GAPDH Mouse mAb (6C5) | EMD Millipore CB1001-500UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

GAPDH Mouse mAb (6C5)

catalog :

CB1001-500UG

quantity :

500 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs

application :

western blot, ELISA, immunocytochemistry

citations: 21

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1500; loading ...; fig s8c	Mukhtar T, Breda J, Grison A, Karimaddini Z, Grobecker P, Iber D, et al. Tead transcription factors differentially regulate cortical development. Sci Rep. 2020;10:4625 pubmed publisher
immunocytochemistry; mouse; 1:1000; loading ...; fig s2g	Moradi M, Sivadasan R, Saal L, LÃ¼ningschrÃ¶r P, Dombert B, Rathod R, et al. Differential roles of Î±-, Î²-, and Î³-actin in axon growth and collateral branch formation in motoneurons. J Cell Biol. 2017;216:793-814 pubmed publisher
western blot; mouse; 1:4000; fig 13	Schmitt D, Funk N, Blum R, Asan E, Andersen L, Rülicke T, et al. Initial characterization of a Syap1 knock-out mouse and distribution of Syap1 in mouse brain and cultured motoneurons. Histochem Cell Biol. 2016;146:489-512 pubmed publisher
western blot; rat; 1:10,000	Mavlyutov T, Duellman T, Kim H, Epstein M, Leese C, Davletov B, et al. Sigma-1 receptor expression in the dorsal root ganglion: Reexamination using a highly specific antibody. Neuroscience. 2016;331:148-57 pubmed publisher
western blot; human; fig 4	Freund P, Porpaczy E, Le T, Gruber M, Pausz C, Staber P, et al. Cannabinoid Receptors Are Overexpressed in CLL but of Limited Potential for Therapeutic Exploitation. PLoS ONE. 2016;11:e0156693 pubmed publisher
western blot; dogs; 1:1000; fig 1d	Gray M, Lee S, McDowell A, Erskine M, Loh Q, Grice O, et al. Dual targeting of EGFR and ERBB2 pathways produces a synergistic effect on cancer cell proliferation and migration in vitro. Vet Comp Oncol. 2017;15:890-909 pubmed publisher
western blot; human; fig 4	Jennewein L, Ronellenfitsch M, Antonietti P, Ilina E, Jung J, Stadel D, et al. Diagnostic and clinical relevance of the autophago-lysosomal network in human gliomas. Oncotarget. 2016;7:20016-32 pubmed publisher
western blot; human; 1:20,000; fig 3 western blot; mouse; 1:20,000; fig 5	Takabatake Y, Oxvig C, Nagi C, Adelson K, Jaffer S, Schmidt H, et al. Lactation opposes pappalysin-1-driven pregnancy-associated breast cancer. EMBO Mol Med. 2016;8:388-406 pubmed publisher
western blot; human; 1:1000; fig 1	Jia L, Li B, Yu H. The Bub1-Plk1 kinase complex promotes spindle checkpoint signalling through Cdc20 phosphorylation. Nat Commun. 2016;7:10818 pubmed publisher
western blot; human; 1:2500; fig 2	Jansen J, Timal S, van Scherpenzeel M, Michelakakis H, Vicogne D, Ashikov A, et al. TMEM199 Deficiency Is a Disorder of Golgi Homeostasis Characterized by Elevated Aminotransferases, Alkaline Phosphatase, and Cholesterol and Abnormal Glycosylation. Am J Hum Genet. 2016;98:322-30 pubmed publisher
western blot; mouse; fig 1 western blot; human; fig 1	Abu Odeh M, Hereema N, Aqeilan R. WWOX modulates the ATR-mediated DNA damage checkpoint response. Oncotarget. 2016;7:4344-55 pubmed publisher
western blot; mouse; 1:5000	Kraushar M, Viljetić B, Wijeratne H, Thompson K, Jiao X, Pike J, et al. Thalamic WNT3 Secretion Spatiotemporally Regulates the Neocortical Ribosome Signature and mRNA Translation to Specify Neocortical Cell Subtypes. J Neurosci. 2015;35:10911-26 pubmed publisher
western blot; mouse; fig 2	Liechtenstein T, Perez Janices N, Blanco Luquin I, Goyvaerts C, Schwarze J, Dufait I, et al. Anti-melanoma vaccines engineered to simultaneously modulate cytokine priming and silence PD-L1 characterized using ex vivo myeloid-derived suppressor cells as a readout of therapeutic efficacy. Oncoimmunology. 2014;3:e945378 pubmed
western blot; human; 1:1000; fig 3	Gennarino V, Singh R, White J, De Maio A, Han K, Kim J, et al. Pumilio1 haploinsufficiency leads to SCA1-like neurodegeneration by increasing wild-type Ataxin1 levels. Cell. 2015;160:1087-98 pubmed publisher
western blot; human; fig 6	Guerrero A, Iglesias C, Raguz S, Floridia E, Gil J, Pombo C, et al. The cerebral cavernous malformation 3 gene is necessary for senescence induction. Aging Cell. 2015;14:274-83 pubmed publisher
western blot; mouse; 1:10,000; fig 4	Kurz A, Double K, Lastres Becker I, Tozzi A, Tantucci M, Bockhart V, et al. A53T-alpha-synuclein overexpression impairs dopamine signaling and striatal synaptic plasticity in old mice. PLoS ONE. 2010;5:e11464 pubmed publisher
	Damiano Guercio J, Kurzawa L, Mueller J, Dimchev G, Schaks M, Nemethova M, et al. Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion. elife. 2020;9: pubmed publisher
	Li D, Musante V, Zhou W, Picciotto M, Nairn A. Striatin-1 is a B subunit of protein phosphatase PP2A that regulates dendritic arborization and spine development in striatal neurons. J Biol Chem. 2018;293:11179-11194 pubmed publisher
	Gennarino V, Palmer E, McDonell L, Wang L, Adamski C, Koire A, et al. A Mild PUM1 Mutation Is Associated with Adult-Onset Ataxia, whereas Haploinsufficiency Causes Developmental Delay and Seizures. Cell. 2018;172:924-936.e11 pubmed publisher
	Zhu L, Yang J, Bromberger T, Holly A, Lu F, Liu H, et al. Structure of Rap1b bound to talin reveals a pathway for triggering integrin activation. Nat Commun. 2017;8:1744 pubmed publisher
	Gennarino V, Alcott C, Chen C, Chaudhury A, Gillentine M, Rosenfeld J, et al. NUDT21-spanning CNVs lead to neuropsychiatric disease and altered MeCP2 abundance via alternative polyadenylation. elife. 2015;4: pubmed publisher

