PCNA Antibody, clone PC10 | EMD Millipore CBL407 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PCNA Antibody, clone PC10

catalog :

CBL407

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PC10

The same clone is also sold as:

Exbio: 1P-182-T100, 11-182-C100, 1P-182-T025
Invitrogen: 13-3940, 13-3900, MA5-11358
OriGene: TA309795, AM10035PU-N, AM10035PU-S
Novus Biologicals: NBP2-80905, NB500-106
Bio-Rad: MCA1558, MCA1558P, MCA1558F
LifeSpan Biosciences: LS-C86889, LS-C87459, LS-C9535, LS-C170844, LS-B3434, LS-C43736, LS-C358014, LS-C87502, LS-C87503, LS-B9987
GeneTex: GTX20029
BioLegend: 307909, 307912, 307904, 307902, 307901, 307908, 307914
Abcam: ab29
Santa Cruz Biotechnology: sc-56 HRP, sc-56 AC, sc-56
Enzo Life Sciences: ALX-801-091-L001
Cell Signaling Technology: 2586, 8580

reactivity :

human, mouse, rat, zebrafish

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

citations: 34

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:300; loading ...; fig 2a	Guven A, Kalebic N, Long K, Florio M, Vaid S, Brandl H, et al. Extracellular matrix-inducing Sox9 promotes both basal progenitor proliferation and gliogenesis in developing neocortex. elife. 2020;9: pubmed publisher
western blot; human; fig 6a	Wilson R, Biasutto A, Wang L, Fischer R, Baple E, Crosby A, et al. PCNA dependent cellular activities tolerate dramatic perturbations in PCNA client interactions. DNA Repair (Amst). 2017;50:22-35 pubmed publisher
immunohistochemistry - paraffin section; zebrafish ; 1:100; fig 5	He S, Mansour M, Zimmerman M, Ki D, Layden H, Akahane K, et al. Synergy between loss of NF1 and overexpression of MYCN in neuroblastoma is mediated by the GAP-related domain. elife. 2016;5: pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:100; fig 3	Kretzschmar K, Cottle D, Donati G, Chiang M, Quist S, Gollnick H, et al. BLIMP1 is required for postnatal epidermal homeostasis but does not define a sebaceous gland progenitor under steady-state conditions. Stem Cell Reports. 2014;3:620-33 pubmed publisher
western blot; mouse	Adeyemi R, Fuller M, Pintel D. Efficient parvovirus replication requires CRL4Cdt2-targeted depletion of p21 to prevent its inhibitory interaction with PCNA. PLoS Pathog. 2014;10:e1004055 pubmed publisher
immunohistochemistry - paraffin section; mouse	Hughes M, Jiang T, Lin S, Leung Y, Kobielak K, Widelitz R, et al. Disrupted ectodermal organ morphogenesis in mice with a conditional histone deacetylase 1, 2 deletion in the epidermis. J Invest Dermatol. 2014;134:24-32 pubmed publisher
western blot; mouse; 1:1000	Chen C, Sloane J, Li H, Aytan N, Giannaris E, Zeldich E, et al. The antiaging protein Klotho enhances oligodendrocyte maturation and myelination of the CNS. J Neurosci. 2013;33:1927-39 pubmed publisher
	Kuroda Y, Hayashi S, Hagio S, Abe M, Furukawa S, Nakae D. Pancreatic Ductal Adenocarcinoma in a Wistar Hannover GALAS Rat. J Toxicol Pathol. 2014;27:147-51 pubmed publisher
	Cazzalini O, Sommatis S, Tillhon M, Dutto I, Bachi A, Rapp A, et al. CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis. Nucleic Acids Res. 2014;42:8433-48 pubmed publisher
	Gill R, Michael A, Westley L, Kocher H, Murphy J, Dhoot G. SULF1/SULF2 splice variants differentially regulate pancreatic tumour growth progression. Exp Cell Res. 2014;324:157-71 pubmed publisher
	Chen X, Paudyal S, Chin R, You Z. PCNA promotes processive DNA end resection by Exo1. Nucleic Acids Res. 2013;41:9325-38 pubmed publisher
	Edelmann K, Glashauser L, Sprungala S, Hesl B, Fritschle M, Ninkovic J, et al. Increased radial glia quiescence, decreased reactivation upon injury and unaltered neuroblast behavior underlie decreased neurogenesis in the aging zebrafish telencephalon. J Comp Neurol. 2013;521:3099-115 pubmed publisher
	Karger C, Kurtz F, Steppan D, Schwarzensteiner I, Machura K, Angel P, et al. Procollagen I-expressing renin cell precursors. Am J Physiol Renal Physiol. 2013;305:F355-61 pubmed publisher
	Niwa N, Akimoto Kato A, Sakuma M, Kuraku S, Hayashi S. Homeogenetic inductive mechanism of segmentation in polychaete tail regeneration. Dev Biol. 2013;381:460-70 pubmed publisher
