MMP-9 (Ab-1) Mouse mAb (6-6B) | EMD Millipore IM09L-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

MMP-9 (Ab-1) Mouse mAb (6-6B)

catalog :

IM09L-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, neutralization, immunoprecipitation

product information

Catalog Number :

IM09L-100UG

Category :

Antibodies

Subcategory :

Cell Structure

Product Name :

Anti-MMP-9 (Ab-1) Mouse mAb (6-6B)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

4318 (Hu), 17395 (Ms), 81687 (Rt)

Host Name :

Clone :

6-6B

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-MMP-9 (Ab-1) Mouse mAb (6-6B)

Cross Reactivity :

Human

Background :

Matrix metalloproteinases (MMP's) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMP's share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis.MMP-9 (Gelatinase B, 92 kDa gelatinase/type IV collagenase) is secreted as a 92 kDa zymogen which is proteolytically processed to the 83 kDa active form; a 68 kDa has also been detected.

ALT Names :

AW743869, B/MMP9, CLG4B, COLLAGENASE TYPE IV, GELATINASE B, GELB, Gl 92-kda, Ngb, pro-MMP-9, matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

Immunogen :

MMP-9 from conditioned medium of PMA-stimulated HT-1080 human fibrosarcoma cells

Specificity :

Recognizes the ~92 kDa latent and the ~83 kDa active forms of human MMP-9 under non-reducing conditions, but only the latent form under reducing conditions.

Package Size :

100 ug

Uses :

WB, IP, NEUT

Storage :

Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Resuspend the antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 ug/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Freezing of aliquots is best for long-term storage of reconstituted product; repetitive freezing and thawing should be avoided.