Cathepsin B (Ab-1) Mouse mAb (CA10) | EMD Millipore IM27L-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Cathepsin B (Ab-1) Mouse mAb (CA10)

catalog :

IM27L-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; loading ...; fig 5b	Hernandez Tiedra S, Fabrias G, Davila D, Salanueva I, Casas J, Montes L, et al. Dihydroceramide accumulation mediates cytotoxic autophagy of cancer cells via autolysosome destabilization. Autophagy. 2016;12:2213-2229 pubmed

product information

Catalog Number :

IM27L-100UG

Category :

Antibodies

Subcategory :

Apoptosis & Cancer

Product Name :

Anti-Cathepsin B (Ab-1) Mouse mAb (CA10)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

1508 (Hu), 13030 (Ms), 64529 (Rt)

Host Name :

Clone :

CA10

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-Cathepsin B (Ab-1) Mouse mAb (CA10)

Cross Reactivity :

Bovine, Human, Porcine

Background :

Cathepsin B (CB) is a normal lysosomal proteinase which is expressed in all cells. It is a cysteine endoproteinase and is structurally and functionally related to the papain family of proteinases. CB is synthesized as an inactive 43 kDa pro-enzyme which, by removal of a 623 amino acid propeptide, is activated to the single-chain form of 31 kDa or the two-chain form of 25 and 5 kDa subunits. Mature CB is localized in the lysosomes where it functions in protein turnover but has also been shown to be capable of degrading extracellular matrix proteins at acidic and neutral pH. Endogenous inhibitors of cysteine proteinases, the cystatins and stefins, may play a major role in regulating proteinase activity. In tumor cells and in cells exposed to mitogens, CB displays altered cellular trafficking resulting in the secretion of the 43 kDa precursor. Elevated levels of CB correlate with malignancy, suggesting that this enzyme may be a useful prognostic marker for several types of human cancers.

ALT Names :

Alpha secretase, APPS, CATHEPSIN B, Cathepsin B1, CPSB, cathepsin B

Immunogen :

purified human liver cathepsin B

Specificity :

Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B.

Package Size :

100 ug

Uses :

WB, IF, IP

Storage :

Resuspend the lyophilized antibody with 1 ml sterile PBS. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Freezing of aliquots is best for long-term storage of reconstituted product. Avoid freeze/thaw cycles.