product information

Catalog Number :

CB1001-500UG

Category :

Antibodies

Subcategory :

Cell Structure

Product Name :

Anti-GAPDH Mouse mAb (6C5)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

2597 (Hu), 100043349 (Ms), 500983 689689 24383 685186 (Rt)

Host Name :

Clone :

6C5

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-GAPDH Mouse mAb (6C5)

Cross Reactivity :

Canine, Chicken, Fish, Frog, Human, Mouse, Porcine, Rabbit, Rat

Background :

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is one of the key enzymes involved in glycolysis. GAPDH is comprised of four ~36 kDa subunits with a total molecular weight of ~146 kDa. Besides functioning as a glycolytic enzyme in cytoplasm, mammalian GAPDH is also involved in a great number of intracellular processes such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication and DNA repair. Recently, findings have been made concerning the role of GAPDH in differentpathologies including prostate cancer progression, programmed neuronal cell death, and age-related neuronal diseases such as Alzheimer’s Disease and Huntington’s Disease. While GAPDH is constitutively expressed in almost all tissues at high levels, there are some physiological factors such as hypoxia and diabetes that increase GAPDH expression in certain cell types.

ALT Names :

G3PD, G3PDH, GAPD, Gapdh, Glyceraldehyde-3-phosphate Dehydrogenase, NUCLEAR URACIL DNA GLYCOSYLASE, glyceraldehyde-3-phosphate dehydrogenase

Immunogen :

purified, rabbit muscle

Specificity :

Recognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.

Package Size :

500 ug

Uses :

ELISA, WB, IC

Storage :

Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.