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 ug/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.