Vimentin Antibody, clone VIM 3B4 | EMD Millipore CBL202 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Vimentin Antibody, clone VIM 3B4

catalog :

CBL202

quantity :

50 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

VIM 3B4

The same clone is also sold as:

Dako: M7020
LifeSpan Biosciences: LS-C85521, LS-C193364, LS-C86895
Leica Biosystems: NCL-VIM-V9
OriGene: BM5501, BM5501F

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

citations: 32

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; loading ...; fig st15 immunohistochemistry - paraffin section; rat; loading ...; fig st15	Furukawa S, Nagaike M, Ozaki K. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol. 2017;30:79-107 pubmed publisher
western blot; human	Lee D, Su J, Kim H, Chang B, Papatsenko D, Zhao R, et al. Modeling familial cancer with induced pluripotent stem cells. Cell. 2015;161:240-54 pubmed publisher
immunohistochemistry - paraffin section; human; 1:1,000	Kim W, Barron D, San Martin R, Chan K, Tran L, Yang F, et al. RUNX1 is essential for mesenchymal stem cell proliferation and myofibroblast differentiation. Proc Natl Acad Sci U S A. 2014;111:16389-94 pubmed publisher
western blot; human; 1:1000	Brueggmann D, Templeman C, Starzinski Powitz A, Rao N, Gayther S, Lawrenson K. Novel three-dimensional in vitro models of ovarian endometriosis. J Ovarian Res. 2014;7:17 pubmed publisher
immunocytochemistry; human	Liu X, Dong D, Sun P, Du J, Gu L, Ge Y. Expression and location of alpha-fetoprotein during rat colon development. World J Gastroenterol. 2009;15:1738-43 pubmed
	Gurrapu S, Franzolin G, Fard D, Accardo M, Medico E, Sarotto I, et al. Reverse signaling by semaphorin 4C elicits SMAD1/5- and ID1/3-dependent invasive reprogramming in cancer cells. Sci Signal. 2019;12: pubmed publisher
	Bailey R, Armao D, Nagabhushan Kalburgi S, Gray S. Development of Intrathecal AAV9 Gene Therapy for Giant Axonal Neuropathy. Mol Ther Methods Clin Dev. 2018;9:160-171 pubmed publisher
	Kuo W, Hwu L, Wu C, Lee J, Chang C, Liu R. STAT3/NF-?B-Regulated Lentiviral TK/GCV Suicide Gene Therapy for Cisplatin-Resistant Triple-Negative Breast Cancer. Theranostics. 2017;7:647-663 pubmed publisher
	Wu Y, Cheng M, Shi Z, Feng Z, Guan X. Dynamic expression and localization of c-MET isoforms in the developing rat pancreas. Int J Clin Exp Pathol. 2014;7:8563-72 pubmed
	Zhang B, Yang Y, Shi X, Liao W, Chen M, Cheng A, et al. Proton pump inhibitor pantoprazole abrogates adriamycin-resistant gastric cancer cell invasiveness via suppression of Akt/GSK-Î²/Î²-catenin signaling and epithelial-mesenchymal transition. Cancer Lett. 2015;356:704-12 pubmed publisher
	Kuroda Y, Hayashi S, Hagio S, Abe M, Furukawa S, Nakae D. Pancreatic Ductal Adenocarcinoma in a Wistar Hannover GALAS Rat. J Toxicol Pathol. 2014;27:147-51 pubmed publisher
	Ampollini L, Madeddu D, Falco A, Frati C, Lorusso B, Graiani G, et al. Lung mesenchymal cells function as an inductive microenvironment for human lung cancer propagating cellsâ€ . Eur J Cardiothorac Surg. 2014;46:e103-12 pubmed publisher
	Lawrenson K, Lee N, Torres H, Lee J, Brueggmann D, Rao P, et al. Src as a novel therapeutic target for endometriosis. Gynecol Oncol. 2014;135:100-7 pubmed publisher
	Pereyra Bonnet F, Gimeno M, Argumedo N, Ielpi M, Cardozo J, GimÃ©nez C, et al. Skin fibroblasts from patients with type 1 diabetes (T1D) can be chemically transdifferentiated into insulin-expressing clusters: a transgene-free approach. PLoS ONE. 2014;9:e100369 pubmed publisher
	Zayas Santiago A, Agte S, Rivera Y, Benedikt J, Ulbricht E, Karl A, et al. Unidirectional photoreceptor-to-Müller glia coupling and unique K+ channel expression in Caiman retina. PLoS ONE. 2014;9:e97155 pubmed publisher
	Zhu H, Zhang H, Jin F, Fang M, Huang M, Yang C, et al. Elevated Orai1 expression mediates tumor-promoting intracellular Ca2+ oscillations in human esophageal squamous cell carcinoma. Oncotarget. 2014;5:3455-71 pubmed
	Yip Y, Pang P, Deng W, Tsang C, Zeng M, Hau P, et al. Efficient immortalization of primary nasopharyngeal epithelial cells for EBV infection study. PLoS ONE. 2013;8:e78395 pubmed publisher
	Lazzari M, Bettini S, Franceschini V. Immunocytochemical characterisation of olfactory ensheathing cells of zebrafish. J Anat. 2014;224:192-206 pubmed publisher
