This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Actin Antibody, smooth muscle, clone ASM-1
catalog :
CBL171
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
ASM-1

The same clone is also sold as:
reactivity :
African green monkey, human, mouse, rat, chicken, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section
citations: 43
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; human; 1:50; loading ...; fig 3b
Wu M, Liu S, Gao Y, Bai H, Machairaki V, Li G, et al. Conditional gene knockout and reconstitution in human iPSCs with an inducible Cas9 system. Stem Cell Res. 2018;29:6-14 pubmed publisher
  • immunocytochemistry; human; 1:50; loading ...; tbl 1
Liu S, Ye Z, Gao Y, He C, Williams D, MOLITERNO A, et al. Generation of human iPSCs from an essential thrombocythemia patient carrying a V501L mutation in the MPL gene. Stem Cell Res. 2017;18:57-59 pubmed publisher
  • immunocytochemistry; human; fig s1e
Yang Y, Liu B, Xu J, Wang J, Wu J, Shi C, et al. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency. Cell. 2017;169:243-257.e25 pubmed publisher
  • immunocytochemistry; mouse; 1:200; fig 2
Klose R, Krzywinska E, Castells M, Gotthardt D, Putz E, Kantari Mimoun C, et al. Targeting VEGF-A in myeloid cells enhances natural killer cell responses to chemotherapy and ameliorates cachexia. Nat Commun. 2016;7:12528 pubmed publisher
  • immunocytochemistry; human; fig 6
Pijuan Galitó S, Tamm C, Schuster J, Sobol M, Forsberg L, Merry C, et al. Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture. Nat Commun. 2016;7:12170 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig 1d
Zhang Z, Chen Y, Zhang T, Guo L, Yang W, Zhang J, et al. Role of Myoendothelial Gap Junctions in the Regulation of Human Coronary Artery Smooth Muscle Cell Differentiation by Laminar Shear Stress. Cell Physiol Biochem. 2016;39:423-37 pubmed publisher
  • immunohistochemistry - paraffin section; human; 1:100; fig 3
Batchelder C, Martinez M, Tarantal A. Natural Scaffolds for Renal Differentiation of Human Embryonic Stem Cells for Kidney Tissue Engineering. PLoS ONE. 2015;10:e0143849 pubmed publisher
  • western blot; mouse; 1:1000; fig s5
Kyathanahalli C, Organ K, Moreci R, Anamthathmakula P, Hassan S, Caritis S, et al. Uterine endoplasmic reticulum stress-unfolded protein response regulation of gestational length is caspase-3 and -7-dependent. Proc Natl Acad Sci U S A. 2015;112:14090-5 pubmed publisher
  • immunocytochemistry; African green monkey; 1:1500; fig 2s1
Gallego Romero I, Pavlovic B, Hernando Herraez I, Zhou X, WARD M, Banovich N, et al. A panel of induced pluripotent stem cells from chimpanzees: a resource for comparative functional genomics. elife. 2015;4:e07103 pubmed publisher
  • immunocytochemistry; human; 1:1500
Thomas S, Kagan C, Pavlovic B, Burnett J, Patterson K, Pritchard J, et al. Reprogramming LCLs to iPSCs Results in Recovery of Donor-Specific Gene Expression Signature. PLoS Genet. 2015;11:e1005216 pubmed publisher
  • immunocytochemistry; rat; 1:1000; fig 1
  • western blot; rat; fig 2
Zhang Z, Zhang T, Zhou Y, Wei X, Zhu J, Zhang J, et al. Activated phosphatidylinositol 3-kinase/Akt inhibits the transition of endothelial progenitor cells to mesenchymal cells by regulating the forkhead box subgroup O-3a signaling. Cell Physiol Biochem. 2015;35:1643-53 pubmed publisher
  • immunocytochemistry; human; 1:400
Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, et al. Transcription activator-like effector nuclease (TALEN)-mediated CLYBL targeting enables enhanced transgene expression and one-step generation of dual reporter human induced pluripotent stem cell (iPSC) and neural stem cell (NSC) lines. PLoS ONE. 2015;10:e0116032 pubmed publisher
  • immunocytochemistry; human; 1:1500
Patel P, Khan N, Rani M, Gupta D, Jameel S. The expression of HIV-1 Vpu in monocytes causes increased secretion of TGF-? that activates profibrogenic genes in hepatic stellate cells. PLoS ONE. 2014;9:e88934 pubmed publisher
