8-Hydroxydeoxyguanosine Antibody | EMD Millipore AB5830 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

8-Hydroxydeoxyguanosine Antibody

catalog :

AB5830

quantity :

200 μL

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse

application :

immunohistochemistry, immunohistochemistry - paraffin section

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 3a	Barca E, Kleiner G, Tang G, Ziosi M, Tadesse S, Masliah E, et al. Decreased Coenzyme Q10 Levels in Multiple System Atrophy Cerebellum. J Neuropathol Exp Neurol. 2016;75:663-72 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:1000; fig 3	Sun J, Shen R, Schrock M, Liu J, Pan X, Quimby D, et al. Reduction in squamous cell carcinomas in mouse skin by dietary zinc supplementation. Cancer Med. 2016;5:2032-42 pubmed publisher
	Amente S, Di Palo G, Scala G, Castrignanò T, Gorini F, Cocozza S, et al. Genome-wide mapping of 8-oxo-7,8-dihydro-2'-deoxyguanosine reveals accumulation of oxidatively-generated damage at DNA replication origins within transcribed long genes of mammalian cells. Nucleic Acids Res. 2019;47:221-236 pubmed publisher

product information

Catalog Number :

AB5830

Subcategory :

Neuroscience

Product Name :

Anti-8-Hydroxydeoxyguanosine Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Host Name :

Goat

Antigen :

8-Hydroxydeoxyguanosine

Conjugate :

Serum

Product Description :

Anti-8-Hydroxydeoxyguanosine Antibody

Cross Reactivity :

Human

Background :

8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer's disease. 8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress. Industrial uses may extend to the dietary supplement manufacturers, who could benefit from an immunoassay that could be used to test the effectiveness of antioxidants and other nutraceuticals. CHEMICON's new goat antiserum to 8-hydroxyguanosine is now available. This antiserum has been shown by ELISA to be completely cross-reactive with 8-hydroxydeoxyguanosine while not cross-reacting with other naturally occurring nucleotides. This antibody should be a valuable tool for scientists working to understand the role of free radical damage in a number of human disease states.

ALT Names :

8-OHdG; 8-hydroxy-2'-deoxyguanosine

Immunogen :

8-Hydroxy-2'-deoxyguanosine and 8-hydroxyguanosine.

Specificity :

Reacts with 8-Hydroxydeoxyguanosine (8-OHdG) and 8-Hydroxyguanosine (8-OHG). By a competitive ELISA the antibody was 100% inhibited by 8-hydroxydeoxyguanosine and 8-hydroxyguanosine nucleotides. Partial cross-reactivity was observed with 8-mercaptoguanosine (80%) while 8-bromoguanosine showed some cross-reactivity (5%). 2-Deoxyadenosine, 7-methlyguanosine, guanosine monophosphate and guanosine showed no reactivity.

Package Size :

200 μL

Uses :

ELISA;Immunohistochemistry (Paraffin)

Storage :

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.