This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Milli-Mark™ Anti-Rac1-FITC Antibody, clone 23A8
catalog :
FCMAB330F
quantity :
100 tests
clonality :
monoclonal
host :
mouse
conjugate :
FITC
clone name :
23A8

The same clone is also sold as:
reactivity :
human, mouse
application :
flow cytometry
citations: 65
Reference
Menhofer M, Kubisch R, Schreiner L, Zorn M, Foerster F, Mueller R, et al. The actin targeting compound Chondramide inhibits breast cancer metastasis via reduction of cellular contractility. PLoS ONE. 2014;9:e112542 pubmed publisher
Bancroft T, Bouaouina M, Roberts S, Lee M, Calderwood D, Schwartz M, et al. Up-regulation of thrombospondin-2 in Akt1-null mice contributes to compromised tissue repair due to abnormalities in fibroblast function. J Biol Chem. 2015;290:409-22 pubmed publisher
Larson Casey J, Murthy S, Ryan A, Carter A. Modulation of the mevalonate pathway by akt regulates macrophage survival and development of pulmonary fibrosis. J Biol Chem. 2014;289:36204-19 pubmed publisher
Watanabe M, Terasawa M, Miyano K, Yanagihara T, Uruno T, Sanematsu F, et al. DOCK2 and DOCK5 act additively in neutrophils to regulate chemotaxis, superoxide production, and extracellular trap formation. J Immunol. 2014;193:5660-7 pubmed publisher
Gray E, Petsalaki E, James D, Bagshaw R, Stacey M, Rocks O, et al. Src homology 2 domain containing protein 5 (SH2D5) binds the breakpoint cluster region protein, BCR, and regulates levels of Rac1-GTP. J Biol Chem. 2014;289:35397-408 pubmed publisher
Köster M, Dell Orco D, Koch K. The interaction network of rhodopsin involving the heterotrimeric G-protein transducin and the monomeric GTPase Rac1 is determined by distinct binding processes. FEBS J. 2014;281:5175-85 pubmed publisher
Menhofer M, Bartel D, Liebl J, Kubisch R, Busse J, Wagner E, et al. In vitro and in vivo characterization of the actin polymerizing compound chondramide as an angiogenic inhibitor. Cardiovasc Res. 2014;104:303-14 pubmed publisher
Song H, Bush R, Vijayasarathy C, Fariss R, Kjellstrom S, Sieving P. Transgenic expression of constitutively active RAC1 disrupts mouse rod morphogenesis. Invest Ophthalmol Vis Sci. 2014;55:2659-68 pubmed publisher
Gungor B, Gombos I, Crul T, Ayaydin F, Szabo L, Torok Z, et al. Rac1 participates in thermally induced alterations of the cytoskeleton, cell morphology and lipid rafts, and regulates the expression of heat shock proteins in B16F10 melanoma cells. PLoS ONE. 2014;9:e89136 pubmed publisher
Rahman A, Davis B, Lövdahl C, Hanumaiah V, Feil R, Brakebusch C, et al. The small GTPase Rac1 is required for smooth muscle contraction. J Physiol. 2014;592:915-26 pubmed publisher
Cheung H, Dunbar C, Mórotz G, Cheng W, Chan H, Miller C, et al. FE65 interacts with ADP-ribosylation factor 6 to promote neurite outgrowth. FASEB J. 2014;28:337-49 pubmed publisher
Itakura A, Aslan J, Kusanto B, Phillips K, Porter J, Newton P, et al. p21-Activated kinase (PAK) regulates cytoskeletal reorganization and directional migration in human neutrophils. PLoS ONE. 2013;8:e73063 pubmed publisher
Tang X, Jin R, Qu G, Wang X, Li Z, Yuan Z, et al. GPR116, an adhesion G-protein-coupled receptor, promotes breast cancer metastasis via the G?q-p63RhoGEF-Rho GTPase pathway. Cancer Res. 2013;73:6206-18 pubmed publisher
Favaro P, Traina F, Machado Neto J, Lazarini M, Lopes M, Pereira J, et al. FMNL1 promotes proliferation and migration of leukemia cells. J Leukoc Biol. 2013;94:503-12 pubmed publisher
Aslan J, Baker S, Loren C, Haley K, Itakura A, Pang J, et al. The PAK system links Rho GTPase signaling to thrombin-mediated platelet activation. Am J Physiol Cell Physiol. 2013;305:C519-28 pubmed publisher
Sakai Y, Tanaka Y, Yanagihara T, Watanabe M, Duan X, Terasawa M, et al. The Rac activator DOCK2 regulates natural killer cell-mediated cytotoxicity in mice through the lytic synapse formation. Blood. 2013;122:386-93 pubmed publisher
