NMDAR1 Antibody, rabbit monoclonal | EMD Millipore AB9864 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

NMDAR1 Antibody, rabbit monoclonal

catalog :

AB9864

quantity :

50 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

1.17.2.6

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, blocking or activating experiments

citations: 39

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; loading ...; fig s1i	Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, et al. Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting. Science. 2019;363: pubmed publisher
immunocytochemistry; human; 1:1000; loading ...; fig s4a	Tseng J, Xie L, Song S, Xie Y, Allen L, Ajit D, et al. The Deacetylase HDAC6 Mediates Endogenous Neuritic Tau Pathology. Cell Rep. 2017;20:2169-2183 pubmed publisher
immunoprecipitation; rat; 1:1000; loading ...; fig 5d immunohistochemistry; rat; 1:1000; loading ...; fig 4g western blot; rat; 1:1000; loading ...; fig 3a	Kim J, Ko A, Hyun H, Min S, Kim J. PDI regulates seizure activity via NMDA receptor redox in rats. Sci Rep. 2017;7:42491 pubmed publisher
western blot; rat; 1:2000; fig 6a	McQuail J, Beas B, Kelly K, Simpson K, Frazier C, Setlow B, et al. NR2A-Containing NMDARs in the Prefrontal Cortex Are Required for Working Memory and Associated with Age-Related Cognitive Decline. J Neurosci. 2016;36:12537-12548 pubmed
western blot; mouse; 1:500	Frank R, Komiyama N, Ryan T, Zhu F, O Dell T, Grant S. NMDA receptors are selectively partitioned into complexes and supercomplexes during synapse maturation. Nat Commun. 2016;7:11264 pubmed publisher
western blot; mouse; 1:500; fig 3	Blanco E, Galeano P, Palomino A, PavÃ³n F, Rivera P, Serrano A, et al. Cocaine-induced behavioral sensitization decreases the expression of endocannabinoid signaling-related proteins in the mouse hippocampus. Eur Neuropsychopharmacol. 2016;26:477-92 pubmed publisher
blocking or activating experiments; rat; fig 1	WÃ¼rdemann T, Kersten M, Tokay T, Guli X, Kober M, Rohde M, et al. Stereotactic injection of cerebrospinal fluid from anti-NMDA receptor encephalitis into rat dentate gyrus impairs NMDA receptor function. Brain Res. 2016;1633:10-18 pubmed publisher
western blot; mouse; 1:500; loading ...; fig 6a	Valenza M, Chen J, Di Paolo E, Ruozi B, Belletti D, Ferrari Bardile C, et al. Cholesterol-loaded nanoparticles ameliorate synaptic and cognitive function in Huntington's disease mice. EMBO Mol Med. 2015;7:1547-64 pubmed publisher
western blot; mouse	Kundu S, Pushpakumar S, Sen U. MMP-9- and NMDA receptor-mediated mechanism of diabetic renovascular remodeling and kidney dysfunction: hydrogen sulfide is a key modulator. Nitric Oxide. 2015;46:172-85 pubmed publisher
western blot; mouse; fig 6	RodrÃguez MuÃ±oz M, SÃ¡nchez BlÃ¡zquez P, Herrero Labrador R, MartÃnez Murillo R, Merlos M, Vela J, et al. The Ïƒ1 receptor engages the redox-regulated HINT1 protein to bring opioid analgesia under NMDA receptor negative control. Antioxid Redox Signal. 2015;22:799-818 pubmed publisher
immunocytochemistry; rat; 1:1000 western blot; rat; 1:1000	Peng X, Hughes E, Moscato E, Parsons T, Dalmau J, Balice Gordon R. Cellular plasticity induced by anti-Î±-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor encephalitis antibodies. Ann Neurol. 2015;77:381-98 pubmed publisher
western blot; rat; 1:1000	Savignac H, Corona G, Mills H, Chen L, Spencer J, Tzortzis G, et al. Prebiotic feeding elevates central brain derived neurotrophic factor, N-methyl-D-aspartate receptor subunits and D-serine. Neurochem Int. 2013;63:756-64 pubmed publisher
western blot; mouse; 1:1000; fig 7	Blanco E, Bilbao A, Luque Rojas M, Palomino A, Bermudez Silva F, Suarez J, et al. Attenuation of cocaine-induced conditioned locomotion is associated with altered expression of hippocampal glutamate receptors in mice lacking LPA1 receptors. Psychopharmacology (Berl). 2012;220:27-42 pubmed publisher
	Sequerra E, Goyal R, Castro P, Levin J, Borodinsky L. NMDA Receptor Signaling Is Important for Neural Tube Formation and for Preventing Antiepileptic Drug-Induced Neural Tube Defects. J Neurosci. 2018;38:4762-4773 pubmed publisher
	Calvo Rodríguez M, de la Fuente C, García Durillo M, Garcia Rodriguez C, Villalobos C, Núñez L. Aging and amyloid ? oligomers enhance TLR4 expression, LPS-induced Ca2+ responses, and neuron cell death in cultured rat hippocampal neurons. J Neuroinflammation. 2017;14:24 pubmed publisher
	Bonini D, Mora C, Tornese P, Sala N, Filippini A, La Via L, et al. Acute Footshock Stress Induces Time-Dependent Modifications of AMPA/NMDA Protein Expression and AMPA Phosphorylation. Neural Plast. 2016;2016:7267865 pubmed publisher
	Savignac H, Couch Y, Stratford M, Bannerman D, Tzortzis G, Anthony D, et al. Prebiotic administration normalizes lipopolysaccharide (LPS)-induced anxiety and cortical 5-HT2A receptor and IL1-Î² levels in male mice. Brain Behav Immun. 2016;52:120-131 pubmed publisher
