This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
NeuN (rabbit) Antibody
catalog :
ABN78
quantity :
100 μg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat, dogs
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section
citations: 76
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:2000; loading ...; fig 1c
Wu Z, Parry M, Hou X, Liu M, Wang H, Cain R, et al. Gene therapy conversion of striatal astrocytes into GABAergic neurons in mouse models of Huntington's disease. Nat Commun. 2020;11:1105 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:300; loading ...; fig s5a
Miyawaki T, Morikawa S, Susaki E, Nakashima A, Takeuchi H, Yamaguchi S, et al. Visualization and molecular characterization of whole-brain vascular networks with capillary resolution. Nat Commun. 2020;11:1104 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:2000; loading ...; fig s1d
Ballabio C, Anderle M, Gianesello M, Lago C, Miele E, Cardano M, et al. Modeling medulloblastoma in vivo and with human cerebellar organoids. Nat Commun. 2020;11:583 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 3b
Robison L, Albert N, Camargo L, Anderson B, Salinero A, Riccio D, et al. High-Fat Diet-Induced Obesity Causes Sex-Specific Deficits in Adult Hippocampal Neurogenesis in Mice. Eneuro. 2020;7: pubmed publisher
  • immunohistochemistry; dogs; 1:1000; loading ...; fig 3h
Bekiari C, Grivas I, Tsingotjidou A, Papadopoulos G. Adult neurogenesis and gliogenesis in the dorsal and ventral canine hippocampus. J Comp Neurol. 2019;: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 3e
Zhang Q, Zhu W, Xu F, Dai X, Shi L, Cai W, et al. The interleukin-4/PPARγ signaling axis promotes oligodendrocyte differentiation and remyelination after brain injury. PLoS Biol. 2019;17:e3000330 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 1c
Saito Y, Yuan Y, Zucker Scharff I, Fak J, Jereb S, Tajima Y, et al. Differential NOVA2-Mediated Splicing in Excitatory and Inhibitory Neurons Regulates Cortical Development and Cerebellar Function. Neuron. 2019;101:707-720.e5 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; fig 4f
Segarra M, Aburto M, Cop F, Llaó Cid C, Härtl R, Damm M, et al. Endothelial Dab1 signaling orchestrates neuro-glia-vessel communication in the central nervous system. Science. 2018;361: pubmed publisher
  • immunohistochemistry; human; 1:500; loading ...; fig s21g
Sousa A, Zhu Y, Raghanti M, Kitchen R, Onorati M, Tebbenkamp A, et al. Molecular and cellular reorganization of neural circuits in the human lineage. Science. 2017;358:1027-1032 pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 1b
Zhao T, Hong Y, Yin P, Li S, Li X. Differential HspBP1 expression accounts for the greater vulnerability of neurons than astrocytes to misfolded proteins. Proc Natl Acad Sci U S A. 2017;114:E7803-E7811 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3c
  • western blot; mouse; loading ...; fig 1a
Yang Y, Yang S, Guo J, Cui Y, Tang B, Li X, et al. Synergistic Toxicity of Polyglutamine-Expanded TATA-Binding Protein in Glia and Neuronal Cells: Therapeutic Implications for Spinocerebellar Ataxia 17. J Neurosci. 2017;37:9101-9115 pubmed publisher
  • western blot; mouse; loading ...; fig s1b
Mews P, Donahue G, Drake A, Luczak V, Abel T, Berger S. Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory. Nature. 2017;546:381-386 pubmed publisher
  • immunohistochemistry; mouse; 1:750; fig 4a
Bragg R, Coffey S, Weston R, Ament S, Cantle J, Minnig S, et al. Motivational, proteostatic and transcriptional deficits precede synapse loss, gliosis and neurodegeneration in the B6.HttQ111/+ model of Huntington's disease. Sci Rep. 2017;7:41570 pubmed publisher
  • immunohistochemistry; mouse; 1:100; loading ...; fig s4e
Tufail Y, Cook D, Fourgeaud L, Powers C, Merten K, Clark C, et al. Phosphatidylserine Exposure Controls Viral Innate Immune Responses by Microglia. Neuron. 2017;93:574-586.e8 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 2c
Meng Z, Zhao T, Zhou K, Zhong Q, Wang Y, Xiong X, et al. A20 Ameliorates Intracerebral Hemorrhage-Induced Inflammatory Injury by Regulating TRAF6 Polyubiquitination. J Immunol. 2017;198:820-831 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; loading ...; tbl 1
Bassett E, Tokarew N, Allemano E, Mazerolle C, Morin K, Mears A, et al. Norrin/Frizzled4 signalling in the preneoplastic niche blocks medulloblastoma initiation. elife. 2016;5: pubmed publisher
  • immunohistochemistry - free floating section; rat; 1:1000; loading ...; tbl 2
Shepherd D, Tsai S, O Brien T, Farrer R, Kartje G. Anti-Nogo-A Immunotherapy Does Not Alter Hippocampal Neurogenesis after Stroke in Adult Rats. Front Neurosci. 2016;10:467 pubmed
  • immunohistochemistry; mouse; loading ...; fig 2h
Giannakopoulou A, Lyras G, Grigoriadis N. Long-term effects of autoimmune CNS inflammation on adult hippocampal neurogenesis. J Neurosci Res. 2017;95:1446-1458 pubmed publisher
  • western blot; mouse; loading ...