	Jeong W, Lim W, Ahn S, Lim C, Lee J, Bae S, et al. Recrudescence mechanisms and gene expression profile of the reproductive tracts from chickens during the molting period. PLoS ONE. 2013;8:e76784 pubmed publisher
	Lee J, Mhawech Fauceglia P, Lee N, Parsanian L, Lin Y, Gayther S, et al. A three-dimensional microenvironment alters protein expression and chemosensitivity of epithelial ovarian cancer cells in vitro. Lab Invest. 2013;93:528-42 pubmed publisher
	Mussche S, Devreese B, Nagabhushan Kalburgi S, Bachaboina L, Fox J, Shih H, et al. Restoration of cytoskeleton homeostasis after gigaxonin gene transfer for giant axonal neuropathy. Hum Gene Ther. 2013;24:209-19 pubmed publisher
	Barczyk M, Lu N, Popova S, Bolstad A, Gullberg D. ?11?1 integrin-mediated MMP-13-dependent collagen lattice contraction by fibroblasts: evidence for integrin-coordinated collagen proteolysis. J Cell Physiol. 2013;228:1108-19 pubmed publisher
	Lazzari M, Bettini S, Franceschini V. Immunocytochemical characterization of olfactory ensheathing cells in fish. Brain Struct Funct. 2013;218:539-49 pubmed publisher
	Davalos V, Moutinho C, Villanueva A, Boque R, Silva P, Carneiro F, et al. Dynamic epigenetic regulation of the microRNA-200 family mediates epithelial and mesenchymal transitions in human tumorigenesis. Oncogene. 2012;31:2062-74 pubmed publisher
	Xi C, Chen Q, Zeng S, Lin Y, Huang Y, Liu Y, et al. Sites of origin and developmental dynamics of the neurons in the core and shell regions of torus semicircularis in the Chinese softshell turtle (Pelodiscus sinensis). J Comp Neurol. 2011;519:2677-96 pubmed publisher
	Tauber S, Jais A, Jeitler M, Haider S, Husa J, Lindroos J, et al. Transcriptome analysis of human cancer reveals a functional role of heme oxygenase-1 in tumor cell adhesion. Mol Cancer. 2010;9:200 pubmed publisher
	Hryciw T, MacDonald J, Phillips R, Seah C, Pasternak S, Meakin S. The fibroblast growth factor receptor substrate 3 adapter is a developmentally regulated microtubule-associated protein expressed in migrating and differentiated neurons. J Neurochem. 2010;112:924-39 pubmed publisher
	Wang D, Zhao Z, Caperell Grant A, Yang G, Mok S, Liu J, et al. S1P differentially regulates migration of human ovarian cancer and human ovarian surface epithelial cells. Mol Cancer Ther. 2008;7:1993-2002 pubmed publisher
	Hou L, Wang Y, Liu L, Guo J, Teng L, Cao L, et al. Expression and localization of mesothelin in developing rat pancreas. Dev Growth Differ. 2008;50:531-41 pubmed publisher
	Perez Alvarez M, Isiegas C, Santano C, Salazar J, Ramirez A, Trivino A, et al. Vimentin isoform expression in the human retina characterized with the monoclonal antibody 3CB2. J Neurosci Res. 2008;86:1871-83 pubmed publisher
	Liu L, Guo J, Yuan L, Cheng M, Cao L, Shi H, et al. Alpha-fetoprotein is dynamically expressed in rat pancreas during development. Dev Growth Differ. 2007;49:669-81 pubmed
	Yang P, Baker K, Hagg T. A disintegrin and metalloprotease 21 (ADAM21) is associated with neurogenesis and axonal growth in developing and adult rodent CNS. J Comp Neurol. 2005;490:163-79 pubmed

product information

Catalog Number :

CBL202

Subcategory :

Cell Structure

Product Name :

Anti-Vimentin Antibody, clone VIM 3B4

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P08670

Host Name :

Mouse

Antigen :

Vimentin

Clone :

VIM 3B4

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-Vimentin Antibody, clone VIM 3B4

Cross Reactivity :

Amphibian;Bovine;Canine;Chicken;Human;Monkey

Immunogen :

Vimentin purified from bovine lens

Specificity :

Highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This antibody is positive with cells of mesenchymal derivation, including endothelial cells and certain smooth muscle cells of the vascular tract, fibroblasts, connective tissue, all types of blood cells, including thymocytes, interstitial and testicular Sertoli cells, ovarian follicle cells. It can also be used to detect coexpression of vimentin in combination with other intermediate filament proteins. VIM 3B4 has turned out to be the most avid monoclonal antibody to vimentin. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al. (1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995). Polypeptide reacting: MW 57 kDa intermediate filament protein (vimentin) of mesenchymal cells.

Package Size :

50 μg

Uses :

ELISA;Immunofluorescence;Immunohistochemistry (Paraffin);Western Blotting

Storage :

Maintain at 2–8°C for up to 12 months from date of receipt.