Tau-1 Antibody, prediluted, clone PC1C6 | EMD Millipore IHCR1015-6 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Tau-1 Antibody, prediluted, clone PC1C6

catalog :

IHCR1015-6

quantity :

6 mL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PC1C6

The same clone is also sold as:

Absolute Antibody: Ab01120-23.0, Ab01120-1.32, Ab01120-2.3, Ab01120-2.0

reactivity :

human, mouse, rat, dogs

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

citations: 30

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; dogs; 1:1000; fig 4	Spitzbarth I, Lempp C, Kegler K, Ulrich R, Kalkuhl A, Deschl U, et al. Immunohistochemical and transcriptome analyses indicate complex breakdown of axonal transport mechanisms in canine distemper leukoencephalitis. Brain Behav. 2016;6:e00472 pubmed publisher
western blot; mouse; 1:1000; tbl 1	El Khoury N, Gratuze M, Petry F, Papon M, Julien C, Marcouiller F, et al. Hypothermia mediates age-dependent increase of tau phosphorylation in db/db mice. Neurobiol Dis. 2016;88:55-65 pubmed publisher
immunocytochemistry; mouse; 1:2; loading ...; fig 4a	Minoura I, Takazaki H, Ayukawa R, Saruta C, Hachikubo Y, Uchimura S, et al. Reversal of axonal growth defects in an extraocular fibrosis model by engineering the kinesin-microtubule interface. Nat Commun. 2016;7:10058 pubmed publisher
immunocytochemistry; rat; 1:1000; fig s4	Goyal U, Renvoisé B, Chang J, Blackstone C. Spastin-interacting protein NA14/SSNA1 functions in cytokinesis and axon development. PLoS ONE. 2014;9:e112428 pubmed publisher
immunohistochemistry - paraffin section; rat; loading ...; tbl 1,7	Elias P, Spector M. Treatment of penetrating brain injury in a rat model using collagen scaffolds incorporating soluble Nogo receptor. J Tissue Eng Regen Med. 2015;9:137-50 pubmed publisher
	Lee M, Liu Y, Chen C, Lu C, Lu S, Huang T, et al. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. J Cell Biol. 2020;219: pubmed publisher
	Dupraz S, Hilton B, Husch A, Santos T, Coles C, Stern S, et al. RhoA Controls Axon Extension Independent of Specification in the Developing Brain. Curr Biol. 2019;29:3874-3886.e9 pubmed publisher
	Farías G, Fréal A, Tortosa E, Stucchi R, Pan X, Portegies S, et al. Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity. Neuron. 2019;102:184-201.e8 pubmed publisher
	Zhang M, Ergin V, Lin L, Stork C, Chen L, Zheng S. Axonogenesis Is Coordinated by Neuron-Specific Alternative Splicing Programming and Splicing Regulator PTBP2. Neuron. 2019;101:690-706.e10 pubmed publisher
	Wang J, Xu S, Duan J, Yi L, Guo Y, Shi Y, et al. Invasion of white matter tracts by glioma stem cells is regulated by a NOTCH1-SOX2 positive-feedback loop. Nat Neurosci. 2019;22:91-105 pubmed publisher
	Ambrozkiewicz M, Schwark M, Kishimoto Suga M, Borisova E, Hori K, Salazar Lázaro A, et al. Polarity Acquisition in Cortical Neurons Is Driven by Synergistic Action of Sox9-Regulated Wwp1 and Wwp2 E3 Ubiquitin Ligases and Intronic miR-140. Neuron. 2018;: pubmed publisher
	Parker S, Moutal A, Cai S, Chandrasekaran S, Roman M, Koshy A, et al. High Fidelity Cryopreservation and Recovery of Primary Rodent Cortical Neurons. Eneuro. 2018;5: pubmed publisher
	Zhang P, Lu H, Peixoto R, Pines M, Ge Y, Oku S, et al. Heparan Sulfate Organizes Neuronal Synapses through Neurexin Partnerships. Cell. 2018;174:1450-1464.e23 pubmed publisher
	Bardai F, Wang L, Mutreja Y, Yenjerla M, Gamblin T, Feany M. A Conserved Cytoskeletal Signaling Cascade Mediates Neurotoxicity of FTDP-17 Tau Mutations In Vivo. J Neurosci. 2018;38:108-119 pubmed publisher
	Chang T, Chen C, Lee M, Chang Y, Lu C, Lu S, et al. Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machinery. elife. 2017;6: pubmed publisher
	Boselli M, Lee B, Robert J, Prado M, Min S, Cheng C, et al. An inhibitor of the proteasomal deubiquitinating enzyme USP14 induces tau elimination in cultured neurons. J Biol Chem. 2017;292:19209-19225 pubmed publisher
	Laurent C, Eddarkaoui S, Derisbourg M, Leboucher A, Demeyer D, Carrier S, et al. Beneficial effects of caffeine in a transgenic model of Alzheimer's disease-like tau pathology. Neurobiol Aging. 2014;35:2079-90 pubmed publisher
	Mejia L, Litterman N, Ikeuchi Y, de la Torre Ubieta L, Bennett E, Zhang C, et al. A novel Hap1-Tsc1 interaction regulates neuronal mTORC1 signaling and morphogenesis in the brain. J Neurosci. 2013;33:18015-21 pubmed publisher
	Hawkins B, Krishnamurthy S, Castillo Carranza D, Sengupta U, Prough D, Jackson G, et al. Rapid accumulation of endogenous tau oligomers in a rat model of traumatic brain injury: possible link between traumatic brain injury and sporadic tauopathies. J Biol Chem. 2013;288:17042-50 pubmed publisher
	Cirak Y, Sarsik B, Cakar B, Sen S, Simsir A, Uslu R. Predictive and prognostic values of Tau and BubR1 protein in prostate cancer and their relationship to the Gleason score. Med Oncol. 2013;30:526 pubmed publisher
	Yaka C, Björk P, Schönberg T, Erlandsson A. A novel in vitro injury model based on microcontact printing demonstrates negative effects of hydrogen peroxide on axonal regeneration both in absence and presence of glia. J Neurotrauma. 2013;30:392-402 pubmed publisher
	Magnaudeix A, Wilson C, Page G, Bauvy C, Codogno P, Leveque P, et al. PP2A blockade inhibits autophagy and causes intraneuronal accumulation of ubiquitinated proteins. Neurobiol Aging. 2013;34:770-90 pubmed publisher
	Orozco D, Tahirovic S, Rentzsch K, Schwenk B, Haass C, Edbauer D. Loss of fused in sarcoma (FUS) promotes pathological Tau splicing. EMBO Rep. 2012;13:759-64 pubmed publisher
	An S, Tsai C, Ronecker J, Bayly A, Herzog E. Spatiotemporal distribution of vasoactive intestinal polypeptide receptor 2 in mouse suprachiasmatic nucleus. J Comp Neurol. 2012;520:2730-41 pubmed publisher
	Alvarez G, Aldudo J, Alonso M, Santana S, Valdivieso F. Herpes simplex virus type 1 induces nuclear accumulation of hyperphosphorylated tau in neuronal cells. J Neurosci Res. 2012;90:1020-9 pubmed publisher
	Millet L, Collens M, Perry G, Bashir R. Pattern analysis and spatial distribution of neurons in culture. Integr Biol (Camb). 2011;3:1167-78 pubmed publisher
	Neukirchen D, Bradke F. Cytoplasmic linker proteins regulate neuronal polarization through microtubule and growth cone dynamics. J Neurosci. 2011;31:1528-38 pubmed publisher
	Tahirovic S, Hellal F, Neukirchen D, Hindges R, Garvalov B, FLYNN K, et al. Rac1 regulates neuronal polarization through the WAVE complex. J Neurosci. 2010;30:6930-43 pubmed publisher
	Garvalov B, FLYNN K, Neukirchen D, Meyn L, Teusch N, Wu X, et al. Cdc42 regulates cofilin during the establishment of neuronal polarity. J Neurosci. 2007;27:13117-29 pubmed
	Togashi H, Miyoshi J, Honda T, Sakisaka T, Takai Y, Takeichi M. Interneurite affinity is regulated by heterophilic nectin interactions in concert with the cadherin machinery. J Cell Biol. 2006;174:141-51 pubmed

