GAPDH Antibody | EMD Millipore ABS16 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

GAPDH Antibody

catalog :

ABS16

quantity :

100 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot

citations: 30

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig s2a	Ebright R, Lee S, Wittner B, Niederhoffer K, Nicholson B, Bardia A, et al. Deregulation of ribosomal protein expression and translation promotes breast cancer metastasis. Science. 2020;: pubmed publisher
western blot; mouse; loading ...; fig 1d	Collier M, Alderson T, de Villiers C, Nicholls D, Gastall H, Allison T, et al. HspB1 phosphorylation regulates its intramolecular dynamics and mechanosensitive molecular chaperone interaction with filamin C. Sci Adv. 2019;5:eaav8421 pubmed publisher
western blot; human; 1:2000; loading ...; fig s4	Gómez Fernández P, Urtasun A, Paton A, Paton J, Borrego F, Dersh D, et al. Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein Response. Front Immunol. 2018;9:2934 pubmed publisher
western blot; mouse; 1:3000; loading ...; fig 1b	Szappanos D, Tschismarov R, Perlot T, Westermayer S, Fischer K, Platanitis E, et al. The RNA helicase DDX3X is an essential mediator of innate antimicrobial immunity. PLoS Pathog. 2018;14:e1007397 pubmed publisher
western blot; human; fig 1	Nowacka J, Baumgartner C, Pelorosso C, Roth M, Zuber J, Baccarini M. MEK1 is required for the development of NRAS-driven leukemia. Oncotarget. 2016;7:80113-80130 pubmed publisher
western blot; mouse; 1:1000; fig 3	Raguz J, Jerić I, Niault T, Nowacka J, Kuzet S, Rupp C, et al. Epidermal RAF prevents allergic skin disease. elife. 2016;5: pubmed publisher
western blot; human; 1:1000; loading ...; fig 3b	Song J, Wang Y, Teng M, Zhang S, Yin M, Lu J, et al. Cordyceps militaris induces tumor cell death via the caspase?dependent mitochondrial pathway in HepG2 and MCF?7 cells. Mol Med Rep. 2016;13:5132-40 pubmed publisher
western blot; mouse; 1:2000; fig 2	Polanco J, Scicluna B, Hill A, Götz J. Extracellular Vesicles Isolated from the Brains of rTg4510 Mice Seed Tau Protein Aggregation in a Threshold-dependent Manner. J Biol Chem. 2016;291:12445-66 pubmed publisher
western blot; human; 1:1000; fig 4	Liu H, Zhang S, Zhao L, Zhang Y, Li Q, Chai X, et al. Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis. Stem Cells Int. 2016;2016:2524092 pubmed publisher
	Ólafsson E, Ten Hoeve A, Li Wang X, Westermark L, Varas Godoy M, Barragan A. Convergent Met and voltage-gated Ca2+ channel signaling drives hypermigration of Toxoplasma-infected dendritic cells. J Cell Sci. 2020;134: pubmed publisher
	Platanitis E, Demiröz D, Schneller A, Fischer K, Capelle C, Hartl M, et al. A molecular switch from STAT2-IRF9 to ISGF3 underlies interferon-induced gene transcription. Nat Commun. 2019;10:2921 pubmed publisher
	Brayford S, Kenny F, Hiratsuka T, Serna Morales E, Yolland L, Luchici A, et al. Heterotypic contact inhibition of locomotion can drive cell sorting between epithelial and mesenchymal cell populations. J Cell Sci. 2019;132: pubmed publisher
	Sparrow A, Sievert K, Patel S, Chang Y, Broyles C, Brook F, et al. Measurement of Myofilament-Localized Calcium Dynamics in Adult Cardiomyocytes and the Effect of Hypertrophic Cardiomyopathy Mutations. Circ Res. 2019;124:1228-1239 pubmed publisher
	Wild T, Budzowska M, Hellmuth S, Eibes S, Karemore G, Barisic M, et al. Deletion of APC7 or APC16 Allows Proliferation of Human Cells without the Spindle Assembly Checkpoint. Cell Rep. 2018;25:2317-2328.e5 pubmed publisher
	Daga N, Eicher S, Kannan A, Casanova A, Low S, Kreibich S, et al. Growth-restricting effects of siRNA transfections: a largely deterministic combination of off-target binding and hybridization-independent competition. Nucleic Acids Res. 2018;46:9309-9320 pubmed publisher
	Nguyen H, Leong W, Li W, Reddy P, Sullivan J, Walter M, et al. Spliceosome Mutations Induce R Loop-Associated Sensitivity to ATR Inhibition in Myelodysplastic Syndromes. Cancer Res. 2018;78:5363-5374 pubmed publisher
