This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Sox2 Antibody
catalog :
AB5603
quantity :
100 μg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat, chicken, pigs
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 70
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; human; 1:200; loading ...; fig s4c
Wang H, Wang M, Wen Y, Xu C, Chen X, Wu D, et al. Biphasic Regulation of Mesenchymal Genes Controls Fate Switches During Hematopoietic Differentiation of Human Pluripotent Stem Cells. Adv Sci (Weinh). 2020;7:2001019 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig 1d
Marin Navarro A, Pronk R, van der Geest A, Oliynyk G, Nordgren A, Arsenian Henriksson M, et al. p53 controls genomic stability and temporal differentiation of human neural stem cells and affects neural organization in human brain organoids. Cell Death Dis. 2020;11:52 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s6d
Low J, Li P, Chew E, Zhou B, Suzuki K, Zhang T, et al. Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network. Cell Stem Cell. 2019;25:373-387.e9 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 3d
Prajapati DiNubila M, Benito Gonzalez A, Golden E, Zhang S, Doetzlhofer A. A counter gradient of Activin A and follistatin instructs the timing of hair cell differentiation in the murine cochlea. elife. 2019;8: pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 2a
Nieto González J, Gómez Sánchez L, Mavillard F, Linares Clemente P, Rivero M, Valenzuela Villatoro M, et al. Loss of postnatal quiescence of neural stem cells through mTOR activation upon genetic removal of cysteine string protein-α. Proc Natl Acad Sci U S A. 2019;116:8000-8009 pubmed publisher
  • western blot; mouse; loading ...; fig 1f
Zhong X, Cui P, Cai Y, Wang L, He X, Long P, et al. Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells. Cell Metab. 2019;29:979-992.e4 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 2h
Tahara N, Akiyama R, Theisen J, Kawakami H, Wong J, Garry D, et al. Gata6 restricts Isl1 to the posterior of nascent hindlimb buds through Isl1 cis-regulatory modules. Dev Biol. 2018;434:74-83 pubmed publisher
  • western blot; human; fig 4e
Arrizabalaga O, Moreno Cugnon L, Auzmendi Iriarte J, Aldaz P, Ibanez de Caceres I, Garros Regulez L, et al. High expression of MKP1/DUSP1 counteracts glioma stem cell activity and mediates HDAC inhibitor response. Oncogenesis. 2017;6:401 pubmed publisher
  • immunohistochemistry; mouse; 1:500; loading ...; fig s2i
Remez L, Onishi A, Menuchin Lasowski Y, Biran A, Blackshaw S, Wahlin K, et al. Pax6 is essential for the generation of late-born retinal neurons and for inhibition of photoreceptor-fate during late stages of retinogenesis. Dev Biol. 2017;432:140-150 pubmed publisher
  • immunohistochemistry - frozen section; rat; loading ...; fig 3f
Roberts S, Dun X, Doddrell R, Mindos T, Drake L, Onaitis M, et al. Sox2 expression in Schwann cells inhibits myelination in vivo and induces influx of macrophages to the nerve. Development. 2017;144:3114-3125 pubmed publisher
  • immunocytochemistry; human; 1:200; loading ...; fig s1a
Jeziorowska D, Fontaine V, Jouve C, Villard E, Dussaud S, Akbar D, et al. Differential Sarcomere and Electrophysiological Maturation of Human iPSC-Derived Cardiac Myocytes in Monolayer vs. Aggregation-Based Differentiation Protocols. Int J Mol Sci. 2017;18: pubmed publisher
  • immunohistochemistry; human; 1:3200; loading ...; fig 1
Hashimoto I, Nagata T, Sekine S, Moriyama M, Shibuya K, Hojo S, et al. Prognostic significance of KLF4 expression in gastric cancer. Oncol Lett. 2017;13:819-826 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 2c
Lo Nigro A, de Jaime Soguero A, Khoueiry R, Cho D, Ferlazzo G, Perini I, et al. PDGFR?+ Cells in Embryonic Stem Cell Cultures Represent the In Vitro Equivalent of the Pre-implantation Primitive Endoderm Precursors. Stem Cell Reports. 2017;8:318-333 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 2d
