This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70
catalog :
FCMAB116P
quantity :
100 tests
clonality :
monoclonal
host :
mouse
conjugate :
PE
clone name :
MC-813-70
reactivity :
human
application :
immunocytochemistry, flow cytometry
citations: 28
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human
Anchan R, Lachke S, Gerami Naini B, Lindsey J, Ng N, Naber C, et al. Pax6- and Six3-mediated induction of lens cell fate in mouse and human ES cells. PLoS ONE. 2014;9:e115106 pubmed publisher
Zheng Y, Thomas A, Schmidt C, Dann C. Quantitative detection of human spermatogonia for optimization of spermatogonial stem cell culture. Hum Reprod. 2014;29:2497-511 pubmed publisher
Radan L, Hughes C, Teichroeb J, Vieira Zamora F, Jewer M, Postovit L, et al. Microenvironmental regulation of telomerase isoforms in human embryonic stem cells. Stem Cells Dev. 2014;23:2046-66 pubmed publisher
Tateno H, Onuma Y, Ito Y, Hiemori K, Aiki Y, Shimizu M, et al. A medium hyperglycosylated podocalyxin enables noninvasive and quantitative detection of tumorigenic human pluripotent stem cells. Sci Rep. 2014;4:4069 pubmed publisher
Miyazaki T, Nakatsuji N, Suemori H. Optimization of slow cooling cryopreservation for human pluripotent stem cells. Genesis. 2014;52:49-55 pubmed publisher
Lou Y, Kanninen L, Kuisma T, Niklander J, Noon L, Burks D, et al. The use of nanofibrillar cellulose hydrogel as a flexible three-dimensional model to culture human pluripotent stem cells. Stem Cells Dev. 2014;23:380-92 pubmed publisher
Stimpfel M, Skutella T, Cvjeticanin B, Meznaric M, Dovc P, Novakovic S, et al. Isolation, characterization and differentiation of cells expressing pluripotent/multipotent markers from adult human ovaries. Cell Tissue Res. 2013;354:593-607 pubmed publisher
Natunen S, Lampinen M, Suila H, Ritamo I, Pitkänen V, Nairn A, et al. Metabolic glycoengineering of mesenchymal stromal cells with N-propanoylmannosamine. Glycobiology. 2013;23:1004-12 pubmed publisher
Zeng L, Zhang P, Shi L, Yamamoto V, Lu W, Wang K. Functional impacts of NRXN1 knockdown on neurodevelopment in stem cell models. PLoS ONE. 2013;8:e59685 pubmed publisher
Verma R, Liu J, Holland M, Temple Smith P, Williamson M, Verma P. Nanog is an essential factor for induction of pluripotency in somatic cells from endangered felids. Biores Open Access. 2013;2:72-6 pubmed publisher
Onuma Y, Tateno H, Hirabayashi J, Ito Y, Asashima M. rBC2LCN, a new probe for live cell imaging of human pluripotent stem cells. Biochem Biophys Res Commun. 2013;431:524-9 pubmed publisher
Schrobback K, Wrobel J, Hutmacher D, Woodfield T, Klein T. Stage-specific embryonic antigen-4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells. Tissue Eng Part A. 2013;19:1316-26 pubmed publisher
Gottschling S, Jensen K, Warth A, Herth F, Thomas M, Schnabel P, et al. Stage-specific embryonic antigen-4 is expressed in basaloid lung cancer and associated with poor prognosis. Eur Respir J. 2013;41:656-63 pubmed publisher
Montserrat N, Ramirez Bajo M, Xia Y, Sancho Martinez I, Moya Rull D, Miquel Serra L, et al. Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2. J Biol Chem. 2012;287:24131-8 pubmed publisher
Wu D, Seita Y, Zhang X, Lu C, Roth M. Antibody-directed lentiviral gene transduction for live-cell monitoring and selection of human iPS and hES cells. PLoS ONE. 2012;7:e34778 pubmed publisher
Griscelli F, Feraud O, Oudrhiri N, Gobbo E, Casal I, Chomel J, et al. Malignant germ cell-like tumors, expressing Ki-1 antigen (CD30), are revealed during in vivo differentiation of partially reprogrammed human-induced pluripotent stem cells. Am J Pathol. 2012;180:2084-96 pubmed publisher
Jiang Y, Cowley S, Siler U, Melguizo D, Tilgner K, Browne C, et al. Derivation and functional analysis of patient-specific induced pluripotent stem cells as an in vitro model of chronic granulomatous disease. Stem Cells. 2012;30:599-611 pubmed publisher