Hong Y, Zhao T, Li X, Li S. Mutant Huntingtin Impairs BDNF Release from Astrocytes by Disrupting Conversion of Rab3a-GTP into Rab3a-GDP. J Neurosci. 2016;36:8790-801 pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig 7c
Bombeiro A, Thomé R, Oliveira Nunes S, Monteiro Moreira B, Verinaud L, Oliveira A. MHC-I and PirB Upregulation in the Central and Peripheral Nervous System following Sciatic Nerve Injury. PLoS ONE. 2016;11:e0161463 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 3
Vernay A, Therreau L, Blot B, Risson V, Dirrig Grosch S, Waegaert R, et al. A transgenic mouse expressing CHMP2Bintron5 mutant in neurons develops histological and behavioural features of amyotrophic lateral sclerosis and frontotemporal dementia. Hum Mol Genet. 2016;25:3341-3360 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 4b
Anesten F, Holt M, Schéle E, Pálsdóttir V, Reimann F, Gribble F, et al. Preproglucagon neurons in the hindbrain have IL-6 receptor-α and show Ca2+ influx in response to IL-6. Am J Physiol Regul Integr Comp Physiol. 2016;311:R115-23 pubmed publisher
  • western blot; human; fig 4
Patzke C, Acuna C, Giam L, Wernig M, Südhof T. Conditional deletion of L1CAM in human neurons impairs both axonal and dendritic arborization and action potential generation. J Exp Med. 2016;213:499-515 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; fig s3a
Nagao M, Ogata T, Sawada Y, Gotoh Y. Zbtb20 promotes astrocytogenesis during neocortical development. Nat Commun. 2016;7:11102 pubmed publisher
  • immunocytochemistry; rat; 1:100; fig 1
Vincent K, Cornea V, Jong Y, Laferriere A, Kumar N, Mickeviciute A, et al. Intracellular mGluR5 plays a critical role in neuropathic pain. Nat Commun. 2016;7:10604 pubmed publisher
  • immunocytochemistry; human; fig 6
Wu H, Wang C, Liu B, Li H, Zhang Y, Dong S, et al. Altered Expression Pattern of Acid-Sensing Ion Channel Isoforms in Piriform Cortex After Seizures. Mol Neurobiol. 2016;53:1782-1793 pubmed publisher
  • western blot; mouse; 1:1000
Hung Y, Chang S, Huang C, Yin J, Hwang C, Yang L, et al. Inhibitor of Differentiation-1 and Hypoxia-Inducible Factor-1 Mediate Sonic Hedgehog Induction by Amyloid Beta-Peptide in Rat Cortical Neurons. Mol Neurobiol. 2016;53:793-809 pubmed publisher
Kaji S, Maki T, Ueda J, Ishimoto T, Inoue Y, Yasuda K, et al. BCAS1-positive immature oligodendrocytes are affected by the α-synuclein-induced pathology of multiple system atrophy. Acta Neuropathol Commun. 2020;8:120 pubmed publisher
Tsai M, Cheng H, Nian F, Liu C, Chao N, Chiang K, et al. Impairment in dynein-mediated nuclear translocation by BICD2 C-terminal truncation leads to neuronal migration defect and human brain malformation. Acta Neuropathol Commun. 2020;8:106 pubmed publisher
Leong W, Xu W, Wang B, Gao S, Zhai X, Wang C, et al. PP2A subunit PPP2R2C is downregulated in the brains of Alzheimer's transgenic mice. Aging (Albany NY). 2020;12:6880-6890 pubmed publisher
Schultz M, Fawaz M, Azaria R, Hollon T, Liu E, Kunkel T, et al. Synthetic high-density lipoprotein nanoparticles for the treatment of Niemann-Pick diseases. BMC Med. 2019;17:200 pubmed publisher
Nguyen M, Le Pichon C, RYBA N. Stereotyped transcriptomic transformation of somatosensory neurons in response to injury. elife. 2019;8: pubmed publisher
