Milli-Mark™ Anti-Cortactin (p80/85)-FITC Antibody, clone 4F11 | EMD Millipore FCMAB272F product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Milli-Mark™ Anti-Cortactin (p80/85)-FITC Antibody, clone 4F11

catalog :

FCMAB272F

quantity :

100 tests

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

4F11

The same clone is also sold as:

Abcam: ab33333

reactivity :

human

application :

flow cytometry

citations: 9

Reference
Payne L, Eves R, Jia L, Mak A. p53 Down regulates PDGF-induced formation of circular dorsal ruffles in rat aortic smooth muscle cells. PLoS ONE. 2014;9:e108257 pubmed publisher
Grass G, Tolliver L, Bratoeva M, Toole B. CD147, CD44, and the epidermal growth factor receptor (EGFR) signaling pathway cooperate to regulate breast epithelial cell invasiveness. J Biol Chem. 2013;288:26089-104 pubmed publisher
Gal J, Chen J, Barnett K, Yang L, Brumley E, Zhu H. HDAC6 regulates mutant SOD1 aggregation through two SMIR motifs and tubulin acetylation. J Biol Chem. 2013;288:15035-45 pubmed publisher
Juin A, Planus E, Guillemot F, Horáková P, Albiges Rizo C, Genot E, et al. Extracellular matrix rigidity controls podosome induction in microvascular endothelial cells. Biol Cell. 2013;105:46-57 pubmed publisher
Meiler E, Nieto Pelegrin E, Martinez Quiles N. Cortactin tyrosine phosphorylation promotes its deacetylation and inhibits cell spreading. PLoS ONE. 2012;7:e33662 pubmed publisher
Vega F, Fruhwirth G, Ng T, Ridley A. RhoA and RhoC have distinct roles in migration and invasion by acting through different targets. J Cell Biol. 2011;193:655-65 pubmed publisher
Bañón Rodríguez I, Monypenny J, Ragazzini C, Franco A, Calle Y, Jones G, et al. The cortactin-binding domain of WIP is essential for podosome formation and extracellular matrix degradation by murine dendritic cells. Eur J Cell Biol. 2011;90:213-23 pubmed publisher
Tahirovic S, Hellal F, Neukirchen D, Hindges R, Garvalov B, FLYNN K, et al. Rac1 regulates neuronal polarization through the WAVE complex. J Neurosci. 2010;30:6930-43 pubmed publisher
Liang Y, Niederstrasser H, Edwards M, Jackson C, Cooper J. Distinct roles for CARMIL isoforms in cell migration. Mol Biol Cell. 2009;20:5290-305 pubmed publisher

product information

Catalog Number :

FCMAB272F

Subcategory :

Cell Structure

Product Name :

Milli-Mark™ Anti-Cortactin (p80/85)-FITC Antibody, clone 4F11

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q14247

Host Name :

Mouse

Antigen :

Cortactin (p80/85)-FITC

Clone :

4F11

Conjugate :

FITC

Isotype :

IgG1

Product Description :

Milli-Mark™ Anti-Cortactin (p80/85)-FITC Antibody, clone 4F11

Cross Reactivity :

Human

Background :

Cortactin is present as a p80 and p85 kDa protein in many cell types. The protein was first identified as being a major substrate of the tyrosine kinase pp60SRC. The main structural feature of the protein is that at the N-terminus there are five and a half 37-amino acid repeats, these appear to be responsible for the actin-binding. This repeat region occupies about a third of the protein. The repeat region is followed by a helical region and then a proline rich region and finally at the C-terminus, an SH3 domain. The proline rich domain is thought to bind the SH2 domain of v-src the viral version of the normal c-src known to phosphorylate cortactin. Cortactin bundles microfilaments and this bundling is inhibited by tyrosine phosphorylation. One pathway that activates the pathway leading to tyrosine phosphorylation of cortactin results from a cell adhesion molecule in brain micro-vessel endothelial cells, another is the growth factor, but presumably the very many mechanisms for pp60src activation would also result in cortactin phosphorylation and subsequent loss of actin binding.

ALT Names :

Src substrate cortactin;Amplaxin;Oncogene EMS1

Immunogen :

Mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src

Specificity :

Antibody recognizes Cortactin

Package Size :

100 tests

Uses :

Flow Cytometry

Storage :

Maintain refrigerated at 2-8°C protected from light for up to 6 months from date of receipt.