Ahn J, Truesdell P, Meens J, Kadish C, Yang X, Boag A, et al. Fer protein-tyrosine kinase promotes lung adenocarcinoma cell invasion and tumor metastasis. Mol Cancer Res. 2013;11:952-63 pubmed publisher
Duman J, Tzeng C, Tu Y, Munjal T, Schwechter B, Ho T, et al. The adhesion-GPCR BAI1 regulates synaptogenesis by controlling the recruitment of the Par3/Tiam1 polarity complex to synaptic sites. J Neurosci. 2013;33:6964-78 pubmed publisher
Jun J, Yang M, Chen H, Chakraborty A, Roose J. Activation of extracellular signal-regulated kinase but not of p38 mitogen-activated protein kinase pathways in lymphocytes requires allosteric activation of SOS. Mol Cell Biol. 2013;33:2470-84 pubmed publisher
Gerhard R, Frenzel E, Goy S, Olling A. Cellular uptake of Clostridium difficile TcdA and truncated TcdA lacking the receptor binding domain. J Med Microbiol. 2013;62:1414-22 pubmed publisher
Lopez Haber C, Kazanietz M. Cucurbitacin I inhibits Rac1 activation in breast cancer cells by a reactive oxygen species-mediated mechanism and independently of Janus tyrosine kinase 2 and P-Rex1. Mol Pharmacol. 2013;83:1141-54 pubmed publisher
Sanematsu F, Nishikimi A, Watanabe M, Hongu T, Tanaka Y, Kanaho Y, et al. Phosphatidic acid-dependent recruitment and function of the Rac activator DOCK1 during dorsal ruffle formation. J Biol Chem. 2013;288:8092-100 pubmed publisher
Diesel B, Hoppstädter J, Hachenthal N, Zarbock R, Cavelius C, Wahl B, et al. Activation of Rac1 GTPase by nanoparticulate structures in human macrophages. Eur J Pharm Biopharm. 2013;84:315-24 pubmed publisher
Chen P, Jian X, Yoon H, Randazzo P. ARAP2 signals through Arf6 and Rac1 to control focal adhesion morphology. J Biol Chem. 2013;288:5849-60 pubmed publisher
Cajanek L, Ganji R, Henriques Oliveira C, Theofilopoulos S, Konik P, Bryja V, et al. Tiam1 regulates the Wnt/Dvl/Rac1 signaling pathway and the differentiation of midbrain dopaminergic neurons. Mol Cell Biol. 2013;33:59-70 pubmed publisher
Meyer G, Leipprandt J, Xie J, Aupperlee M, Haslam S. A potential role of progestin-induced laminin-5/?6-integrin signaling in the formation of side branches in the mammary gland. Endocrinology. 2012;153:4990-5001 pubmed
López Gómez A, Cano V, Moranta D, Morey P, Garcia del Portillo F, Bengoechea J, et al. Host cell kinases, ?5 and ?1 integrins, and Rac1 signalling on the microtubule cytoskeleton are important for non-typable Haemophilus influenzae invasion of respiratory epithelial cells. Microbiology. 2012;158:2384-98 pubmed publisher
Guttenberg G, Hornei S, Jank T, Schwan C, Lu W, Einsle O, et al. Molecular characteristics of Clostridium perfringens TpeL toxin and consequences of mono-O-GlcNAcylation of Ras in living cells. J Biol Chem. 2012;287:24929-40 pubmed publisher
Medlin M, Taylor J, Mack C. Quantifying sphingosine-1-phosphate-dependent activation of the RhoGTPases. Methods Mol Biol. 2012;874:89-97 pubmed publisher
Ridgway L, Wetzel M, Ngo J, Erdreich Epstein A, Marchetti D. Heparanase-induced GEF-H1 signaling regulates the cytoskeletal dynamics of brain metastatic breast cancer cells. Mol Cancer Res. 2012;10:689-702 pubmed publisher
Kawarazaki W, Nagase M, Yoshida S, Takeuchi M, Ishizawa K, Ayuzawa N, et al. Angiotensin II- and salt-induced kidney injury through Rac1-mediated mineralocorticoid receptor activation. J Am Soc Nephrol. 2012;23:997-1007 pubmed publisher
Mijouin L, Rosselin M, Bottreau E, Pizarro Cerda J, Cossart P, Velge P, et al. Salmonella enteritidis Rck-mediated invasion requires activation of Rac1, which is dependent on the class I PI 3-kinases-Akt signaling pathway. FASEB J. 2012;26:1569-81 pubmed publisher
Yamagata K, Li X, Ikegaki S, Oneyama C, Okada M, Nishita M, et al. Dissection of Wnt5a-Ror2 signaling leading to matrix metalloproteinase (MMP-13) expression. J Biol Chem. 2012;287:1588-99 pubmed publisher
