Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor® 488 conjugated | EMD Millipore FCMAB101A4 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor® 488 conjugated

catalog :

FCMAB101A4

quantity :

100 tests

clonality :

monoclonal

host :

mouse

conjugate :

AF488

clone name :

BU-1

application :

immunocytochemistry, flow cytometry

citations: 5

Reference
Dos Santos E, Carneiro Lobo T, Aoki M, Levantini E, BassÃ¨res D. Aurora kinase targeting in lung cancer reduces KRAS-induced transformation. Mol Cancer. 2016;15:12 pubmed publisher
Kang S, Keasey M, Hagg T. P2X7 receptor inhibition increases CNTF in the subventricular zone, but not neurogenesis or neuroprotection after stroke in adult mice. Transl Stroke Res. 2013;4:533-45 pubmed publisher
Kang S, Keasey M, Arnold S, Reid R, Geralds J, Hagg T. Endogenous CNTF mediates stroke-induced adult CNS neurogenesis in mice. Neurobiol Dis. 2013;49:68-78 pubmed publisher
Arnold S, Hagg T. Serotonin 1A receptor agonist increases species- and region-selective adult CNS proliferation, but not through CNTF. Neuropharmacology. 2012;63:1238-47 pubmed publisher
Mahoney E, Benton R, Maddie M, Whittemore S, Hagg T. ADAM8 is selectively up-regulated in endothelial cells and is associated with angiogenesis after spinal cord injury in adult mice. J Comp Neurol. 2009;512:243-55 pubmed publisher

product information

Catalog Number :

FCMAB101A4

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor® 488 conjugated

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

BrdU

Clone :

Bu-1

Conjugate :

AlexaFluor®488

Isotype :

IgG2a

Product Description :

Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor® 488 conjugated

Cross Reactivity :

Human

Background :

Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. This antibody does not require pretreatment of cells with strong DNA denaturants in order for the antibody to recognize incorporated BrdU. The antibody's special ability is thought to be due to the presence of DNAse in the antibody solution arising from the mycoplasma infecting the original hybridoma line {Gonchoroff, 1985}.

ALT Names :

BrdU

Immunogen :

Bromodeoxyuridine

Specificity :

Specifically binds to bromodeoxyuridine (crossreacts with iodouridine 10%). Does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine.

Package Size :

100 tests

Uses :

Flow Cytometry;Immunocytochemistry

Storage :

Stable for 6 months at 2-8°C from date of receipt. Protect from light.