phospho-MYPT1 (Thr696) Antibody | EMD Millipore ABS45 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-MYPT1 (Thr696) Antibody

catalog :

ABS45

quantity :

200 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, dogs, chicken

application :

western blot, immunohistochemistry

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig s3a	Thompson J, Nguyen Q, Singh M, Pavesic M, Nesterenko I, Nelson L, et al. Rho-associated kinase 1 inhibition is synthetically lethal with von Hippel-Lindau deficiency in clear cell renal cell carcinoma. Oncogene. 2017;36:1080-1089 pubmed publisher
immunohistochemistry; mouse; 1:200; fig 2	Laklai H, Miroshnikova Y, Pickup M, Collisson E, Kim G, Barrett A, et al. Genotype tunes pancreatic ductal adenocarcinoma tissue tension to induce matricellular fibrosis and tumor progression. Nat Med. 2016;22:497-505 pubmed publisher
	Muralidharan A, Maddala R, Skiba N, Rao P. Growth Differentiation Factor-15-Induced Contractile Activity and Extracellular Matrix Production in Human Trabecular Meshwork Cells. Invest Ophthalmol Vis Sci. 2016;57:6482-6495 pubmed publisher
	Yasuda T, Tada Y, Tanabe N, Tatsumi K, West J. Rho-kinase inhibition alleviates pulmonary hypertension in transgenic mice expressing a dominant-negative type II bone morphogenetic protein receptor gene. Am J Physiol Lung Cell Mol Physiol. 2011;301:L667-74 pubmed publisher

product information

Catalog Number :

ABS45

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-MYPT1 (Thr696) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

O14974

Host Name :

Rabbit

Antigen :

phospho-MYPT1 (Thr696)

Conjugate :

Affinity Purified

Product Description :

Anti-phospho-MYPT1 (Thr696) Antibody

Cross Reactivity :

Human;Mouse;Primate;Rat;Canine;Xenopus;Horse;Chicken

Background :

Myosin phosphatase target subunit 1 (MYPT1), also called Myosin-binding subunit of Myosin phosphatase, is one of the subunits and an integral component of the Myosin phosphatase. Myosin phosphatase regulates the interaction of Actin and Myosin downstream of the guanosine triphosphatase Rho, which inhibits Myosin phosphatase through the action of Rho-kinase. It is also involved in smooth muscle contraction. Inhibition of Myosin light chain phosphatase results in Ca2+ sensitization of smooth muscle contraction. This inhibition is modulated through phosphorylation of MYPT1 by a ZIP-like kinase, which associates with MYPTI and phosphorylates the inhibitory site in smooth muscle. The phosphorylation of MYPT1 by protein kinase C results in altered dephosphoryation of Myosin by attenuating the binding of protein phosphatase 1 catalytic subunit (PP1c) and the phosphorylated 20 kDa Myosin light chain to MYPT1. PP1c interacts with at least four binding sites on the amino-terminus of MYPT1. MYPT1 is localized on stress fibers, and is distributed close to the cell membrane and at cell-cell contacts to regulate Myosin phosphatase activity.

ALT Names :

Myosin phosphatase target subunit 1;Myosin phosphatase-targeting subunit 1;Protein phosphatase myosin-binding subunit;myosin phosphatase, target subunit 1;protein phosphatase 1, regulatory (inhibitor) subunit 12

Immunogen :

KLH-conjugated linear peptide corresponding to human phosphorylated Thr696 of MYPT1.

Specificity :

This antibody recognizes phosphorylated MYPT1 at Thr696.

Package Size :

200 μg

Uses :

Western Blotting

Storage :

Stable for 1 year at 2-8°C from date of receipt