Verma R, Holland M, Temple Smith P, Verma P. Inducing pluripotency in somatic cells from the snow leopard (Panthera uncia), an endangered felid. Theriogenology. 2012;77:220-8, 228.e1-2 pubmed publisher
Silk K, Silk J, Ichiryu N, Davies T, Nolan K, Leishman A, et al. Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells. Gene Ther. 2012;19:1035-40 pubmed publisher
Vaculik C, Schuster C, Bauer W, Iram N, Pfisterer K, Kramer G, et al. Human dermis harbors distinct mesenchymal stromal cell subsets. J Invest Dermatol. 2012;132:563-74 pubmed publisher
Sánchez Danés A, Consiglio A, Richaud Y, Rodriguez Piza I, Dehay B, Edel M, et al. Efficient generation of A9 midbrain dopaminergic neurons by lentiviral delivery of LMX1A in human embryonic stem cells and induced pluripotent stem cells. Hum Gene Ther. 2012;23:56-69 pubmed publisher
Fu X, Toh W, Liu H, Lu K, Li M, Cao T. Establishment of clinically compliant human embryonic stem cells in an autologous feeder-free system. Tissue Eng Part C Methods. 2011;17:927-37 pubmed publisher
Suila H, Pitkänen V, Hirvonen T, Heiskanen A, Anderson H, Laitinen A, et al. Are globoseries glycosphingolipids SSEA-3 and -4 markers for stem cells derived from human umbilical cord blood?. J Mol Cell Biol. 2011;3:99-107 pubmed publisher
Karbanova J, Soukup T, Suchanek J, Pytlik R, Corbeil D, Mokry J. Characterization of dental pulp stem cells from impacted third molars cultured in low serum-containing medium. Cells Tissues Organs. 2011;193:344-65 pubmed publisher
Li T, Mai Q, Gao J, Zhou C. Cryopreservation of human embryonic stem cells with a new bulk vitrification method. Biol Reprod. 2010;82:848-53 pubmed publisher
Rowland T, Miller L, Blaschke A, Doss E, Bonham A, Hikita S, et al. Roles of integrins in human induced pluripotent stem cell growth on Matrigel and vitronectin. Stem Cells Dev. 2010;19:1231-40 pubmed publisher
Battula V, Treml S, Bareiss P, Gieseke F, Roelofs H, de Zwart P, et al. Isolation of functionally distinct mesenchymal stem cell subsets using antibodies against CD56, CD271, and mesenchymal stem cell antigen-1. Haematologica. 2009;94:173-84 pubmed publisher
Heiskanen A, Hirvonen T, Salo H, Impola U, Olonen A, Laitinen A, et al. Glycomics of bone marrow-derived mesenchymal stem cells can be used to evaluate their cellular differentiation stage. Glycoconj J. 2009;26:367-84 pubmed publisher
product information
Catalog Number :
FCMAB116P
Subcategory :
Stem Cell Research
Product Name :
Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Host Name :
Mouse
Antigen :
SSEA-4-PE
Clone :
MC-813-70
Conjugate :
Phycoerythrin
Isotype :
IgG3κ
Product Description :
Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70
Cross Reactivity :
Human
Background :
The cell-surface markers used routinely to identify undifferentiated human embryonic stem cells (hESCs) were initially characterized as markers for mESCs, mouse embryonic carcinomas (ECs), or human EC cells. Stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 are widely used as cell-surface markers to define both human and mouse ESCs. SSEA-3 and SSEA-4 are expressed on undifferentiated hESCs but not on undifferentiated mESCs.
Immunogen :
Human embryonal carcinoma cell line 2102Ep.
Specificity :
This antibody reacts with the stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In ctontrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.
Package Size :
100 tests
Uses :
Flow Cytometry;Immunocytochemistry
Storage :
Maintain refrigerated at 2-8oC protected from light in undiluted aliquots for up to 6 months from date of receipt.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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