	Akane H, Shiraki A, Imatanaka N, Akahori Y, Itahashi M, Ohishi T, et al. Glycidol induces axonopathy by adult-stage exposure and aberration of hippocampal neurogenesis affecting late-stage differentiation by developmental exposure in rats. Toxicol Sci. 2013;134:140-54 pubmed publisher
	Solarczyk K, Zarebski M, Dobrucki J. Inducing local DNA damage by visible light to study chromatin repair. DNA Repair (Amst). 2012;11:996-1002 pubmed publisher
	Tillhon M, Cazzalini O, Nardo T, Necchi D, Sommatis S, Stivala L, et al. p300/CBP acetyl transferases interact with and acetylate the nucleotide excision repair factor XPG. DNA Repair (Amst). 2012;11:844-52 pubmed publisher
	Beck H, Nähse Kumpf V, Larsen M, O Hanlon K, Patzke S, Holmberg C, et al. Cyclin-dependent kinase suppression by WEE1 kinase protects the genome through control of replication initiation and nucleotide consumption. Mol Cell Biol. 2012;32:4226-36 pubmed
	Ng C, Wu P, Foley J, Foley A, McDonald M, Juan W, et al. The chicken frizzle feather is due to an ?-keratin (KRT75) mutation that causes a defective rachis. PLoS Genet. 2012;8:e1002748 pubmed publisher
	Lee K, Rhee K. Separase-dependent cleavage of pericentrin B is necessary and sufficient for centriole disengagement during mitosis. Cell Cycle. 2012;11:2476-85 pubmed publisher
	Tichy E, Pillai R, Deng L, Tischfield J, Hexley P, Babcock G, et al. The abundance of Rad51 protein in mouse embryonic stem cells is regulated at multiple levels. Stem Cell Res. 2012;9:124-34 pubmed publisher
	Islam M, Fujinaga R, Yanai A, Jahan M, Takeshita Y, Kokubu K, et al. Characterization of the "sporadically lurking HAP1-immunoreactive (SLH) cells" in the hippocampus, with special reference to the expression of steroid receptors, GABA, and progenitor cell markers. Neuroscience. 2012;210:67-81 pubmed publisher
	Postnikov Y, Kurahashi T, Zhou M, Bustin M. The nucleosome binding protein HMGN1 interacts with PCNA and facilitates its binding to chromatin. Mol Cell Biol. 2012;32:1844-54 pubmed publisher
	Muscolini M, Montagni E, Palermo V, Di Agostino S, Gu W, Abdelmoula Souissi S, et al. The cancer-associated K351N mutation affects the ubiquitination and the translocation to mitochondria of p53 protein. J Biol Chem. 2011;286:39693-702 pubmed publisher
	Yao R, Natsume Y, Saiki Y, Shioya H, Takeuchi K, Yamori T, et al. Disruption of Tacc3 function leads to in vivo tumor regression. Oncogene. 2012;31:135-48 pubmed publisher
	Yang G, Hinson M, Bordner J, Lin Q, Fernando A, La P, et al. Silencing hyperoxia-induced C/EBP? in neonatal mice improves lung architecture via enhanced proliferation of alveolar epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2011;301:L187-96 pubmed publisher
	Klemm D, Majka S, Crossno J, Psilas J, Reusch J, Garat C. Reduction of reactive oxygen species prevents hypoxia-induced CREB depletion in pulmonary artery smooth muscle cells. J Cardiovasc Pharmacol. 2011;58:181-91 pubmed publisher
	Pritchard M, Malinak R, Nagy L. Early growth response (EGR)-1 is required for timely cell-cycle entry and progression in hepatocytes after acute carbon tetrachloride exposure in mice. Am J Physiol Gastrointest Liver Physiol. 2011;300:G1124-31 pubmed publisher
	Maurer C, Schonthaler H, Mueller K, Neuhauss S. Distinct retinal deficits in a zebrafish pyruvate dehydrogenase-deficient mutant. J Neurosci. 2010;30:11962-72 pubmed publisher
	Beck H, Nähse V, Larsen M, Groth P, Clancy T, Lees M, et al. Regulators of cyclin-dependent kinases are crucial for maintaining genome integrity in S phase. J Cell Biol. 2010;188:629-38 pubmed publisher
	Munro S, Khaire N, Inche A, Carr S, La Thangue N. Lysine methylation regulates the pRb tumour suppressor protein. Oncogene. 2010;29:2357-67 pubmed publisher
	Reimer M, Sörensen I, Kuscha V, Frank R, Liu C, Becker C, et al. Motor neuron regeneration in adult zebrafish. J Neurosci. 2008;28:8510-6 pubmed publisher
	Brun J, Chiu R, Lockhart K, Xiao W, Wouters B, Gray D. hMMS2 serves a redundant role in human PCNA polyubiquitination. BMC Mol Biol. 2008;9:24 pubmed publisher
	von Lukowicz T, Hassa P, Lohmann C, Boren J, Braunersreuther V, Mach F, et al. PARP1 is required for adhesion molecule expression in atherogenesis. Cardiovasc Res. 2008;78:158-66 pubmed

