Dlx2 Antibody | EMD Millipore AB5726 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Dlx2 Antibody

catalog :

AB5726

quantity :

100 µg

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry

citations: 5

Reference
Ponce Regalado M, Ortuño Sahagún D, Zarate C, Gudiño Cabrera G. Ensheathing cell-conditioned medium directs the differentiation of human umbilical cord blood cells into aldynoglial phenotype cells. Hum Cell. 2012;25:51-60 pubmed publisher
Yilmaz M, Maass D, Tiwari N, Waldmeier L, Schmidt P, Lehembre F, et al. Transcription factor Dlx2 protects from TGF?-induced cell-cycle arrest and apoptosis. EMBO J. 2011;30:4489-99 pubmed publisher
Wang Z, Li X, Li Z, Yang L, Sasaki Y, Wang S, et al. Effects of inorganic polyphosphate on bone sialoprotein gene expression. Gene. 2010;452:79-86 pubmed publisher
He P, Shen Y. Interruption of beta-catenin signaling reduces neurogenesis in Alzheimer's disease. J Neurosci. 2009;29:6545-57 pubmed publisher
Chaichana K, Guerrero Cazares H, Capilla Gonzalez V, Zamora Berridi G, Achanta P, Gonzalez Perez O, et al. Intra-operatively obtained human tissue: protocols and techniques for the study of neural stem cells. J Neurosci Methods. 2009;180:116-25 pubmed publisher

product information

Catalog Number :

AB5726

Subcategory :

Neuroscience

Product Name :

Anti-Dlx2 Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q07687

Host Name :

Rabbit

Antigen :

Dlx2

Conjugate :

Affinity Purified

Product Description :

Anti-Dlx2 Antibody

Cross Reactivity :

Human;Mouse

ALT Names :

Distalless

Immunogen :

Synthetic peptide, amino acids 24-39 from human Dlx 2.

Specificity :

Recognizes Dlx 2 (Distalless (DII) family), a homeodomain transcription factor.

Package Size :

100 µg

Uses :

Western Blotting;Immunohistochemistry

Storage :

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses. The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.