CD3 Antibody, clone UCHT1 | EMD Millipore CBL150 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

CD3 Antibody, clone UCHT1

catalog :

CBL150

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

UCHT1

The same clone is also sold as:

Abnova: MAB13727, MAB6007, MAB4402, MAB6006, MAB12204, MAB6008, MAB6009, MAB6010, MAB6004, MAB9817, MAB9816
Bio-Rad: MCA463PE, MCA463A647, MCA463SBV515, MCA463GT, MCA463C, MCA463A488, MCA463F, MCA463PET, MCA463A647T, MCA463PECY5.5, MCA463FT, MCA463EL, MCA463APC, MCA463A700, MCA463P750, MCA463PB, MCA463G, MCA463P647
R&D Systems: FAB100A-100, FAB100T-025, FAB100C-100, FAB100P-100, FAB100S-025, FAB100R-100UG, FAB100V-025, FAB100N-100, FAB100U-100UG, FAB100G-025, MAB100-100, FAB100F-100
LifeSpan Biosciences: LS-C35367, LS-C78118, LS-C357686
Abcam: ab22
Rockland Immunochemicals: 200-302-N14, 200-308-N14, 200-326-N14
Invitrogen: 47-0038-42, 47-0038-41, 606-0038-42, 13-0038-80, MA1-21454, MA1-80044, CD0328, 13-0038-82, 48-0038-41, 48-0038-42, MA1-80537, 67-0038-42, CD0330, 15-0038-42, 15-0038-41, MA1-10178, 64-0038-41, 16-0038-025, 606-0038-41, CD0305, 63-0038-42, 61-0038-42, 62-0038-42, CD0329, 17-0038-41, 64-0038-42, 14-0038-80, 78-0038-42, 16-0038-050, 48-0038-82, 56-0038-42, Q10054, MA1-10179, A15440, 16-0038-85, 16-0038-81, MA1-10177, CD0330TR, 69-0038-41, 25-0038-41, 11-0038-80, A51001, GM4013-5, 58-0038-42, CD0304, 58-0038-41, CD0301, 25-0038-42, MA1-10181, 56-0038-82, 56-0038-80, 14-0038-82, 17-0038-42, 12-0038-42, 62-0038-41, 11-0038-41, 11-0038-42, 16-0038-38, CD0326, 69-0038-42, 12-0038-41, 505-0038-42, 505-0038-41
Absolute Antibody: Ab00112-10.0, Ab00112-1.1, Ab00112-23.0, Ab00112-1.4, Ab00112-10.3
ichorbio: ICH1002
Santa Cruz Biotechnology: sc-1179 HRP, sc-1179, sc-1179 AC
BioLegend: 300483, 300440, 300426, 300433, 300481, 300406, 300420, 300439, 300434, 300438, 300443, 300410, 300469, 300470, 300408, 300428, 300430, 300459, 300424, 300407, 300419, 300415, 300454, 300468, 300427, 300482, 300423, 300431, 300452, 300442, 300441, 300460, 300429, 300422, 300425, 300473, 300474, 300464, 300449, 300405, 300435, 300436, 300466, 300416, 300448, 300437, 300418, 300458, 300450, 300402, 300471, 300447, 300446, 300417, 300463, 300465, 300475, 300401, 300472, 300404, 300411, 300456, 300467, 300479, 300403, 300477, 300412
BD Biosciences: 560835, 563546, 562280, 561810, 563725, 557943, 561812, 555334, 563423, 563724, 561007, 564466, 560366, 555329, 561417, 555331, 555330, 560365, 557694, 564465, 555333, 561811, 555332, 561806, 562426, 561416, 561807, 555916, 561027, 550368, 557706, 558117
OriGene: TA807198S, SM1057AF, SM1057F, TA807198, SM1057PP, TA320273, CF807198, SM1057RT, SM1057B, SM1057FT, SM1057LE, SM1057P, SM1057R, SM1057APC, SM1057PT
MilliporeSigma: P5810, C7048, F0522
Exbio: T9-514-T025, PB-514-T025, 11-514-C100, 1B-514-C100, PB-514-T100, 11-514-C025, 1A-514-T025, T4-514-T100, 1P-514-T100, 1F-514-T025, T7-514-T025, T9-514-T100, PC-514-T100, PO-514-T025, 1B-514-C025, 12-514-C100, T4-514-T025, 1P-514-T025, PC-514-T025, T8-514-T025, T7-514-T100, 1A-514-T100, T8-514-T100, 1F-514-T100, PO-514-T100
Novus Biologicals: NB100-2726, NBP2-80638

reactivity :

human

application :

immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry

citations: 12

Reference
Rodríguez Fdez S, Citterio C, Lorenzo Martín L, Baltanás Copado J, Llorente Gonzalez C, Corbalan Garcia S, et al. Phosphatidylinositol Monophosphates Regulate Optimal Vav1 Signaling Output. Cells. 2019;8: pubmed publisher
Ngai H, Tian G, Courtney A, Ravari S, Guo L, Liu B, et al. IL-21 Selectively Protects CD62L+ NKT Cells and Enhances Their Effector Functions for Adoptive Immunotherapy. J Immunol. 2018;201:2141-2153 pubmed publisher
Zhao M, Tan Y, Peng Q, Huang C, Guo Y, Liang G, et al. IL-6/STAT3 pathway induced deficiency of RFX1 contributes to Th17-dependent autoimmune diseases via epigenetic regulation. Nat Commun. 2018;9:583 pubmed publisher
Abate F, da Silva Almeida A, Zairis S, Robles Valero J, Couronné L, Khiabanian H, et al. Activating mutations and translocations in the guanine exchange factor VAV1 in peripheral T-cell lymphomas. Proc Natl Acad Sci U S A. 2017;114:764-769 pubmed publisher
Yurugi H, Tanida S, Akita K, Ishida A, Toda M, Nakada H. Prohibitins function as endogenous ligands for Siglec-9 and negatively regulate TCR signaling upon ligation. Biochem Biophys Res Commun. 2013;434:376-81 pubmed publisher
Teigler J, Iampietro M, Barouch D. Vaccination with adenovirus serotypes 35, 26, and 48 elicits higher levels of innate cytokine responses than adenovirus serotype 5 in rhesus monkeys. J Virol. 2012;86:9590-8 pubmed publisher
Yurugi H, Tanida S, Ishida A, Akita K, Toda M, Inoue M, et al. Expression of prohibitins on the surface of activated T cells. Biochem Biophys Res Commun. 2012;420:275-80 pubmed publisher
Nikolic D, Lehmann M, Felts R, Garcia E, Blanchet F, Subramaniam S, et al. HIV-1 activates Cdc42 and induces membrane extensions in immature dendritic cells to facilitate cell-to-cell virus propagation. Blood. 2011;118:4841-52 pubmed publisher
Mei H, Frölich D, Giesecke C, Loddenkemper C, Reiter K, Schmidt S, et al. Steady-state generation of mucosal IgA+ plasmablasts is not abrogated by B-cell depletion therapy with rituximab. Blood. 2010;116:5181-90 pubmed publisher
Darke C, Coates E. One-tube HLA-B27/B2708 typing by flow cytometry using two "Anti-HLA-B27" monoclonal antibody reagents. Cytometry B Clin Cytom. 2010;78:21-30 pubmed publisher
Woo J, Liu J, Kang S, Singh R, Park S, Su Y, et al. GMP production and characterization of the bivalent anti-human T cell immunotoxin, A-dmDT390-bisFv(UCHT1) for phase I/II clinical trials. Protein Expr Purif. 2008;58:1-11 pubmed
Chan J, Hyduk S, Cybulsky M. Chemoattractants induce a rapid and transient upregulation of monocyte alpha4 integrin affinity for vascular cell adhesion molecule 1 which mediates arrest: an early step in the process of emigration. J Exp Med. 2001;193:1149-58 pubmed

product information

Catalog Number :

CBL150

Subcategory :

Inflammation & Immunology

Product Name :

Anti-CD3 Antibody, clone UCHT1

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P07766

Host Name :

Mouse

Antigen :

CD3

Clone :

UCHT1

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-CD3 Antibody, clone UCHT1

Cross Reactivity :

Human

ALT Names :

TCR

Immunogen :

Purified human T cells

Specificity :

This antibody recognizes 22/26/30 kDa molecular weight lymphocyte surface molecules associated with the T cell antigen receptor complex. CD3 is closely associated on the lymphocyte cell surface with the T cell antigen receptor TcR (2-4). It is believed that the CD3 complex transmits activational signals to the T cell's interior upon antigen recognition by TcR . CD45 (LCA), a protein tyrosine phosphatase, is a potent regulator of signal transduction by the CD3 complex. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, S zary leukaemias and approximately 70% of acute lymphoblastic leukaemias of T cell origin. Antigen distribution: T cells 100% Peripheral Blood lymphocytes 75 + 7% Thymocytes 68 + 5% Granulocytes 1% Monocytes 1% B cells (E-) 1% NK cells (CD16+) 1% KNOWN SPECIES CROSS REACTIVITY: Negative with rhesus monkey peripheral blood leucocytes in flow cytometry FUSION PARTNER: P3 x 63 Ag8.653 myeloma cell line

Package Size :

100 µg

Uses :

Flow Cytometry;Immunofluorescence;Immunoprecipitation;Affects Function;Immunohistochemistry

Storage :

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.