Histone H3.3 Antibody, K27M mutant | EMD Millipore ABE419 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Histone H3.3 Antibody, K27M mutant

catalog :

ABE419

quantity :

100 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, chromatin immunoprecipitation, immunohistochemistry - paraffin section

citations: 17

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:1000; loading ...; fig 1c	Enomoto T, Aoki M, Hamasaki M, Abe H, Nonaka M, Inoue T, et al. Midline Glioma in Adults: Clinicopathological, Genetic, and Epigenetic Analysis. Neurol Med Chir (Tokyo). 2020;60:136-146 pubmed publisher
immunohistochemistry - paraffin section; human; 1:500; loading ...; fig 1f	Abe H, Natsumeda M, Okada M, Watanabe J, Tsukamoto Y, Kanemaru Y, et al. MGMT Expression Contributes to Temozolomide Resistance in H3K27M-Mutant Diffuse Midline Gliomas. Front Oncol. 2019;9:1568 pubmed publisher
western blot; human; loading ...; fig 1a	Deng H, Zeng J, Zhang T, Gong L, Zhang H, Cheung E, et al. Histone H3.3K27M Mobilizes Multiple Cancer/Testis (CT) Antigens in Pediatric Glioma. Mol Cancer Res. 2018;16:623-633 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig 3c	Marigil M, Martinez Vélez N, Dominguez P, Idoate M, Xipell E, Patino Garcia A, et al. Development of a DIPG Orthotopic Model in Mice Using an Implantable Guide-Screw System. PLoS ONE. 2017;12:e0170501 pubmed publisher
	Chai R, Zhang Y, Liu Y, Chang Y, Pang B, Jiang T, et al. The molecular characteristics of spinal cord gliomas with or without H3 K27M mutation. Acta Neuropathol Commun. 2020;8:40 pubmed publisher
	Nagaraja S, Quezada M, Gillespie S, Arzt M, Lennon J, Woo P, et al. Histone Variant and Cell Context Determine H3K27M Reprogramming of the Enhancer Landscape and Oncogenic State. Mol Cell. 2019;76:965-980.e12 pubmed publisher
	Qi J, Esfahani D, Huang T, Ozark P, Bartom E, Hashizume R, et al. Tenascin-C expression contributes to pediatric brainstem glioma tumor phenotype and represents a novel biomarker of disease. Acta Neuropathol Commun. 2019;7:75 pubmed publisher
	Harutyunyan A, Krug B, Chen H, Papillon Cavanagh S, Zeinieh M, De Jay N, et al. H3K27M induces defective chromatin spread of PRC2-mediated repressive H3K27me2/me3 and is essential for glioma tumorigenesis. Nat Commun. 2019;10:1262 pubmed publisher
	Larson J, Kasper L, Paugh B, Jin H, Wu G, Kwon C, et al. Histone H3.3 K27M Accelerates Spontaneous Brainstem Glioma and Drives Restricted Changes in Bivalent Gene Expression. Cancer Cell. 2019;35:140-155.e7 pubmed publisher
	Liu A, Harreld J, Jacola L, Gero M, Acharya S, Ghazwani Y, et al. Tectal glioma as a distinct diagnostic entity: a comprehensive clinical, imaging, histologic and molecular analysis. Acta Neuropathol Commun. 2018;6:101 pubmed publisher
	Gits H, Anderson M, Stallard S, Pratt D, Zon B, Howell C, et al. Medulloblastoma therapy generates risk of a poorly-prognostic H3 wild-type subgroup of diffuse intrinsic pontine glioma: a report from the International DIPG Registry. Acta Neuropathol Commun. 2018;6:67 pubmed publisher
	Stone T, Keeley A, Virasami A, Harkness W, Tisdall M, Izquierdo Delgado E, et al. Comprehensive molecular characterisation of epilepsy-associated glioneuronal tumours. Acta Neuropathol. 2018;135:115-129 pubmed publisher
	Nomura M, Mukasa A, Nagae G, Yamamoto S, Tatsuno K, Ueda H, et al. Distinct molecular profile of diffuse cerebellar gliomas. Acta Neuropathol. 2017;134:941-956 pubmed publisher
	Haque F, Varlet P, Puntonet J, Storer L, Bountali A, Rahman R, et al. Evaluation of a novel antibody to define histone 3.3 G34R mutant brain tumours. Acta Neuropathol Commun. 2017;5:45 pubmed publisher
	Huang T, Piunti A, Lulla R, Qi J, Horbinski C, Tomita T, et al. Detection of Histone H3 mutations in cerebrospinal fluid-derived tumor DNA from children with diffuse midline glioma. Acta Neuropathol Commun. 2017;5:28 pubmed publisher
	Piunti A, Hashizume R, Morgan M, Bartom E, Horbinski C, Marshall S, et al. Therapeutic targeting of polycomb and BET bromodomain proteins in diffuse intrinsic pontine gliomas. Nat Med. 2017;23:493-500 pubmed publisher
	Nikbakht H, Panditharatna E, Mikael L, Li R, Gayden T, Osmond M, et al. Spatial and temporal homogeneity of driver mutations in diffuse intrinsic pontine glioma. Nat Commun. 2016;7:11185 pubmed publisher

product information

Catalog Number :

ABE419

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Histone H3.3 Antibody, K27M mutant

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P84243

Host Name :

Rabbit

Antigen :

Histone H3.3

Conjugate :

Affinity Purified

Product Description :

Anti-Histone H3.3 Antibody, K27M mutant

Cross Reactivity :

Mouse;Human

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone variant H3.3 is typically enriched in active chromatin.

ALT Names :

Histone H3.3

Immunogen :

KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H3.3, K27M mutant.

Specificity :

This antibody recognizes the N-terminus of Histone H3.3, K27M mutant.

Package Size :

100 μg

Uses :

Western Blotting;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at 2-8°C from date of receipt