Patzke C, Brockmann M, Dai J, Gan K, Grauel M, Fenske P, et al. Neuromodulator Signaling Bidirectionally Controls Vesicle Numbers in Human Synapses. Cell. 2019;179:498-513.e22 pubmed publisher
Gao L, Zhang Z, Lu J, Pei G. Mitochondria Are Dynamically Transferring Between Human Neural Cells and Alexander Disease-Associated GFAP Mutations Impair the Astrocytic Transfer. Front Cell Neurosci. 2019;13:316 pubmed publisher
Marro S, Chanda S, Yang N, Janas J, Valperga G, Trotter J, et al. Neuroligin-4 Regulates Excitatory Synaptic Transmission in Human Neurons. Neuron. 2019;: pubmed publisher
Smith A, Duan T, Verkman A. Aquaporin-4 reduces neuropathology in a mouse model of Alzheimer's disease by remodeling peri-plaque astrocyte structure. Acta Neuropathol Commun. 2019;7:74 pubmed publisher
Chen X, Kordich J, Williams E, Levine N, Cole Strauss A, Marshall L, et al. Parkinson's disease-linked D620N VPS35 knockin mice manifest tau neuropathology and dopaminergic neurodegeneration. Proc Natl Acad Sci U S A. 2019;116:5765-5774 pubmed publisher
Yin P, Guo X, Yang W, Yan S, Yang S, Zhao T, et al. Caspase-4 mediates cytoplasmic accumulation of TDP-43 in the primate brains. Acta Neuropathol. 2019;137:919-937 pubmed publisher
Magno L, Lessard C, Martins M, Lang V, Cruz P, Asi Y, et al. Alzheimer's disease phospholipase C-gamma-2 (PLCG2) protective variant is a functional hypermorph. Alzheimers Res Ther. 2019;11:16 pubmed publisher
Jiang L, Ash P, Maziuk B, Ballance H, Boudeau S, Abdullatif A, et al. TIA1 regulates the generation and response to toxic tau oligomers. Acta Neuropathol. 2019;137:259-277 pubmed publisher
Rosenberg R, Fu M, Lambracht Washington D. Active full-length DNA Aβ42 immunization in 3xTg-AD mice reduces not only amyloid deposition but also tau pathology. Alzheimers Res Ther. 2018;10:115 pubmed publisher
Tewari B, Chaunsali L, Campbell S, Patel D, Goode A, Sontheimer H. Perineuronal nets decrease membrane capacitance of peritumoral fast spiking interneurons in a model of epilepsy. Nat Commun. 2018;9:4724 pubmed publisher
Gao Z, Davis C, Thomas A, Economo M, Abrego A, Svoboda K, et al. A cortico-cerebellar loop for motor planning. Nature. 2018;563:113-116 pubmed publisher
Geng A, Qiu R, Murai K, Liu J, Wu X, Zhang H, et al. KIF20A/MKLP2 regulates the division modes of neural progenitor cells during cortical development. Nat Commun. 2018;9:2707 pubmed publisher
Weyn Vanhentenryck S, Feng H, Ustianenko D, Duffié R, Yan Q, Jacko M, et al. Precise temporal regulation of alternative splicing during neural development. Nat Commun. 2018;9:2189 pubmed publisher
Nicholson A, Zhou X, Perkerson R, Parsons T, Chew J, Brooks M, et al. Loss of Tmem106b is unable to ameliorate frontotemporal dementia-like phenotypes in an AAV mouse model of C9ORF72-repeat induced toxicity. Acta Neuropathol Commun. 2018;6:42 pubmed publisher
Poston R, Dunn C, Sarkar P, Saha R. Persistent 6-OH-BDE-47 exposure impairs functional neuronal maturation and alters expression of neurodevelopmentally-relevant chromatin remodelers. Environ Epigenet. 2018;4:dvx020 pubmed publisher
Guy J, Alexander Howden B, Fitzpatrick L, Desousa D, Koerner M, Selfridge J, et al. A mutation-led search for novel functional domains in MeCP2. Hum Mol Genet. 2018;27:2531-2545 pubmed publisher
Wang Y, Gao Y, Tian Q, Deng Q, Wang Y, Zhou T, et al. TRPV1 SUMOylation regulates nociceptive signaling in models of inflammatory pain. Nat Commun. 2018;9:1529 pubmed publisher
Hwang M, Bergmann C. Alpha/Beta Interferon (IFN-?/?) Signaling in Astrocytes Mediates Protection against Viral Encephalomyelitis and Regulates IFN-?-Dependent Responses. J Virol. 2018;92: pubmed publisher
Beckmann N, Giorgetti E, Neuhaus A, Zurbruegg S, Accart N, Smith P, et al. Brain region-specific enhancement of remyelination and prevention of demyelination by the CSF1R kinase inhibitor BLZ945. Acta Neuropathol Commun. 2018;6:9 pubmed publisher
Lin Y, Kuo K, Chen S, Huang H. RBFOX3/NeuN is dispensable for visual function. PLoS ONE. 2018;13:e0192355 pubmed publisher
Seven Y, Perim R, Hobson O, Simon A, Tadjalli A, Mitchell G. Phrenic motor neuron adenosine 2A receptors elicit phrenic motor facilitation. J Physiol. 2018;596:1501-1512 pubmed publisher
Zhong P, Vickstrom C, Liu X, Hu Y, Yu L, Yu H, et al. HCN2 channels in the ventral tegmental area regulate behavioral responses to chronic stress. elife. 2018;7: pubmed publisher
Yi H, Liu S, Kashiwagi Y, Ikegami D, Huang W, Kanda H, et al. Phosphorylated CCAAT/Enhancer Binding Protein β Contributes to Rat HIV-Related Neuropathic Pain: In Vitro and In Vivo Studies. J Neurosci. 2018;38:555-574 pubmed publisher
Seth B, Yadav A, Agarwal S, Tiwari S, Chaturvedi R. Inhibition of the transforming growth factor-β/SMAD cascade mitigates the anti-neurogenic effects of the carbamate pesticide carbofuran. J Biol Chem. 2017;292:19423-19440 pubmed publisher
Beretta S, Cunningham K, Haus D, Gold E, Perez H, López Velázquez L, et al. Effects of Human ES-Derived Neural Stem Cell Transplantation and Kindling in a Rat Model of Traumatic Brain Injury. Cell Transplant. 2017;26:1247-1261 pubmed publisher
Lin Y, Chen C, Huang C, Nishimori K, Hsu K. Oxytocin stimulates hippocampal neurogenesis via oxytocin receptor expressed in CA3 pyramidal neurons. Nat Commun. 2017;8:537 pubmed publisher
Hong Y, Zhao T, Li X, Li S. Mutant Huntingtin Inhibits αB-Crystallin Expression and Impairs Exosome Secretion from Astrocytes. J Neurosci. 2017;37:9550-9563 pubmed publisher
Papouin T, Dunphy J, Tolman M, Dineley K, Haydon P. Septal Cholinergic Neuromodulation Tunes the Astrocyte-Dependent Gating of Hippocampal NMDA Receptors to Wakefulness. Neuron. 2017;94:840-854.e7 pubmed publisher
Mizielinska S, Ridler C, Balendra R, Thoeng A, Woodling N, Grässer F, et al. Bidirectional nucleolar dysfunction in C9orf72 frontotemporal lobar degeneration. Acta Neuropathol Commun. 2017;5:29 pubmed publisher
Long Q, Upadhya D, Hattiangady B, Kim D, An S, Shuai B, et al. Intranasal MSC-derived A1-exosomes ease inflammation, and prevent abnormal neurogenesis and memory dysfunction after status epilepticus. Proc Natl Acad Sci U S A. 2017;114:E3536-E3545 pubmed publisher
Sun W, Cornwell A, Li J, Peng S, Osorio M, Aalling N, et al. SOX9 Is an Astrocyte-Specific Nuclear Marker in the Adult Brain Outside the Neurogenic Regions. J Neurosci. 2017;37:4493-4507 pubmed publisher
Kim Y, Kim H, Hong K, Shin H, Choi B. Antidepressant Effects of Aripiprazole Augmentation for Cilostazol-Treated Mice Exposed to Chronic Mild Stress after Ischemic Stroke. Int J Mol Sci. 2017;18: pubmed publisher
Brown P, Palacorolla H, Brady D, Riegger K, Elmer G, Shepard P. Habenula-Induced Inhibition of Midbrain Dopamine Neurons Is Diminished by Lesions of the Rostromedial Tegmental Nucleus. J Neurosci. 2017;37:217-225 pubmed publisher
