EGFR (Ab-1) Mouse mAb (528) | EMD Millipore GR01L-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

EGFR (Ab-1) Mouse mAb (528)

catalog :

GR01L-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry, neutralization, immunoprecipitation, flow cytometry

citations: 1

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; loading ...; fig 1d	Francavilla C, Papetti M, Rigbolt K, Pedersen A, Sigurdsson J, Cazzamali G, et al. Multilayered proteomics reveals molecular switches dictating ligand-dependent EGFR trafficking. Nat Struct Mol Biol. 2016;23:608-18 pubmed publisher

product information

Catalog Number :

GR01L-100UG

Category :

Antibodies

Subcategory :

Cell Signaling

Product Name :

Anti-EGFR (Ab-1) Mouse mAb (528)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

1956 (Hu), 13649 (Ms), 24329 (Rt)

Host Name :

Clone :

528

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-EGFR (Ab-1) Mouse mAb (528)

Cross Reactivity :

Human

Background :

Epidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF or transforming growth factor a to the receptor activates a tyrosine-specific protein kinase intrinsic to the EGF-receptor protein. The phosphotyrosine content of target cells increases rapidly and the EGF-receptor is phosphorylated on tyrosine residues.

ALT Names :

EGF RECEPTOR, EGF-TK, EGFR1, EGFRec, ER2, ERBB, ERBB1, Errp, HER1, MENA, PIG61, wa-2, Wa5, epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)

Immunogen :

partially purified, human EGFR from human A431 cells

Specificity :

Recognizes the ~170 kDa EGFR protein.

Package Size :

100 ug

Uses :

FC, IH, IF, IP, NEUT, IH(P)

Storage :

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 ug/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.