product information

Catalog Number :

CBL407

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-PCNA Antibody, clone PC10

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P12004

Host Name :

Mouse

Antigen :

PCNA

Clone :

PC10

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-PCNA Antibody, clone PC10

Cross Reactivity :

Invertebrates;Vertebrates

Background :

Proliferating cell nuclear antigen (PCNA) is a 36 kDa molecule that is highly conserved between species. PCNA was first identified as the antigen for a subpopulation of autoantibodies in patients with systemic lupus erythematosus (Miyachi, 1978; Takasaki, 1984; Ogata, 1987). It has since been determined that PCNA serves as a co-factor for DNA polymerase delta in S-phase as well as during DNA synthesis associated with mechanisms involved in DNA damage repair (Tan, 1987; Bravo, 1987). The temporal specificity of PCNA expression makes it an ideal marker for cell proliferation. PCNA begins to accumulate during the G1 phase of the cell cycle, is most abundant during the S phase, and declines during the G2/M phase (Kurki, 1988). Since the half-life of PCNA exceeds 20 hours, it may be possible to detect the protein in non-cycling cells.

ALT Names :

Proliferating Cell Nuclear Antigen; DNA Polymerase delta Processivity Factor

Immunogen :

Rat PCNA made in the protein A vector pR1T2T.

Specificity :

Clone PC10 recognizes PCNA from all vertebrate and insect species tested and has been used to identify transformed cells (Kurki, 1988), proliferating cells in solid tumors (Smetana, 1983), and blast cells in leukemia patients (Takasaki, 1984). Immunohistochemistry with clone PC10 has been used to study the expression of PCNA in paraffin sections of normal tissues and lymphoid neoplasms (Hall, 1990). In proliferating cells, PCNA staining pattern is predominantly nuclear. By western blot, the antibody detects a polypeptide migrating at 36 kDa with an isoelectric point of 4.8.

Package Size :

100 µg

Uses :

ELISA;Flow Cytometry;Immunoprecipitation;Western Blotting;Immunohistochemistry (Paraffin)

Storage :

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C. Avoid repeated freeze/ thawing.