PhosphoDetect™ Anti-PDH-E1alpha (pSer300) Rabbit pAb | EMD Millipore AP1064-50UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PhosphoDetect™ Anti-PDH-E1alpha (pSer300) Rabbit pAb

catalog :

AP1064-50UG

quantity :

50 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

application :

western blot, immunocytochemistry

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 3e	Titov D, Cracan V, Goodman R, Peng J, Grabarek Z, Mootha V. Complementation of mitochondrial electron transport chain by manipulation of the NAD+/NADH ratio. Science. 2016;352:231-5 pubmed publisher

product information

Catalog Number :

AP1064-50UG

Category :

Antibodies

Subcategory :

Cell Signaling

Product Name :

PhosphoDetect™ Anti-PDH-E1alpha (pSer300) Rabbit pAb

Product Type :

Antibodies

Clonality :

Polyclonal

Host Name :

Conjugate :

Purified

Product Description :

PhosphoDetect™ Anti-PDH-E1alpha (pSer300) Rabbit pAb

Cross Reactivity :

Human, Mouse, Rat

Background :

The pyruvate dehydogenase (PDH) complex (PDC) catalyzes the irreversible conversion of pyruvate to acetyl CoA, providing the link between glycolyis and the TCA cycle. Dysregulation of the PDC in skeletal muscle has been implicated in type 2 diabetes and mutations in the PDC result in various mitochondrial diseases. The PDC is a multienzyme complex composed of several copies of three catalytic proteins (E1, E2, and E3): one structural (E3-binding protein) and two regulatory proteins (PDH kinase [PDK] and PDH phosphatase [PDP]). The activity of the PDC is mainly regulated by phosphorylation-dephosphorylation of three specific serine residues (232, 293, 300) on the E1alpha subunit. Phosphorylation of PDH determines the activity of the complex. Phosphorylation by one of four PDK isoforms inactivates the complex, while removal of the phosphate by one of two PDP isoforms activates the complex.

Immunogen :

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 300 phosphorylation site of human PDH-E1alpha

Specificity :

Recognizes the ~44 kDa PDH-E1alpha protein phosphorylated at Ser 300 .

Package Size :

50 ug

Uses :

WB, IF, IC

Storage :

Following initial thaw, aliquot and freeze (-20°C). Avoid repeated freeze-thaw.