Watanabe T, Urano E, Miyauchi K, Ichikawa R, Hamatake M, Misawa N, et al. The hematopoietic cell-specific Rho GTPase inhibitor ARHGDIB/D4GDI limits HIV type 1 replication. AIDS Res Hum Retroviruses. 2012;28:913-22 pubmed publisher
Zeiser J, Klodmann J, Braun H, Gerhard R, Just I, Pich A. Effects of Clostridium difficile Toxin A on the proteome of colonocytes studied by differential 2D electrophoresis. J Proteomics. 2011;75:469-79 pubmed publisher
Hale C, Dietz K, Varela J, Wood C, Zirlin B, Leverich L, et al. Essential role for vav Guanine nucleotide exchange factors in brain-derived neurotrophic factor-induced dendritic spine growth and synapse plasticity. J Neurosci. 2011;31:12426-36 pubmed publisher
Aslan J, Tormoen G, Loren C, Pang J, McCarty O. S6K1 and mTOR regulate Rac1-driven platelet activation and aggregation. Blood. 2011;118:3129-36 pubmed publisher
Lacy P, Willetts L, Kim J, Lo A, Lam B, Maclean E, et al. Agonist activation of f-actin-mediated eosinophil shape change and mediator release is dependent on Rac2. Int Arch Allergy Immunol. 2011;156:137-47 pubmed publisher
Nikolic D, Lehmann M, Felts R, Garcia E, Blanchet F, Subramaniam S, et al. HIV-1 activates Cdc42 and induces membrane extensions in immature dendritic cells to facilitate cell-to-cell virus propagation. Blood. 2011;118:4841-52 pubmed publisher
Polizio A, Chinchilla P, Chen X, Kim S, Manning D, Riobo N. Heterotrimeric Gi proteins link Hedgehog signaling to activation of Rho small GTPases to promote fibroblast migration. J Biol Chem. 2011;286:19589-96 pubmed publisher
Chan D, Citro A, Cordy J, Shen G, Wolozin B. Rac1 protein rescues neurite retraction caused by G2019S leucine-rich repeat kinase 2 (LRRK2). J Biol Chem. 2011;286:16140-9 pubmed publisher
Park H, Cox D. Syk regulates multiple signaling pathways leading to CX3CL1 chemotaxis in macrophages. J Biol Chem. 2011;286:14762-9 pubmed publisher
Mains R, Kiraly D, Eipper Mains J, Ma X, Eipper B. Kalrn promoter usage and isoform expression respond to chronic cocaine exposure. BMC Neurosci. 2011;12:20 pubmed publisher
Infante E, Heasman S, Ridley A. Statins inhibit T-acute lymphoblastic leukemia cell adhesion and migration through Rap1b. J Leukoc Biol. 2011;89:577-86 pubmed publisher
Petrusca D, Gu Y, Adamowicz J, Rush N, Hubbard W, Smith P, et al. Sphingolipid-mediated inhibition of apoptotic cell clearance by alveolar macrophages. J Biol Chem. 2010;285:40322-32 pubmed publisher
Heasman S, Carlin L, Cox S, Ng T, Ridley A. Coordinated RhoA signaling at the leading edge and uropod is required for T cell transendothelial migration. J Cell Biol. 2010;190:553-63 pubmed publisher
Papatheodorou P, Zamboglou C, Genisyuerek S, Guttenberg G, Aktories K. Clostridial glucosylating toxins enter cells via clathrin-mediated endocytosis. PLoS ONE. 2010;5:e10673 pubmed publisher
Tahirovic S, Hellal F, Neukirchen D, Hindges R, Garvalov B, FLYNN K, et al. Rac1 regulates neuronal polarization through the WAVE complex. J Neurosci. 2010;30:6930-43 pubmed publisher
van Duijn T, Anthony E, Hensbergen P, Deelder A, Hordijk P. Rac1 recruits the adapter protein CMS/CD2AP to cell-cell contacts. J Biol Chem. 2010;285:20137-46 pubmed publisher
Sitrin R, Sassanella T, Petty H. An obligate role for membrane-associated neutral sphingomyelinase activity in orienting chemotactic migration of human neutrophils. Am J Respir Cell Mol Biol. 2011;44:205-12 pubmed publisher
Gotoh K, Tanaka Y, Nishikimi A, Nakamura R, Yamada H, Maeda N, et al. Selective control of type I IFN induction by the Rac activator DOCK2 during TLR-mediated plasmacytoid dendritic cell activation. J Exp Med. 2010;207:721-30 pubmed publisher
Gopal S, Bober A, Whiteford J, Multhaupt H, Yoneda A, Couchman J. Heparan sulfate chain valency controls syndecan-4 function in cell adhesion. J Biol Chem. 2010;285:14247-58 pubmed publisher
