p53 Antibody, aa 211-220, clone240 | EMD Millipore CBL404 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

p53 Antibody, aa 211-220, clone240

catalog :

CBL404

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PAb240

The same clone is also sold as:

Invitrogen: 13-4100
GeneTex: GTX70218
LifeSpan Biosciences: LS-C134560, LS-C134559, LS-C134561
ImmuQuest: IQ624FITC, IQ624HRP
Abnova: MAB6963

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunoprecipitation

citations: 18

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:5000; loading ...; fig 10a	Kalita B, Ranjan R, Singh A, Yashavarddhan M, Bajaj S, Gupta M. A Combination of Podophyllotoxin and Rutin Attenuates Radiation Induced Gastrointestinal Injury by Negatively Regulating NF-?B/p53 Signaling in Lethally Irradiated Mice. PLoS ONE. 2016;11:e0168525 pubmed publisher
western blot; mouse; 1:700; loading ...; fig 4a	Yang J, Platt L, Maity B, Ahlers K, Luo Z, Lin Z, et al. RGS6 is an essential tumor suppressor that prevents bladder carcinogenesis by promoting p53 activation and DNMT1 downregulation. Oncotarget. 2016;7:69159-69172 pubmed publisher
western blot; human; loading ...; fig s1 i	Wang X, Li S, Wang G, Ma Z, Chuai M, Cao L, et al. High glucose environment inhibits cranial neural crest survival by activating excessive autophagy in the chick embryo. Sci Rep. 2015;5:18321 pubmed publisher
immunoprecipitation; mouse; fig 4 immunoprecipitation; human; fig 6	Huang Y, Bulavin D. Oncogene-mediated regulation of p53 ISGylation and functions. Oncotarget. 2014;5:5808-18 pubmed
	Liu W, Yang L, Liu Y, Yuan D, Zhao Z, Wang Q, et al. Dynamic characterization of intestinal metaplasia in the gastric corpus mucosa of Atp4a-deficient mice. Biosci Rep. 2020;40: pubmed publisher
	Liu W, Zhao Z, Liu Y, Pan H, Lin L. Weipiling ameliorates gastric precancerous lesions in Atp4a-/- mice. BMC Complement Altern Med. 2019;19:318 pubmed publisher
	Aggarwal M, Saxena R, Asif N, Sinclair E, Tan J, Cruz I, et al. p53 mutant-type in human prostate cancer cells determines the sensitivity to phenethyl isothiocyanate induced growth inhibition. J Exp Clin Cancer Res. 2019;38:307 pubmed publisher
	KavaliauskaitÄ— D, StakiÅ¡aitis D, MartinkutÄ— J, Å lekienÄ— L, Kazlauskas A, BalnytÄ— I, et al. The Effect of Sodium Valproate on the Glioblastoma U87 Cell Line Tumor Development on the Chicken Embryo Chorioallantoic Membrane and on EZH2 and p53 Expression. Biomed Res Int. 2017;2017:6326053 pubmed publisher
	Aggarwal M, Saxena R, Sinclair E, Fu Y, Jacobs A, Dyba M, et al. Reactivation of mutant p53 by a dietary-related compound phenethyl isothiocyanate inhibits tumor growth. Cell Death Differ. 2016;23:1615-27 pubmed publisher
	Vassilopoulos A, Tominaga Y, Kim H, Lahusen T, Li B, Yu H, et al. WEE1 murine deficiency induces hyper-activation of APC/C and results in genomic instability and carcinogenesis. Oncogene. 2015;34:3023-35 pubmed publisher
	Saha M, Jiang H, Yang Y, Reece D, Chang H. PRIMA-1Met/APR-246 displays high antitumor activity in multiple myeloma by induction of p73 and Noxa. Mol Cancer Ther. 2013;12:2331-41 pubmed publisher
	Takahashi R, Takeshita F, Honma K, Ono M, Kato K, Ochiya T. Ribophorin II regulates breast tumor initiation and metastasis through the functional suppression of GSK3?. Sci Rep. 2013;3:2474 pubmed publisher
	Lanni C, Necchi D, Pinto A, Buoso E, Buizza L, Memo M, et al. Zyxin is a novel target for ?-amyloid peptide: characterization of its role in Alzheimer's pathogenesis. J Neurochem. 2013;125:790-9 pubmed publisher
	Koch A, Hatina J, Rieder H, Seifert H, Huckenbeck W, Jankowiak F, et al. Discovery of TP53 splice variants in two novel papillary urothelial cancer cell lines. Cell Oncol (Dordr). 2012;35:243-57 pubmed publisher
	Di Conza G, Mancini F, Buttarelli M, Pontecorvi A, Trimarchi F, Moretti F. MDM4 enhances p53 stability by promoting an active conformation of the protein upon DNA damage. Cell Cycle. 2012;11:749-60 pubmed publisher
	Puca R, Nardinocchi L, Porru M, Simon A, Rechavi G, Leonetti C, et al. Restoring p53 active conformation by zinc increases the response of mutant p53 tumor cells to anticancer drugs. Cell Cycle. 2011;10:1679-89 pubmed
	Blickwedehl J, Agarwal M, Seong C, Pandita R, Melendy T, Sung P, et al. Role for proteasome activator PA200 and postglutamyl proteasome activity in genomic stability. Proc Natl Acad Sci U S A. 2008;105:16165-70 pubmed publisher
	Yamamoto H, Ozaki T, Nakanishi M, Kikuchi H, Yoshida K, Horie H, et al. Oxidative stress induces p53-dependent apoptosis in hepatoblastoma cell through its nuclear translocation. Genes Cells. 2007;12:461-71 pubmed

