Caspase-3 (Ab-4) Mouse mAb (AM1.31-11) | EMD Millipore AM65-100UG product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Caspase-3 (Ab-4) Mouse mAb (AM1.31-11)

catalog :

AM65-100UG

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

Catalog Number :

AM65-100UG

Category :

Antibodies

Subcategory :

Apoptosis & Cancer

Product Name :

Anti-Caspase-3 (Ab-4) Mouse mAb (AM1.31-11)

Product Type :

Antibodies

Clonality :

Monoclonal

Gene ID :

836 (Hu), 12367 (Ms), 25402 (Rt)

Host Name :

Clone :

AM1.31-11

Conjugate :

Purified

Isotype :

IgG2a

Product Description :

Anti-Caspase-3 (Ab-4) Mouse mAb (AM1.31-11)

Cross Reactivity :

Human, Mouse

Background :

Caspase-3 (CPP32/Yama/Apopain) exists as a ~32 kDa inactive precursor protein in the cytoplasm of most cell types. Upon proteolytic activation the protein is cleaved at a specific site within the molecule to yield a heterodimer consisting of p20 and p11 subunits. The cleavage/activation proceeds in two steps yielding first a p3 fragment followed by the p20 and p11 fragments and is mediated by specific proteases including serine proteases and the enzyme granzyme B. Activation of Caspase-3 occurs rather rapidly following the onset of apoptosis induced by a number of different signals including but not restricted to TGFbeta-1, TNF, and Fas. One of the most potent chemical activators of Caspase-3 is staurosporine. Once activated, Caspase-3 has a number of substrates itself. Among those which have been reported are MDM2, PITSLRE kinases, actin, DNA-PK, and PARP. Related to the C. elegans protein, Ced-3, Caspase-3 has been mapped in humans to chromosome 4q33-q35.

ALT Names :

Apopain, CASPASE-3, CASPASE-3 p20, CC3, CPP32, CPP32B, Cpp32beta, Ice-like cysteine protease, Lice, MGC93645, mldy, P17, PROCASPASE 3, SCA-1, YAMA, caspase 3, apoptosis-related cysteine peptidase

Immunogen :

recombinant human caspase-3

Specificity :

Recognizes the ~11 kDa active subunit of caspase-3.

Package Size :

100 ug

Uses :

WB, IH(P)

Storage :

Following initial thaw, aliquot and freeze (-20°C). Avoid repeated freeze-thaw.