Deng K, Gao Y, Cao Z, Graziani E, Wood A, Doherty P, et al. Overcoming amino-Nogo-induced inhibition of cell spreading and neurite outgrowth by 12-O-tetradecanoylphorbol-13-acetate-type tumor promoters. J Biol Chem. 2010;285:6425-33 pubmed publisher
Chatterjee A, Wang L, Armstrong D, Rossie S. Activated Rac1 GTPase translocates protein phosphatase 5 to the cell membrane and stimulates phosphatase activity in vitro. J Biol Chem. 2010;285:3872-82 pubmed publisher
Antoku S, Mayer B. Distinct roles for Crk adaptor isoforms in actin reorganization induced by extracellular signals. J Cell Sci. 2009;122:4228-38 pubmed publisher
Xie Y, Wolff D, Wei T, Wang B, Deng C, Kirui J, et al. Breast cancer migration and invasion depend on proteasome degradation of regulator of G-protein signaling 4. Cancer Res. 2009;69:5743-51 pubmed publisher
Haruta M, Bush R, Kjellstrom S, Vijayasarathy C, Zeng Y, Le Y, et al. Depleting Rac1 in mouse rod photoreceptors protects them from photo-oxidative stress without affecting their structure or function. Proc Natl Acad Sci U S A. 2009;106:9397-402 pubmed publisher
Qin J, Xie Y, Wang B, Hoshino M, Wolff D, Zhao J, et al. Upregulation of PIP3-dependent Rac exchanger 1 (P-Rex1) promotes prostate cancer metastasis. Oncogene. 2009;28:1853-63 pubmed publisher
Moissoglu K, McRoberts K, Meier J, Theodorescu D, Schwartz M. Rho GDP dissociation inhibitor 2 suppresses metastasis via unconventional regulation of RhoGTPases. Cancer Res. 2009;69:2838-44 pubmed publisher
Marshall M, Anilkumar N, Layland J, Walker S, Kentish J, Shah A, et al. Protein phosphatase 2A contributes to the cardiac dysfunction induced by endotoxemia. Cardiovasc Res. 2009;82:67-76 pubmed publisher
Ma X, Kiraly D, Gaier E, Wang Y, Kim E, Levine E, et al. Kalirin-7 is required for synaptic structure and function. J Neurosci. 2008;28:12368-82 pubmed publisher
Liebig T, Erasmus J, Kalaji R, Davies D, Loirand G, Ridley A, et al. RhoE Is required for keratinocyte differentiation and stratification. Mol Biol Cell. 2009;20:452-63 pubmed publisher
Garvalov B, FLYNN K, Neukirchen D, Meyn L, Teusch N, Wu X, et al. Cdc42 regulates cofilin during the establishment of neuronal polarity. J Neurosci. 2007;27:13117-29 pubmed
Fotiadou P, Takahashi C, Rajabi H, Ewen M. Wild-type NRas and KRas perform distinct functions during transformation. Mol Cell Biol. 2007;27:6742-55 pubmed
Egerer M, Giesemann T, Jank T, Satchell K, Aktories K. Auto-catalytic cleavage of Clostridium difficile toxins A and B depends on cysteine protease activity. J Biol Chem. 2007;282:25314-21 pubmed
product information
Catalog Number :
FCMAB330F
Subcategory :
Cell Signaling
Product Name :
Milli-Mark™ Anti-Rac1-FITC Antibody, clone 23A8
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P63000
Host Name :
Mouse
Antigen :
Rac1-FITC
Clone :
23A8
Conjugate :
FITC
Isotype :
IgG2b
Product Description :
Milli-Mark™ Anti-Rac1-FITC Antibody, clone 23A8
Cross Reactivity :
Rat;Mouse;Human
Background :
Rac1 is a member of a RhoGTPase subfamily (Rac1-Rac4) that transduces extracellular signals from G-coupled protein receptors (GPCR), integrins and growth factor receptors to effector molecules that modulate multiple signaling pathways. While Rac1 supports the activation of MAPkinase pathways and gene activation, it’s most important functions include the regulation of cytoskeletal structures. Together with Rho (regulator of stress fibers) and Cdc42 (regulator of filopodia), Rac modulates the formation of focal adhesion (FA) complexes; membrane ruffles and lamellipodia that contribute to important cell functions related to cell attachment and movement.
Immunogen :
Recombinant protein containing the full length human Rac1.
Specificity :
Antibody recognizes Rac1. Slight Rac2 cross-reactivity.
Package Size :
100 tests
Uses :
Flow Cytometry
Storage :
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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