Milli-Mark™Anti-Src-FITC Antibody, clone GD11 | EMD Millipore FCMAB253F product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Milli-Mark™Anti-Src-FITC Antibody, clone GD11

catalog :

FCMAB253F

quantity :

100 tests

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

GD11

reactivity :

human, mouse

application :

flow cytometry

citations: 41

Reference
Flierl U, Nero T, Lim B, Arthur J, Yao Y, Jung S, et al. Phosphorothioate backbone modifications of nucleotide-based drugs are potent platelet activators. J Exp Med. 2015;212:129-37 pubmed publisher
Bach C, Murray R, Owen D, Gaus K, O Neill G. Tropomyosin Tm5NM1 spatially restricts src kinase activity through perturbation of Rab11 vesicle trafficking. Mol Cell Biol. 2014;34:4436-46 pubmed publisher
Fan P, Agboke F, Cunliffe H, Ramos P, Jordan V. A molecular model for the mechanism of acquired tamoxifen resistance in breast cancer. Eur J Cancer. 2014;50:2866-76 pubmed publisher
Morinaga T, Abe K, Nakayama Y, Yamaguchi N, Yamaguchi N. Activation of Lyn tyrosine kinase through decreased membrane cholesterol levels during a change in its membrane distribution upon cell detachment. J Biol Chem. 2014;289:26327-43 pubmed publisher
Bradbury P, Bach C, Paul A, O Neill G. Src kinase determines the dynamic exchange of the docking protein NEDD9 (neural precursor cell expressed developmentally down-regulated gene 9) at focal adhesions. J Biol Chem. 2014;289:24792-800 pubmed publisher
Fukumoto Y, Miura T, Morii M, Kubota S, Honda T, Kubota S, et al. v-Src inhibits the interaction between Rad17 and Rad9 and induces replication fork collapse. Biochem Biophys Res Commun. 2014;450:885-90 pubmed publisher
Spring K, Lapointe L, Caron C, Langlois S, Royal I. Phosphorylation of DEP-1/PTPRJ on threonine 1318 regulates Src activation and endothelial cell permeability induced by vascular endothelial growth factor. Cell Signal. 2014;26:1283-93 pubmed publisher
Stutz E, Gautschi O, Fey M, Gugger M, Tschan M, Rothschild S. Crizotinib inhibits migration and expression of ID1 in MET-positive lung cancer cells: implications for MET targeting in oncology. Future Oncol. 2014;10:211-7 pubmed publisher
Ambati S, Lopes E, Kosugi K, Mony U, Zehir A, Shah S, et al. Pre-clinical efficacy of PU-H71, a novel HSP90 inhibitor, alone and in combination with bortezomib in Ewing sarcoma. Mol Oncol. 2014;8:323-36 pubmed publisher
Destaing O, Ferguson S, Grichine A, Oddou C, De Camilli P, Albiges Rizo C, et al. Essential function of dynamin in the invasive properties and actin architecture of v-Src induced podosomes/invadosomes. PLoS ONE. 2013;8:e77956 pubmed publisher
Bijian K, Lougheed C, Su J, Xu B, Yu H, Wu J, et al. Targeting focal adhesion turnover in invasive breast cancer cells by the purine derivative reversine. Br J Cancer. 2013;109:2810-8 pubmed publisher
Kubota S, Fukumoto Y, Aoyama K, Ishibashi K, Yuki R, Morinaga T, et al. Phosphorylation of KRAB-associated protein 1 (KAP1) at Tyr-449, Tyr-458, and Tyr-517 by nuclear tyrosine kinases inhibits the association of KAP1 and heterochromatin protein 1? (HP1?) with heterochromatin. J Biol Chem. 2013;288:17871-83 pubmed publisher
Soeda S, Nakayama Y, Honda T, Aoki A, Tamura N, Abe K, et al. v-Src causes delocalization of Mklp1, Aurora B, and INCENP from the spindle midzone during cytokinesis failure. Exp Cell Res. 2013;319:1382-97 pubmed publisher
Miekus K, Lukasiewicz E, Jarocha D, Sekula M, Drabik G, Majka M. The decreased metastatic potential of rhabdomyosarcoma cells obtained through MET receptor downregulation and the induction of differentiation. Cell Death Dis. 2013;4:e459 pubmed publisher
Hukkelhoven E, Liu Y, Yeh N, Ciznadija D, Blain S, Koff A. Tyrosine phosphorylation of the p21 cyclin-dependent kinase inhibitor facilitates the development of proneural glioma. J Biol Chem. 2012;287:38523-30 pubmed publisher
Wiedemann A, Rosselin M, Mijouin L, Bottreau E, Velge P. Involvement of c-Src tyrosine kinase upstream of class I phosphatidylinositol (PI) 3-kinases in Salmonella Enteritidis Rck protein-mediated invasion. J Biol Chem. 2012;287:31148-54 pubmed publisher
Nakayama Y, Matsui Y, Takeda Y, Okamoto M, Abe K, Fukumoto Y, et al. c-Src but not Fyn promotes proper spindle orientation in early prometaphase. J Biol Chem. 2012;287:24905-15 pubmed publisher
Fan P, McDaniel R, Kim H, Clagett D, Haddad B, Jordan V. Modulating therapeutic effects of the c-Src inhibitor via oestrogen receptor and human epidermal growth factor receptor 2 in breast cancer cell lines. Eur J Cancer. 2012;48:3488-98 pubmed publisher
Lau S, Shields D, Murphy E, Desgrosellier J, Anand S, Huang M, et al. EGFR-mediated carcinoma cell metastasis mediated by integrin ?v?5 depends on activation of c-Src and cleavage of MUC1. PLoS ONE. 2012;7:e36753 pubmed publisher
