NeuN Antibody | EMD Millipore ABN90 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

NeuN Antibody

catalog :

ABN90

quantity :

100 µL

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - free floating section

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; rat; 1:500; loading ...; fig 3g	Li J, Tao T, Xu J, Liu Z, Zou Z, Jin M. HIF‑1α attenuates neuronal apoptosis by upregulating EPO expression following cerebral ischemia‑reperfusion injury in a rat MCAO model. Int J Mol Med. 2020;45:1027-1036 pubmed publisher
immunohistochemistry; mouse; 1:2000; loading ...; fig 1c	Wu Z, Parry M, Hou X, Liu M, Wang H, Cain R, et al. Gene therapy conversion of striatal astrocytes into GABAergic neurons in mouse models of Huntington's disease. Nat Commun. 2020;11:1105 pubmed publisher
immunohistochemistry; mouse; 1:1000; loading ...; fig 1a	Barford K, Yap C, Dwyer N, Winckler B. The related neuronal endosomal proteins NEEP21 (Nsg1) and P19 (Nsg2) have divergent expression profiles in vivo. J Comp Neurol. 2017;525:1861-1878 pubmed publisher
immunohistochemistry - free floating section; rat; 1:100; fig 5	Delbary Gossart S, Lee S, Baroni M, Lamarche I, Arnone M, Canolle B, et al. A novel inhibitor of p75-neurotrophin receptor improves functional outcomes in two models of traumatic brain injury. Brain. 2016;139:1762-82 pubmed publisher
	Chen W, Cai Z, Chao E, Chen H, Longley C, Hao S, et al. Stxbp1/Munc18-1 haploinsufficiency impairs inhibition and mediates key neurological features of STXBP1 encephalopathy. elife. 2020;9: pubmed publisher
	Tian R, Gachechiladze M, Ludwig C, Laurie M, Hong J, Nathaniel D, et al. CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons. Neuron. 2019;104:239-255.e12 pubmed publisher
	Thompson S, Pitcher M, Stone L, Tarum F, Niu G, Chen X, et al. Chronic neuropathic pain reduces opioid receptor availability with associated anhedonia in rat. Pain. 2018;159:1856-1866 pubmed publisher
	Rivat C, Sar C, Mechaly I, Leyris J, Diouloufet L, Sonrier C, et al. Inhibition of neuronal FLT3 receptor tyrosine kinase alleviates peripheral neuropathic pain in mice. Nat Commun. 2018;9:1042 pubmed publisher
	Dias D, Kim H, Holl D, Werne Solnestam B, Lundeberg J, Carlen M, et al. Reducing Pericyte-Derived Scarring Promotes Recovery after Spinal Cord Injury. Cell. 2018;173:153-165.e22 pubmed publisher
	Dukkipati S, Garrett T, Elbasiouny S. The vulnerability of spinal motoneurons and soma size plasticity in a mouse model of amyotrophic lateral sclerosis. J Physiol. 2018;596:1723-1745 pubmed publisher
	Liu W, Zhao L, Blackman B, Parmar M, Wong M, Woo T, et al. Vectored Intracerebral Immunization with the Anti-Tau Monoclonal Antibody PHF1 Markedly Reduces Tau Pathology in Mutant Tau Transgenic Mice. J Neurosci. 2016;36:12425-12435 pubmed

product information

Catalog Number :

ABN90

Subcategory :

Neuroscience

Product Name :

Anti-NeuN Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Host Name :

Guinea Pig

Antigen :

NeuN

Conjugate :

Serum

Product Description :

Anti-NeuN Antibody

Cross Reactivity :

Mouse;Rat

Background :

ABN90 is a Guinea Pig polyclonal version of the Anti-NeuN, clone A60 (MAB377), a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are some examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

Immunogen :

GST-tagged recombinant protein corresponding to the N-terminus of mouse NeuN.

Specificity :

This antibody recognizes the N-terminus of NeuN.

Package Size :

100 µL

Uses :

Western Blotting;Immunohistochemistry (Paraffin);Immunocytochemistry

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.