product information

Catalog Number :

CBL404

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-p53 Antibody, aa 211-220, clone240

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P04637

Host Name :

Mouse

Antigen :

p53

Clone :

PAb240

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-p53 Antibody, aa 211-220, clone240

Cross Reactivity :

Bovine;Chicken;Human;Hamster;Mouse;Monkey;Rat

Background :

p53 was discovered in 1979 as a cellular protein associating with the transforming protein of SV40 tumor virus. Since then, many different biochemical functions have been attributed to the 53 kD phosphoprotein. Experimental evidence has suggested that p53 acts as a negative regulator of cell growth in normal cells (Finlay, 1989). Thus, the inactivation or mutation of p53 may be an essential step in the development of malignancy (Lane and Benchmol, 1990). Wild-type p53 levels in normal cells and tissues were found to be very low. Mutant p53 polypeptide, however, is often found to be present at high concentrations in mammalian tumors and tumor cell lines. For example, in an immuno-histochemistry study 40% of human breast cancer showed elevated levels of mutant p53 in the cell nucleus. Mutations of the p53 protein have some characteristic features: a) Most of them are missense point mutations giving rise to an altered protein function. b) Many -but not all- mutant p53 proteins exhibit a common mutant structure, which can be recognized by monoclonal antibodies specific for p53 in the mutant conformation.

Immunogen :

Murine p53-beta galactosidase fusion protein expressed in E. coli.

Specificity :

PAb 240 recognizes an epitope of p53 tumor suppressor protein between amino acids 211 and 220 (Gannon, 1990; Legros, 1994) in human, mouse, rat, hamster, monkey, cow and chicken. Assaying native samples (immunoprecipitation, ELISA) the PAb 240 detects only the mutant forms of p53 (Gannon, 1990; Said, 1994). In methods using denatured samples [Western blot analysis (Gannon, 1990) and immunohistochemistry of frozen tissue sections (Said, 1994; Bartek, 1991; Walker, 1991)], the PAb 240 recognizes both mutant and denatured wild-type p53.

Package Size :

100 µg

Uses :

Immunoprecipitation;Western Blotting;Immunohistochemistry

Storage :

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.