Fluoro-Jade® C | EMD Millipore AG325 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

secondary antibody

product name :

Fluoro-Jade® C

catalog :

AG325

quantity :

50 mg

citations: 23

Reference
Douglas J, Gardner L, Salapa H, Lalor S, Lee S, Segal B, et al. Antibodies to the RNA-binding protein hnRNP A1 contribute to neurodegeneration in a model of central nervous system autoimmune inflammatory disease. J Neuroinflammation. 2016;13:178 pubmed publisher
Ophelders D, Gussenhoven R, Lammens M, Kusters B, Kemp M, Newnham J, et al. Neuroinflammation and structural injury of the fetal ovine brain following intra-amniotic Candida albicans exposure. J Neuroinflammation. 2016;13:29 pubmed publisher
Pikhovych A, Stolberg N, Jessica Flitsch L, Walter H, Graf R, Fink G, et al. Transcranial Direct Current Stimulation Modulates Neurogenesis and Microglia Activation in the Mouse Brain. Stem Cells Int. 2016;2016:2715196 pubmed publisher
Lin H, Narasimhan P, Liu S, Chan P, Lai I. Postconditioning mitigates cell death following oxygen and glucose deprivation in PC12 cells and forebrain reperfusion injury in rats. J Neurosci Res. 2015;93:140-8 pubmed publisher
Meadowcroft M, Connor J, Yang Q. Cortical iron regulation and inflammatory response in Alzheimer's disease and APPSWE/PS1ΔE9 mice: a histological perspective. Front Neurosci. 2015;9:255 pubmed publisher
Wang R, Ma W, Gao G, Mao Q, Zheng J, Sun L, et al. Fluoro jade-C staining in the assessment of brain injury after deep hypothermia circulatory arrest. Brain Res. 2011;1372:127-32 pubmed publisher
Rohn T, Vyas V, Hernandez-Estrada T, Nichol K, Christie L, Head E. Lack of pathology in a triple transgenic mouse model of Alzheimer's disease after overexpression of the anti-apoptotic protein Bcl-2. J Neurosci. 2008;28:3051-9 pubmed publisher
Elias P, Spector M. Characterization of a bilateral penetrating brain injury in rats and evaluation of a collagen biomaterial for potential treatment. J Neurotrauma. 2012;29:2086-102 pubmed publisher
Krstic D, Madhusudan A, Doehner J, Vogel P, Notter T, Imhof C, et al. Systemic immune challenges trigger and drive Alzheimer-like neuropathology in mice. J Neuroinflammation. 2012;9:151 pubmed publisher
Ravikumar M, Jain S, Miller R, Capadona J, Selkirk S. An organotypic spinal cord slice culture model to quantify neurodegeneration. J Neurosci Methods. 2012;211:280-8 pubmed publisher
Ding Y, Xia Y, Jiao X, Duan L, Yu J, Wang X, et al. The TrkB-positive dopaminergic neurons are less sensitive to MPTP insult in the substantia nigra of adult C57/BL mice. Neurochem Res. 2011;36:1759-66 pubmed publisher
Huang J, Huang K, Shang L, Wang H, Zhang M, Fan C, et al. Chronic lead exposure reduces doublecortin-expressing immature neurons in young adult guinea pig cerebral cortex. BMC Neurosci. 2012;13:82 pubmed publisher
Dhanushkodi A, McDonald M. Intracranial V. cholerae sialidase protects against excitotoxic neurodegeneration. PLoS ONE. 2011;6:e29285 pubmed publisher
Too L, Ball H, McGregor I, Hunt N. The pro-inflammatory cytokine interferon-gamma is an important driver of neuropathology and behavioural sequelae in experimental pneumococcal meningitis. Brain Behav Immun. 2014;40:252-68 pubmed publisher
Zhen J, Wang W, Zhou J, Qu Z, Fang H, Zhao R, et al. Chronic intermittent hypoxic preconditioning suppresses pilocarpine-induced seizures and associated hippocampal neurodegeneration. Brain Res. 2014;1563:122-30 pubmed publisher
Wu T, Li W, Li H. Netrin-1 attenuates ischemic stroke-induced apoptosis. Neuroscience. 2008;156:475-82 pubmed publisher
Shi R, Weng J, Zhao L, Li X, Gao T, Kong J. Excessive autophagy contributes to neuron death in cerebral ischemia. CNS Neurosci Ther. 2012;18:250-60 pubmed publisher
Lazaridis I, Charalampopoulos I, Alexaki V, Avlonitis N, Pediaditakis I, Efstathopoulos P, et al. Neurosteroid dehydroepiandrosterone interacts with nerve growth factor (NGF) receptors, preventing neuronal apoptosis. PLoS Biol. 2011;9:e1001051 pubmed publisher
Chen B, Friedman B, Whitney M, Winkle J, Lei I, Olson E, et al. Thrombin activity associated with neuronal damage during acute focal ischemia. J Neurosci. 2012;32:7622-31 pubmed publisher
Liu Y, Wang L, Wei L, Huang Y, Chen L. Up-regulation of D-serine might induce GABAergic neuronal degeneration in the cerebral cortex and hippocampus in the mouse pilocarpine model of epilepsy. Neurochem Res. 2009;34:1209-18 pubmed publisher
Deng B, Gou X, Chen H, Li L, Zhong H, Xu H, et al. Targeted delivery of neurogenin-2 protein in the treatment for cerebral ischemia-reperfusion injury. Biomaterials. 2013;34:8786-97 pubmed publisher
Mukherjee S, Zeitouni S, Cavarsan C, Shapiro L. Increased seizure susceptibility in mice 30 days after fluid percussion injury. Front Neurol. 2013;4:28 pubmed publisher
Deng B, Xu H, Zhang J, Wang J, Han L, Li L, et al. Neuroprotective effects of sevoflurane against electromagnetic pulse-induced brain injury through inhibition of neuronal oxidative stress and apoptosis. PLoS ONE. 2014;9:e91019 pubmed publisher

product information

Catalog Number :

AG325

Subcategory :

Secondary & Control Antibodies

Product Name :

Fluoro-Jade® C

Product Type :

Antibodies

Antigen :

Fluoro-Jade® C

Product Description :

Fluoro-Jade® C

Background :

Fluoro-Jade C, like its predecessors, Fluoro-Jade and Fluoro-Jade B, were found to stain all degenerating neurons, regardless of specific insult or mechanism of cell death. Therefore, the patterns of neuronal degeneration seen following exposure to either the glutamate agonist, kainic acid, or the inhibitor of mitochondrial respiration, 3-NPA, were the same for all of the Fluoro-Jade dyes. However, there was a qualitative difference in the staining characteristics of the three fluorochromes. Specifically, Fluoro-Jade C exhibited the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localizing not only degenerating nerve cell bodies, but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols. Triple labeling can be accomplished by staining degenerating neurons with Fluoro-Jade C, cell nuclei with DAPI and activated astrocytes with GFAP immunofluorescence. Fluoro-Jade™ is a registered trademark of Histo-Chem, Inc.

Purity :

Silica TLC (acetanitrile/water, 6/4) revealed the presence of two fluorescent spots, presumably corresponding to the di sulphate homologues. The presence of precursors or free fluorescein was not detected.

Package Size :

50 mg

Uses :

Immunohistochemistry

Storage :

The lyophilized powder should be stored well sealed at room temperature, preferable in a desiccator (due to its hygroscopic nature) for up to one year from date of receipt. The liquid stock solution (0.01%) in distilled water can be stored at 2-8°C for up to 3 months. The .0002-.0001% working solution in 0.1% acetic acid should be used within 4 hrs of preparation.