Willard M, Lajiness M, Wulur I, Feng B, Swearingen M, Uhlik M, et al. Somatic mutations in CCK2R alter receptor activity that promote oncogenic phenotypes. Mol Cancer Res. 2012;10:739-49 pubmed publisher
Zhong J, Baquiran J, Bonakdar N, Lees J, Ching Y, Pugacheva E, et al. NEDD9 stabilizes focal adhesions, increases binding to the extra-cellular matrix and differentially effects 2D versus 3D cell migration. PLoS ONE. 2012;7:e35058 pubmed publisher
Meiler E, Nieto Pelegrin E, Martinez Quiles N. Cortactin tyrosine phosphorylation promotes its deacetylation and inhibits cell spreading. PLoS ONE. 2012;7:e33662 pubmed publisher
Lahlou H, Sanguin Gendreau V, Frame M, Muller W. Focal adhesion kinase contributes to proliferative potential of ErbB2 mammary tumour cells but is dispensable for ErbB2 mammary tumour induction in vivo. Breast Cancer Res. 2012;14:R36 pubmed
Matsui Y, Nakayama Y, Okamoto M, Fukumoto Y, Yamaguchi N. Enrichment of cell populations in metaphase, anaphase, and telophase by synchronization using nocodazole and blebbistatin: a novel method suitable for examining dynamic changes in proteins during mitotic progression. Eur J Cell Biol. 2012;91:413-9 pubmed publisher
Rothschild S, Tschan M, Federzoni E, Jaggi R, Fey M, Gugger M, et al. MicroRNA-29b is involved in the Src-ID1 signaling pathway and is dysregulated in human lung adenocarcinoma. Oncogene. 2012;31:4221-32 pubmed publisher
Prinetti A, Cao T, Illuzzi G, Prioni S, Aureli M, Gagliano N, et al. A glycosphingolipid/caveolin-1 signaling complex inhibits motility of human ovarian carcinoma cells. J Biol Chem. 2011;286:40900-10 pubmed publisher
Guilluy C, Zhang Z, Bhende P, Sharek L, Wang L, Burridge K, et al. Latent KSHV infection increases the vascular permeability of human endothelial cells. Blood. 2011;118:5344-54 pubmed publisher
Mandal A, Shahidullah M, Beimgraben C, Delamere N. The effect of endothelin-1 on Src-family tyrosine kinases and Na,K-ATPase activity in porcine lens epithelium. J Cell Physiol. 2011;226:2555-61 pubmed publisher
Curtiss M, Hostager B, Stepniak E, Singh M, Manhica N, Knisz J, et al. Fyn binds to and phosphorylates T cell immunoglobulin and mucin domain-1 (Tim-1). Mol Immunol. 2011;48:1424-31 pubmed publisher
Baker A, Cox T, Bird D, Lang G, Murray G, Sun X, et al. The role of lysyl oxidase in SRC-dependent proliferation and metastasis of colorectal cancer. J Natl Cancer Inst. 2011;103:407-24 pubmed publisher
Srirangam A, Milani M, Mitra R, Guo Z, RODRIGUEZ M, Kathuria H, et al. The human immunodeficiency virus protease inhibitor ritonavir inhibits lung cancer cells, in part, by inhibition of survivin. J Thorac Oncol. 2011;6:661-70 pubmed publisher
Chao J, Gui P, Zamponi G, Davis G, Davis M. Spatial association of the Cav1.2 calcium channel with ?5?1-integrin. Am J Physiol Cell Physiol. 2011;300:C477-89 pubmed publisher
Destaing O, Planus E, Bouvard D, Oddou C, Badowski C, Bossy V, et al. ?1A integrin is a master regulator of invadosome organization and function. Mol Biol Cell. 2010;21:4108-19 pubmed publisher
Patwardhan P, Resh M. Myristoylation and membrane binding regulate c-Src stability and kinase activity. Mol Cell Biol. 2010;30:4094-107 pubmed publisher
Singh J, Aaronson S, Mlodzik M. Drosophila Abelson kinase mediates cell invasion and proliferation through two distinct MAPK pathways. Oncogene. 2010;29:4033-45 pubmed publisher
Schrage Y, Briaire de Bruijn I, de Miranda N, van Oosterwijk J, Taminiau A, van Wezel T, et al. Kinome profiling of chondrosarcoma reveals SRC-pathway activity and dasatinib as option for treatment. Cancer Res. 2009;69:6216-22 pubmed publisher
Cayer M, Proulx M, Ma X, Sakac D, Giguere J, Drouin M, et al. c-Src tyrosine kinase co-associates with and phosphorylates signal transducer and activator of transcription 5b which mediates the proliferation of normal human B lymphocytes. Clin Exp Immunol. 2009;156:419-27 pubmed publisher
Caron C, Spring K, Laramée M, Chabot C, Cloutier M, Gu H, et al. Non-redundant roles of the Gab1 and Gab2 scaffolding adapters in VEGF-mediated signalling, migration, and survival of endothelial cells. Cell Signal. 2009;21:943-53 pubmed publisher
Zimmerman B, Simaan M, Lee M, Luttrell L, Laporte S. c-Src-mediated phosphorylation of AP-2 reveals a general mechanism for receptors internalizing through the clathrin pathway. Cell Signal. 2009;21:103-10 pubmed publisher
Chabot C, Spring K, Gratton J, Elchebly M, Royal I. New role for the protein tyrosine phosphatase DEP-1 in Akt activation and endothelial cell survival. Mol Cell Biol. 2009;29:241-53 pubmed publisher
Kurata A, Katayama R, Watanabe T, Tsuruo T, Fujita N. TUSC4/NPRL2, a novel PDK1-interacting protein, inhibits PDK1 tyrosine phosphorylation and its downstream signaling. Cancer Sci. 2008;99:1827-34 pubmed publisher