Er E, Valiente M, Ganesh K, Zou Y, Agrawal S, Hu J, et al. Pericyte-like spreading by disseminated cancer cells activates YAP and MRTF for metastatic colonization. Nat Cell Biol. 2018;20:966-978 pubmed publisher
Walker J, Bleaken B, Romisher A, Alnwibit A, Menko A. In wound repair vimentin mediates the transition of mesenchymal leader cells to a myofibroblast phenotype. Mol Biol Cell. 2018;29:1555-1570 pubmed publisher
Semeniak D, Kulawig R, Stegner D, Meyer I, Schwiebert S, Bösing H, et al. Proplatelet formation is selectively inhibited by collagen type I through Syk-independent GPVI signaling. J Cell Sci. 2016;129:3473-84 pubmed publisher
Leng L, Tan Y, Gong F, Hu L, Ouyang Q, Zhao Y, et al. Differentiation of primordial germ cells from induced pluripotent stem cells of primary ovarian insufficiency. Hum Reprod. 2015;30:737-48 pubmed publisher
Yu K, Wang Y, Wang L, Jian Y, Zhao X, Chen J, et al. Mitochondrial KATP channel involvement in angiotensin II-induced autophagy in vascular smooth muscle cells. Basic Res Cardiol. 2014;109:416 pubmed publisher
Boreström C, Simonsson S, Enochson L, Bigdeli N, Brantsing C, Ellerstrom C, et al. Footprint-free human induced pluripotent stem cells from articular cartilage with redifferentiation capacity: a first step toward a clinical-grade cell source. Stem Cells Transl Med. 2014;3:433-47 pubmed publisher
Zhang J, Zhang Z, Zhang D, Zhu J, Zhang T, Wang C. microRNA 126 inhibits the transition of endothelial progenitor cells to mesenchymal cells via the PIK3R2-PI3K/Akt signalling pathway. PLoS ONE. 2013;8:e83294 pubmed publisher
Loh K, Ng G, Yu C, Fhu C, Yu D, Vennekens R, et al. TRPM4 inhibition promotes angiogenesis after ischemic stroke. Pflugers Arch. 2014;466:563-76 pubmed publisher
Negrão R, Duarte D, Costa R, Soares R. Isoxanthohumol modulates angiogenesis and inflammation via vascular endothelial growth factor receptor, tumor necrosis factor alpha and nuclear factor kappa B pathways. Biofactors. 2013;39:608-22 pubmed publisher
Chang W, Lavoie J, Kwon S, Chen Z, Manias J, Behbahani J, et al. Feeder-independent derivation of induced-pluripotent stem cells from peripheral blood endothelial progenitor cells. Stem Cell Res. 2013;10:195-202 pubmed publisher
Kiefer S, Robbins L, Rauchman M. Conditional expression of Wnt9b in Six2-positive cells disrupts stomach and kidney function. PLoS ONE. 2012;7:e43098 pubmed publisher
Chan K, Fu S, Hui W, Chan L, Rui Y, Qin L, et al. Radix Dipsaci does not improve tendon healing in a rat model of patellar tendon donor site injury. Orthop Surg. 2010;2:187-93 pubmed publisher
Nakayama K, Batchelder C, Lee C, Tarantal A. Renal tissue engineering with decellularized rhesus monkey kidneys: age-related differences. Tissue Eng Part A. 2011;17:2891-901 pubmed publisher
Ben Nun I, Montague S, Houck M, Tran H, Garitaonandia I, Leonardo T, et al. Induced pluripotent stem cells from highly endangered species. Nat Methods. 2011;8:829-31 pubmed publisher
Schutzer W, Beard D, Reed J, Mader S. Characterization of clonal vascular smooth muscle cell lines derived from young and old Fischer 344 rats. In Vitro Cell Dev Biol Anim. 2011;47:445-50 pubmed publisher
Diaz R, Quiles M, Guillem Marti J, Lopez Cano M, Huguet P, Ramon Y Cajal S, et al. Apoptosis-like cell death induction and aberrant fibroblast properties in human incisional hernia fascia. Am J Pathol. 2011;178:2641-53 pubmed publisher
Sakamaki Y, Sakatsume M, Wang X, Inomata S, Yamamoto T, Gejyo F, et al. Injured kidney cells express SM22? (transgelin): Unique features distinct from ?-smooth muscle actin (?SMA). Nephrology (Carlton). 2011;16:211-8 pubmed publisher
Mirastschijski U, Schnabel R, Claes J, Schneider W, AGren M, Haaksma C, et al. Matrix metalloproteinase inhibition delays wound healing and blocks the latent transforming growth factor-beta1-promoted myofibroblast formation and function. Wound Repair Regen. 2010;18:223-34 pubmed publisher