product information

Catalog Number :

IHCR1015-6

Subcategory :

Neuroscience

Product Name :

Anti-Tau-1 Antibody, prediluted, clone PC1C6

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P10636

Host Name :

Mouse

Antigen :

Tau-1

Clone :

PC1C6

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-Tau-1 Antibody, prediluted, clone PC1C6

Cross Reactivity :

Bovine;Human;Rat

Immunogen :

Purified denatured bovine microtubule associated proteins.

Specificity :

Cellular and subcellular localization: In situ, anti-tau-1 has a stringent specificity for the axons of neurons. The antibody does not stain the cell bodies or dendrites of neurons, nor does it stain any other cell type (4). However, this in vivo intracellular specificity is not maintained in culture: anti-tau-1 stains the axon, cell bodies, and dendrites of rat hippocampal neurons grown in culture (5). The specificity of anti-tau-1 was originally thought to represent the restricted expression of tau to axons. Later studies revealed that this specificity is dependant on the state of phosphorylation. In dephosphorylated samples (samples treated with alkaline phosphatase) anti-tau-1 stains astrocytes, perineuronal glial cells, and the axons, cell bodies and dendrites of neurons, while in untreated samples, anti-tau-1 stains only axons (6). (The epitope recognized by anti-tau-1 is probably at or near a phosphorylated site.). Anti-tau-1 binds to all known electrophoretic species of tau in human, rat and bovine brain (one-dimensional SDS-PAGE). However there is some unphosphorylated bias with clone PC1C6 as it seem to recognize only dephosphorylated serine sites at 195, 198, 199, and 202 {Szendrei, et al 1993; http://www.ncbi.nlm.nih.gov/entrez/query.fcgi-cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7680727}. Also see Billingsley & Kincaid, 1997 Biochem J 323:577-591 for additional mapping information on PC1C6.

Package Size :

6 mL

Uses :

Immunohistochemistry (Paraffin)

Storage :

Maintain at 2-8°C. Refer to vial for expiration dating.