	Wang X, Qu Y, Zhang Y, Li S, Sun Y, Chen Z, et al. Antifatigue Potential Activity of Sarcodon imbricatus in Acute Excise-Treated and Chronic Fatigue Syndrome in Mice via Regulation of Nrf2-Mediated Oxidative Stress. Oxid Med Cell Longev. 2018;2018:9140896 pubmed publisher
	Li C, Götz J. Pyk2 is a Novel Tau Tyrosine Kinase that is Regulated by the Tyrosine Kinase Fyn. J Alzheimers Dis. 2018;64:205-221 pubmed publisher
	Shi J, Surma M, Wei L. Disruption of ROCK1 gene restores autophagic flux and mitigates doxorubicin-induced cardiotoxicity. Oncotarget. 2018;9:12995-13008 pubmed publisher
	Campbell M, Watanabe T, Nakano K, Davis R, Lyu Y, Tepper C, et al. KSHV episomes reveal dynamic chromatin loop formation with domain-specific gene regulation. Nat Commun. 2018;9:49 pubmed publisher
	Maphis N, Jiang S, Binder J, Wright C, Gopalan B, Lamb B, et al. Whole Genome Expression Analysis in a Mouse Model of Tauopathy Identifies MECP2 as a Possible Regulator of Tau Pathology. Front Mol Neurosci. 2017;10:69 pubmed publisher
	Teveroni E, Pellegrino M, Sacconi S, Calandra P, Cascino I, Farioli Vecchioli S, et al. Estrogens enhance myoblast differentiation in facioscapulohumeral muscular dystrophy by antagonizing DUX4 activity. J Clin Invest. 2017;127:1531-1545 pubmed publisher
	Bodea L, Evans H, Van der Jeugd A, Ittner L, Delerue F, Kril J, et al. Accelerated aging exacerbates a pre-existing pathology in a tau transgenic mouse model. Aging Cell. 2017;16:377-386 pubmed publisher
	Henrich C. A Microplate-Based Nonradioactive Protein Synthesis Assay: Application to TRAIL Sensitization by Protein Synthesis Inhibitors. PLoS ONE. 2016;11:e0165192 pubmed publisher
	Li S, Zhang H, Du Z, Li C, An M, Zong Z, et al. Induction of epithelial-mesenchymal transition (EMT) by Beclin 1 knockdown via posttranscriptional upregulation of ZEB1 in thyroid cancer cells. Oncotarget. 2016;7:70364-70377 pubmed publisher
	Jacqueroud L, Bouard C, Richard G, Payen L, Devouassoux Shisheboran M, Spicer D, et al. The Heterodimeric TWIST1-E12 Complex Drives the Oncogenic Potential of TWIST1 in Human Mammary Epithelial Cells. Neoplasia. 2016;18:317-327 pubmed publisher
	Hayward S, Wilson C, Kidambi S. Hyaluronic acid-conjugated liposome nanoparticles for targeted delivery to CD44 overexpressing glioblastoma cells. Oncotarget. 2016;7:34158-71 pubmed publisher
	Sugihara T, Nakagawa S, Sasajima Y, Ichinose T, Hiraike H, Kondo F, et al. Loss of the cell polarity determinant human Discs-large is a novel molecular marker of nodal involvement and poor prognosis in endometrial cancer. Br J Cancer. 2016;114:1012-8 pubmed publisher
	Boyer J, Baron M, Constantin A, DegboÃ© Y, Cantagrel A, Davignon J. Anti-TNF certolizumab pegol induces antioxidant response in human monocytes via reverse signaling. Arthritis Res Ther. 2016;18:56 pubmed publisher
	Silva M, Nascimento T, Pereira M, Siqueira A, Brum P, Jaeger R, et al. Î²2-Adrenoceptor is involved in connective tissue remodeling in regenerating muscles by decreasing the activity of MMP-9. Cell Tissue Res. 2016;365:173-86 pubmed publisher

product information

Catalog Number :

ABS16

Subcategory :

Cell Signaling

Product Name :

Anti-GAPDH Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P04406

Host Name :

Rabbit

Antigen :

GAPDH

Conjugate :

Affinity Purified

Product Description :

Anti-GAPDH Antibody

Cross Reactivity :

Human;Mouse;Rat;Bovine;Guinea Pig

Background :

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in moderately high levels in almost all tissues. As a 'house-keeping' enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer's and Huntington's disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).

ALT Names :

glyceraldehyde 3-phosphate dehydrogenase;aging-associated gene 9 protein;glyceraldehyde-3-phosphate dehydrogenase

Immunogen :

GST-tagged recombinant protein corresponding to the C-terminus of human GAPDH.

Specificity :

This antibody recognizes GAPDH at the C-terminus.

Package Size :

100 μg

Uses :

Western Blotting

Storage :

Stable for 1 year at 2-8°C from date of receipt