	Mancuso R, del Valle J, Morell M, Pallas M, Osta R, Navarro X. Lack of synergistic effect of resveratrol and sigma-1 receptor agonist (PRE-084) in SOD1G?³A ALS mice: overlapping effects or limited therapeutic opportunity?. Orphanet J Rare Dis. 2014;9:78 pubmed publisher
	Ruocco L, Treno C, Gironi Carnevale U, Arra C, Mattern C, Huston J, et al. Prepuberal intranasal dopamine treatment in an animal model of ADHD ameliorates deficient spatial attention, working memory, amino acid transmitters and synaptic markers in prefrontal cortex, ventral and dorsal striatum. Amino Acids. 2014;46:2105-22 pubmed publisher
	Nikitczuk J, Patil S, Matikainen Ankney B, Scarpa J, Shapiro M, Benson D, et al. N-cadherin regulates molecular organization of excitatory and inhibitory synaptic circuits in adult hippocampus in vivo. Hippocampus. 2014;24:943-962 pubmed publisher
	Ruocco L, Treno C, Gironi Carnevale U, Arra C, Boatto G, Nieddu M, et al. Prepuberal stimulation of 5-HT7-R by LP-211 in a rat model of hyper-activity and attention-deficit: permanent effects on attention, brain amino acids and synaptic markers in the fronto-striatal interface. PLoS ONE. 2014;9:e83003 pubmed publisher
	Kung L, Gong K, Adedoyin M, Ng J, Bhargava A, Ohara P, et al. Evidence for glutamate as a neuroglial transmitter within sensory ganglia. PLoS ONE. 2013;8:e68312 pubmed publisher
	Kamada T, Sun W, Takase K, Shigeto H, Suzuki S, Ohyagi Y, et al. Spontaneous seizures in a rat model of multiple prenatal freeze lesioning. Epilepsy Res. 2013;105:280-91 pubmed publisher
	Qu X, Xu C, Wang H, Xu J, Liu W, Wang Y, et al. Hippocampal glutamate level and glutamate aspartate transporter (GLAST) are up-regulated in senior rat associated with isoflurane-induced spatial learning/memory impairment. Neurochem Res. 2013;38:59-73 pubmed publisher
	Gleichman A, Spruce L, Dalmau J, Seeholzer S, Lynch D. Anti-NMDA receptor encephalitis antibody binding is dependent on amino acid identity of a small region within the GluN1 amino terminal domain. J Neurosci. 2012;32:11082-94 pubmed publisher
	Di Maio R, Mastroberardino P, Hu X, Montero L, Greenamyre J. Thiol oxidation and altered NR2B/NMDA receptor functions in in vitro and in vivo pilocarpine models: implications for epileptogenesis. Neurobiol Dis. 2013;49:87-98 pubmed publisher
	Bull N, Chidlow G, Wood J, Martin K, Casson R. The mechanism of axonal degeneration after perikaryal excitotoxic injury to the retina. Exp Neurol. 2012;236:34-45 pubmed publisher
	Sproul A, Steele S, Thai T, Yu S, Klein J, Sands J, et al. N-methyl-D-aspartate receptor subunit NR3a expression and function in principal cells of the collecting duct. Am J Physiol Renal Physiol. 2011;301:F44-54 pubmed publisher
	Di Maio R, Mastroberardino P, Hu X, Montero L, Greenamyre J. Pilocapine alters NMDA receptor expression and function in hippocampal neurons: NADPH oxidase and ERK1/2 mechanisms. Neurobiol Dis. 2011;42:482-95 pubmed publisher
	Lee J, Pradhan J, Maskey D, Park K, Hong S, Suh M, et al. Glutamate co-transmission from developing medial nucleus of the trapezoid body--lateral superior olive synapses is cochlear dependent in kanamycin-treated rats. Biochem Biophys Res Commun. 2011;405:162-7 pubmed publisher
	Tirapelli D, Carlotti C, Leite J, Tirapelli L, Colli B. Expression of HSP70 in cerebral ischemia and neuroprotetive action of hypothermia and ketoprofen. Arq Neuropsiquiatr. 2010;68:592-6 pubmed
	Marignier R, Nicolle A, Watrin C, Touret M, Cavagna S, Varrin Doyer M, et al. Oligodendrocytes are damaged by neuromyelitis optica immunoglobulin G via astrocyte injury. Brain. 2010;133:2578-91 pubmed publisher
	Swanwick C, Shapiro M, Vicini S, Wenthold R. Flotillin-1 promotes formation of glutamatergic synapses in hippocampal neurons. Dev Neurobiol. 2010;70:875-83 pubmed publisher
	Fernández Lamo I, Montero Pedrazuela A, Delgado García J, Guadaño Ferraz A, Gruart A. Effects of thyroid hormone replacement on associative learning and hippocampal synaptic plasticity in adult hypothyroid rats. Eur J Neurosci. 2009;30:679-92 pubmed publisher
	Florance N, Davis R, Lam C, Szperka C, Zhou L, Ahmad S, et al. Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis in children and adolescents. Ann Neurol. 2009;66:11-8 pubmed publisher
	Liu J, Yao Y, Jiang W. Inhibitory effects of Group I metabotropic glutamate receptors antagonists on the expression of NMDA receptor NR1 subunit in morphine tolerant rats. Neurosci Lett. 2009;452:268-72 pubmed publisher
	Hong S, Kim M, Ahn S. Glutamatergic transmission is sustained at a later period of development of medial nucleus of the trapezoid body-lateral superior olive synapses in circling mice. J Neurosci. 2008;28:13003-7 pubmed publisher
	Dalmau J, Gleichman A, Hughes E, Rossi J, Peng X, Lai M, et al. Anti-NMDA-receptor encephalitis: case series and analysis of the effects of antibodies. Lancet Neurol. 2008;7:1091-8 pubmed publisher
	Willcockson H, Valtschanoff J. AMPA and NMDA glutamate receptors are found in both peptidergic and non-peptidergic primary afferent neurons in the rat. Cell Tissue Res. 2008;334:17-23 pubmed publisher