Graumann U, Ritz M, Rivero B, Hausmann O. CD133 expressing pericytes and relationship to SDF-1 and CXCR4 in spinal cord injury. Curr Neurovasc Res. 2010;7:144-54 pubmed
Gerlach J, Lübberstedt M, Edsbagge J, Ring A, Hout M, Baun M, et al. Interwoven four-compartment capillary membrane technology for three-dimensional perfusion with decentralized mass exchange to scale up embryonic stem cell culture. Cells Tissues Organs. 2010;192:39-49 pubmed publisher
Hall B, Zheng C, Swaim W, Cho A, Nagineni C, Eckhaus M, et al. Conditional overexpression of TGF-beta1 disrupts mouse salivary gland development and function. Lab Invest. 2010;90:543-55 pubmed publisher
Wempe F, De Zolt S, Koli K, Bangsow T, Parajuli N, Dumitrascu R, et al. Inactivation of sestrin 2 induces TGF-beta signaling and partially rescues pulmonary emphysema in a mouse model of COPD. Dis Model Mech. 2010;3:246-53 pubmed publisher
Rowland T, Miller L, Blaschke A, Doss E, Bonham A, Hikita S, et al. Roles of integrins in human induced pluripotent stem cell growth on Matrigel and vitronectin. Stem Cells Dev. 2010;19:1231-40 pubmed publisher
Gerlach J, Hout M, Edsbagge J, Bjorquist P, Lübberstedt M, Miki T, et al. Dynamic 3D culture promotes spontaneous embryonic stem cell differentiation in vitro. Tissue Eng Part C Methods. 2010;16:115-21 pubmed publisher
Shao L, Feng W, Sun Y, Bai H, Liu J, Currie C, et al. Generation of iPS cells using defined factors linked via the self-cleaving 2A sequences in a single open reading frame. Cell Res. 2009;19:296-306 pubmed publisher
Jeyasuria P, Wetzel J, Bradley M, Subedi K, Condon J. Progesterone-regulated caspase 3 action in the mouse may play a role in uterine quiescence during pregnancy through fragmentation of uterine myocyte contractile proteins. Biol Reprod. 2009;80:928-34 pubmed publisher
Liu C, Sun M, Yan X, Han L, Zhang Y, Liu C, et al. Inhibition of hepatic stellate cell activation following Yinchenhao decoction administration to dimethylnitrosamine-treated rats. Hepatol Res. 2008;38:919-29 pubmed publisher
Zvereff V, Wang J, Shun K, Lacoste J, Chevrette M. Colocalisation of CD9 and mortalin in CD9-induced mitotic catastrophe in human prostate cancer cells. Br J Cancer. 2007;97:941-8 pubmed
Koli K, Myllärniemi M, Vuorinen K, Salmenkivi K, Ryynänen M, Kinnula V, et al. Bone morphogenetic protein-4 inhibitor gremlin is overexpressed in idiopathic pulmonary fibrosis. Am J Pathol. 2006;169:61-71 pubmed
Zvieriev V, Wang J, Chevrette M. Over-expression of CD9 does not affect in vivo tumorigenic or metastatic properties of human prostate cancer cells. Biochem Biophys Res Commun. 2005;337:498-504 pubmed
product information
Catalog Number :
CBL171
Subcategory :
Cell Structure
Product Name :
Anti-Actin Antibody, smooth muscle, clone ASM-1
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
P62736
Host Name :
Mouse
Antigen :
Actin
Clone :
ASM-1
Conjugate :
Purified
Isotype :
IgG2a
Product Description :
Anti-Actin Antibody, smooth muscle, clone ASM-1
Cross Reactivity :
Bovine;Chicken;Horse;Human;Mouse;Rat
Background :
ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. This antibody reacts with many types of smooth muscle cells, such as those present in vascular walls, intestinal muscularis mucosae and propria, myometrium, stroma of various tissues, and is also positive for myoepithelial cells of various glands, notably salivary and mammary gland. Myogenic soft tissues detected include leiomyosarcomas, leiomyomas, and certain stromal cells surrounding infiltrating ductal carcinoma of the breast.
ALT Names :
Alpha-actin-2
Immunogen :
Synthetic peptide corresponding to the ten N-terminal amino acids of the alpha-smooth muscle isoform of actin.
Specificity :
This antibody is specific for the alpha-smooth-muscle isoform of actin (MW 43 kDa).
Package Size :
50 µg
Uses :
Immunofluorescence;Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting
Storage :
Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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