product information

Catalog Number :

FCMAB253F

Subcategory :

Cell Signaling

Product Name :

Milli-Mark™Anti-Src-FITC Antibody, clone GD11

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P12931

Host Name :

Mouse

Antigen :

Milli-Mark™Src-FITC

Clone :

GD11

Conjugate :

FITC

Isotype :

IgG1κ

Product Description :

Milli-Mark™Anti-Src-FITC Antibody, clone GD11

Cross Reactivity :

Human

Background :

Src is a family of proto-oncogenic tyrosine kinases originally discovered by J. Michael Bishop and Harold E. Varmus. The discovery of Src family proteins has been instrumental to the modern understanding of cancer as a disease where normally healthy cellular signalling has gone awry. Some tumor-inducing retroviruses (such as Rous sarcoma virus (RSV)), contain a gene called v-src (viral-sarcoma). It was found that the v-src gene in RSV is required for the formation of cancer. Src tyrosine kinases transmit integrin-dependent signals central to cell movement and proliferation. Hallmarks of v-src induced transformation are rounding of the cell and the formation of actin rich podosomes on the basal surface of the cell. These structures are correlated with increased invasiveness, a process thought to be essential for metastasis. v-src lacks the C-terminal inhibitory phosphorylation site, and is therefore constitutively active as opposed to normal src (c-src) which is only activated under certain circumstances where it is required (e.g. growth factor signaling). v-src is therefore an instructive example of an oncogene whereas c-src is a proto-oncogene.

Immunogen :

Recombinant chicken pp60src purified from extracts of E. coli containing the plasmid plac-Src (Kuo, 2006)

Specificity :

Antibody recognizes Human Src.

Package Size :

100 tests

Uses :

Flow Cytometry

Storage :

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.