Zukor K, Wang H, Hurst B, Siddharthan V, van Wettere A, Pilowsky P, et al. Phrenic nerve deficits and neurological immunopathology associated with acute West Nile virus infection in mice and hamsters. J Neurovirol. 2017;23:186-204 pubmed publisher
Chen N, Sugihara H, Kim J, Fu Z, Barak B, Sur M, et al. Direct modulation of GFAP-expressing glia in the arcuate nucleus bi-directionally regulates feeding. elife. 2016;5: pubmed publisher
Chen C, Chen N, Feng C, Cheng S, Hung H, Tsui K, et al. A Coral-Derived Compound Improves Functional Recovery after Spinal Cord Injury through Its Antiapoptotic and Anti-Inflammatory Effects. Mar Drugs. 2016;14: pubmed publisher
Khodanovich M, Kisel A, Chernysheva G, Smol yakova V, Savchenko R, Plotnikov M. Effect of Fluoxetine on Neurogenesis in Hippocampal Dentate Gyrus after Global Transient Cerebral Ischemia in Rats. Bull Exp Biol Med. 2016;161:351-4 pubmed publisher
Song Z, Xiong B, Guan X, Cao F, Manyande A, Zhou Y, et al. Minocycline attenuates bone cancer pain in rats by inhibiting NF-?B in spinal astrocytes. Acta Pharmacol Sin. 2016;37:753-62 pubmed publisher
Lee K, Huang X, Fitzpatrick D. Topology of ON and OFF inputs in visual cortex enables an invariant columnar architecture. Nature. 2016;533:90-4 pubmed publisher
Carroll J, Striebel J, Rangel A, Woods T, Phillips K, Peterson K, et al. Prion Strain Differences in Accumulation of PrPSc on Neurons and Glia Are Associated with Similar Expression Profiles of Neuroinflammatory Genes: Comparison of Three Prion Strains. PLoS Pathog. 2016;12:e1005551 pubmed publisher
Williams A, White R, Siniard A, Corneveaux J, Huentelman M, Duch C. MECP2 impairs neuronal structure by regulating KIBRA. Neurobiol Dis. 2016;91:284-91 pubmed publisher
Cho J, Park J, Ahn J, Lee J, Hwang I, Park S, et al. Vanillin and 4-hydroxybenzyl alcohol promotes cell proliferation and neuroblast differentiation in the dentate gyrus of mice via the increase of brain-derived neurotrophic factor and tropomyosin-related kinase B. Mol Med Rep. 2016;13:2949-56 pubmed publisher
Carletti F, Sardo P, Gambino G, Liu X, Ferraro G, Rizzo V. Hippocampal Hyperexcitability is Modulated by Microtubule-Active Agent: Evidence from In Vivo and In Vitro Epilepsy Models in the Rat. Front Cell Neurosci. 2016;10:29 pubmed publisher
Shen S, Myers C, Hughes A, Byrne L, Flannery J, Corbo J. Massively parallel cis-regulatory analysis in the mammalian central nervous system. Genome Res. 2016;26:238-55 pubmed publisher
product information
Catalog Number :
ABN78
Subcategory :
Neuroscience
Product Name :
Anti-NeuN (rabbit) Antibody
Product Type :
Antibodies
Clonality :
Polyclonal Antibody
Host Name :
Rabbit
Antigen :
NeuN (rabbit)
Conjugate :
Affinity Purified
Product Description :
Anti-NeuN (rabbit) Antibody
Cross Reactivity :
Mouse;Human;Rat
Background :
ABN78 is a rabbit polyclonal version of the Anti-NeuN, clone A60 (MAB377), a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
ALT Names :
NEUronal Nuclei;clone A60;NeuN
Immunogen :
GST-tagged recombinant protein corresponding to mouse NeuN.
Specificity :
This antibody recognizes NeuN at the N-terminus.
Package Size :
100 μg
Uses :
Western Blotting;Immunohistochemistry (Paraffin);Immunocytochemistry
Storage :
Stable for 1 year at 2-8°C from date of receipt
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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