PhosphoDetect™ Anti-PDH-E1alpha (pSer293) Rabbit pAb | EMD Millipore AP1062-50UL product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PhosphoDetect™ Anti-PDH-E1alpha (pSer293) Rabbit pAb

catalog :

AP1062-50UL

quantity :

50 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig s2	Jiang L, Shestov A, Swain P, Yang C, Parker S, Wang Q, et al. Reductive carboxylation supports redox homeostasis during anchorage-independent growth. Nature. 2016;532:255-8 pubmed publisher
western blot; mouse; loading ...; fig 3e	Davidson S, Papagiannakopoulos T, Olenchock B, Heyman J, Keibler M, Luengo A, et al. Environment Impacts the Metabolic Dependencies of Ras-Driven Non-Small Cell Lung Cancer. Cell Metab. 2016;23:517-28 pubmed publisher

product information

Catalog Number :

AP1062-50UL

Category :

Antibodies

Subcategory :

Cell Signaling

Product Name :

PhosphoDetect™ Anti-PDH-E1alpha (pSer293) Rabbit pAb

Product Type :

Antibodies

Clonality :

Polyclonal

Gene ID :

5160 (Hu), 18597 (Ms), 29554 (Rt)

Host Name :

Conjugate :

Purified

Isotype :

IgG

Product Description :

PhosphoDetect™ Anti-PDH-E1alpha (pSer293) Rabbit pAb

Cross Reactivity :

Human, Mouse, Primate, Rat

Background :

Pyruvate dehydogenase (PDH) complex (PDC) catalyzes the irreversible conversion of pyruvate to acetyl CoA, providing the link between glycolyis and the TCA cycle. Dysregulation of the PDC in skeletal muscle has been implicated in type 2 diabetes and mutations in the PDC result in various mitochondrial diseases. The PDC is a multienzyme complex composed of several copies of three catalytic proteins (E1, E2, and E3): one structural (E3-binding protein) and two regulatory proteins (PDH kinase [PDK] and PDH phosphatase [PDP]). The activity of the PDC complex is mainly regulated by phosphorylation-dehosphorylation of three specific serine residues (232, 293, 300) on the E1 subunit. Phosphorylation of PDH determines the activity of the complex. Phosphorylation by one of four PDK isoforms inactivates the complex, while removal of the phosphate by one of two PDP isoforms activates the complex.

ALT Names :

E1 ALPHA PDH, MGC114215, MGC94854, PDHA, Pdha1, PDHCE1A, Pdh e1alpha, PHE1A, Pyruvate dehydrogenase e1 alpha1, pyruvate dehydrogenase (lipoamide) alpha 1

Immunogen :

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser 293 phosphorylation site of human PDH-E1alpha

Specificity :

Recognizes the ~44 kDa PDH-E1alpha protein phosphorylated at Ser 293 .

Package Size :

50 ul

Uses :

WB, IC, IP

Storage :

Following initial thaw, aliquot and freeze (-20°C). Avoid repeated freeze-thaw.