product information

Catalog Number :

AB9864

Subcategory :

Neuroscience

Product Name :

Anti-NMDAR1 Antibody, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q05586

Host Name :

Rabbit

Antigen :

NMDAR1

Clone :

1.17.2.6

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-NMDAR1 Antibody, rabbit monoclonal

Cross Reactivity :

Rat

Background :

N-methyl-D-aspartate Receptors (NMDAR) are one of three pharmacologically distinct subtypes of ionotropic receptors that mediate a majority of excitatory neurotransmission in the brain via the endogenous amino acid, L-glutamate. MDARs form heteromeric complexes usually composed of two components, the NR1 subunit and NR2 subunits. Both proteins are weakly expressed throughout the brain but show localization in the dendritic region of the mushroom bodies. The NMDAR channel is highly permeable to Ca2+ and Na+ and its opening requires the simultaneous binding of glutamate and postsynaptic membrane depolarization. The activated NMDAR channel allows calcium influx into the post-synaptic cell where calcium triggers a cascade of biochemical events that create synaptic changes. NMDAR have been studied extensively in Drosophila for its potential relationship to behavioral plasticity. A newly identified NMDAR complex of more than 80 different proteins has been identified. Genetic and pharmacological disruptions of components of the NMDAR complex produce learning impairments in rodents, further linking behavioral plasticity to synaptic plasticity.

ALT Names :

N-methyl-D-aspartate receptor channel, subunit zeta-1;N-methyl-D-aspartate receptor subunit NR1;NMDA receptor 1;glutamate [NMDA] receptor subunit zeta 1;glutamate receptor, ionotropic, N-methyl D-aspartate 1

Immunogen :

Synthetic peptide corresponding to the C-terminus of rat NMDA receptor subunit (Catalog number AG344). {amino acids 909-938 rat NMDAR1{LQNQKDTVLPRRAIERE EGQLQLCSRHRES}

Specificity :

The original sequence used is selective for splice variants NR1-1a, NR1-1b, NR1-2a, NR1-2b using the nomenclature defined by Hollmann, M. et al. (1993). These appear to be the major splice variants expressed in rat brain (Sugihara H, et al., 1992). No cross-reaction with other glutamate receptor subunits. Western Blot analysis shows that this rabbit monoclonal antibody labels a single band at approximately 120 kD corresponding to NMDAR1 in rat brain lysate.

Package Size :

50 µL

Uses :

Immunohistochemistry;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt.