Milli-Mark™ Anti-ubiquityl-Histone H2A-PE Antibody, clone E6C5 | EMD Millipore FCMAB308P product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Milli-Mark™ Anti-ubiquityl-Histone H2A-PE Antibody, clone E6C5

catalog :

FCMAB308P

quantity :

100 tests

clonality :

monoclonal

host :

mouse

conjugate :

clone name :

E6C5

reactivity :

human

application :

flow cytometry

citations: 17

Reference
Inagaki T, Iwasaki S, Matsumura Y, Kawamura T, Tanaka T, Abe Y, et al. The FBXL10/KDM2B scaffolding protein associates with novel polycomb repressive complex-1 to regulate adipogenesis. J Biol Chem. 2015;290:4163-77 pubmed publisher
Fereres S, SimÃ³n R, Mohd Sarip A, Verrijzer C, Busturia A. dRYBP counteracts chromatin-dependent activation and repression of transcription. PLoS ONE. 2014;9:e113255 pubmed publisher
Sharma N, Zhu Q, Wani G, He J, Wang Q, Wani A. USP3 counteracts RNF168 via deubiquitinating H2A and ?H2AX at lysine 13 and 15. Cell Cycle. 2014;13:106-14 pubmed publisher
Sy S, Jiang J, O W, Deng Y, Huen M. The ubiquitin specific protease USP34 promotes ubiquitin signaling at DNA double-strand breaks. Nucleic Acids Res. 2013;41:8572-80 pubmed publisher
Buchsbaum S, Bercovich B, Ziv T, Ciechanover A. Modification of the inflammatory mediator LRRFIP2 by the ubiquitin-like protein FAT10 inhibits its activity during cellular response to LPS. Biochem Biophys Res Commun. 2012;428:11-6 pubmed publisher
Noda A, Hirai Y, Hamasaki K, Mitani H, Nakamura N, Kodama Y. Unrepairable DNA double-strand breaks that are generated by ionising radiation determine the fate of normal human cells. J Cell Sci. 2012;125:5280-7 pubmed publisher
Endoh M, Endo T, Endoh T, Isono K, Sharif J, Ohara O, et al. Histone H2A mono-ubiquitination is a crucial step to mediate PRC1-dependent repression of developmental genes to maintain ES cell identity. PLoS Genet. 2012;8:e1002774 pubmed publisher
Oguro H, Yuan J, Tanaka S, Miyagi S, Mochizuki Kashio M, Ichikawa H, et al. Lethal myelofibrosis induced by Bmi1-deficient hematopoietic cells unveils a tumor suppressor function of the polycomb group genes. J Exp Med. 2012;209:445-54 pubmed publisher
Mochizuki Kashio M, Mishima Y, Miyagi S, Negishi M, Saraya A, Konuma T, et al. Dependency on the polycomb gene Ezh2 distinguishes fetal from adult hematopoietic stem cells. Blood. 2011;118:6553-61 pubmed publisher
Lang G, Bonnet J, Umlauf D, Karmodiya K, Koffler J, Stierlé M, et al. The tightly controlled deubiquitination activity of the human SAGA complex differentially modifies distinct gene regulatory elements. Mol Cell Biol. 2011;31:3734-44 pubmed publisher
Sinnar S, Small C, Evanoff R, Reinholdt L, Griswold M, Kopito R, et al. Altered testicular gene expression patterns in mice lacking the polyubiquitin gene Ubb. Mol Reprod Dev. 2011;78:415-25 pubmed publisher
Yuan J, Takeuchi M, Negishi M, Oguro H, Ichikawa H, Iwama A. Bmi1 is essential for leukemic reprogramming of myeloid progenitor cells. Leukemia. 2011;25:1335-43 pubmed publisher
Eskeland R, Freyer E, Leeb M, Wutz A, Bickmore W. Histone acetylation and the maintenance of chromatin compaction by Polycomb repressive complexes. Cold Spring Harb Symp Quant Biol. 2010;75:71-8 pubmed publisher
Joo H, Jones A, Yang C, Zhai L, Smith A, Zhang Z, et al. Regulation of histone H2A and H2B deubiquitination and Xenopus development by USP12 and USP46. J Biol Chem. 2011;286:7190-201 pubmed publisher
Li X, Corsa C, Pan P, Wu L, Ferguson D, Yu X, et al. MOF and H4 K16 acetylation play important roles in DNA damage repair by modulating recruitment of DNA damage repair protein Mdc1. Mol Cell Biol. 2010;30:5335-47 pubmed publisher
Feng L, Wang J, Chen J. The Lys63-specific deubiquitinating enzyme BRCC36 is regulated by two scaffold proteins localizing in different subcellular compartments. J Biol Chem. 2010;285:30982-8 pubmed publisher
Baumann C, De La Fuente R. ATRX marks the inactive X chromosome (Xi) in somatic cells and during imprinted X chromosome inactivation in trophoblast stem cells. Chromosoma. 2009;118:209-22 pubmed publisher

product information

Catalog Number :

FCMAB308P

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Milli-Mark™ Anti-ubiquityl-Histone H2A-PE Antibody, clone E6C5

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q96QV6

Host Name :

Mouse

Antigen :

ubiquityl-Histone H2A-PE

Clone :

E6C5

Conjugate :

Phycoerythrin

Isotype :

IgM

Product Description :

Milli-Mark™ Anti-ubiquityl-Histone H2A-PE Antibody, clone E6C5

Cross Reactivity :

Human

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histones are modified post-translationally by acetylation, phosphorylation, methylation, and ubiquitination and these modifications regulate DNA transcription, repair, recombination, and replication. Ubiquitylation usually targets the substrate for degradation, although histones H2A and H2B are actually stabilized by a single ubiquitin conjugation. Histone ubiquitination has been correlated with DNA repair and transcription, cellular differentiation, cell cycle regulation, spermatogenesis, protein trafficking, and response to stress. Histone H2A is monoubiquitinated at Lys119 by the PRC-1L complex (Polycomb repressive complex 1-like), which includes Ring1, Ring2, Bmi1 and HPH2. Ubiquitinated H2A normally represents about 15% of H2A, but this value can be as high as 50% in active chromatin. Ubiquitinyl histone H2A has also been associated with transcriptional silencing of large chromatin regions and linked to Polycomb silencing so the function of ubiquitinated H2A remains undefined.

ALT Names :

Histone H2A-GL101;Histone H2A/q

Immunogen :

AMA (human epithelial amnion) cell residual nuclear pellet portions

Specificity :

Antibody recognizes Human Ubiquityl Histone H2A.

Package Size :

100 tests

Uses :

Flow Cytometry

Storage :

Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.