Hussain R, Macklin W. Integrin-Linked Kinase (ILK) Deletion Disrupts Oligodendrocyte Development by Altering Cell Cycle. J Neurosci. 2017;37:397-412 pubmed publisher
  • immunocytochemistry; human; 1:1000; fig s1b
Gong L, Pan X, Chen H, Rao L, Zeng Y, Hang H, et al. p53 isoform Δ133p53 promotes efficiency of induced pluripotent stem cells and ensures genomic integrity during reprogramming. Sci Rep. 2016;6:37281 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:400; loading ...; fig 8c
Bosch P, Fuller L, Sleeth C, Weiner J. Akirin2 is essential for the formation of the cerebral cortex. Neural Dev. 2016;11:21 pubmed
  • immunocytochemistry; mouse; 1:1000; fig 1f
Cortes D, Robledo Arratia Y, Hernández Martinez R, Escobedo Ávila I, Bargas J, Velasco I. Transgenic GDNF Positively Influences Proliferation, Differentiation, Maturation and Survival of Motor Neurons Produced from Mouse Embryonic Stem Cells. Front Cell Neurosci. 2016;10:217 pubmed publisher
  • immunohistochemistry - frozen section; chicken; 1:400; fig 2
Peretz Y, Eren N, Kohl A, Hen G, Yaniv K, Weisinger K, et al. A new role of hindbrain boundaries as pools of neural stem/progenitor cells regulated by Sox2. BMC Biol. 2016;14:57 pubmed publisher
  • western blot; human; fig 4
Bie Q, Sun C, Gong A, Li C, Su Z, Zheng D, et al. Non-tumor tissue derived interleukin-17B activates IL-17RB/AKT/β-catenin pathway to enhance the stemness of gastric cancer. Sci Rep. 2016;6:25447 pubmed publisher
  • immunohistochemistry; mouse; 1:250; loading ...; fig 3b
Kam J, Dumontier E, Baim C, Brignall A, Mendes da Silva D, Cowan M, et al. RGMB and neogenin control cell differentiation in the developing olfactory epithelium. Development. 2016;143:1534-46 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:1200; fig 7
Alshammari M, Alshammari T, Laezza F. Improved Methods for Fluorescence Microscopy Detection of Macromolecules at the Axon Initial Segment. Front Cell Neurosci. 2016;10:5 pubmed publisher
  • immunocytochemistry; human; 1:500; fig 2
Sancho Martinez I, Nivet E, Xia Y, Hishida T, Aguirre A, Ocampo A, et al. Establishment of human iPSC-based models for the study and targeting of glioma initiating cells. Nat Commun. 2016;7:10743 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Walter M, Teissandier A, Pérez Palacios R, Bourc his D. An epigenetic switch ensures transposon repression upon dynamic loss of DNA methylation in embryonic stem cells. elife. 2016;5: pubmed publisher
  • immunohistochemistry; mouse; 1:1000; fig 1
Grego Bessa J, Bloomekatz J, Castel P, Omelchenko T, Baselga J, Anderson K. The tumor suppressor PTEN and the PDK1 kinase regulate formation of the columnar neural epithelium. elife. 2016;5:e12034 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:100; fig 1
Misuraca K, Hu G, Barton K, Chung A, Becher O. A Novel Mouse Model of Diffuse Intrinsic Pontine Glioma Initiated in Pax3-Expressing Cells. Neoplasia. 2016;18:60-70 pubmed publisher
  • immunohistochemistry; mouse; 5 mg/ml; loading ...; fig 9c
Wymeersch F, Huang Y, Blin G, Cambray N, Wilkie R, Wong F, et al. Position-dependent plasticity of distinct progenitor types in the primitive streak. elife. 2016;5:e10042 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:100; fig 5
Benedykcinska A, Ferreira A, Lau J, Broni J, Richard Loendt A, Henriquez N, et al. Generation of brain tumours in mice by Cre-mediated recombination of neural progenitors in situ with the tamoxifen metabolite endoxifen. Dis Model Mech. 2016;9:211-20 pubmed publisher
  • immunocytochemistry; mouse; 1:200
Mallol A, Piqué L, Santaló J, Ibáñez E. Morphokinetics of cloned mouse embryos treated with epigenetic drugs and blastocyst prediction. Reproduction. 2016;151:203-14 pubmed publisher
  • immunocytochemistry; pigs ; 1:200; fig 5
Kim E, Hwang S, Yoo H, Yoon J, Jeon Y, Kim H, et al. Putative embryonic stem cells derived from porcine cloned blastocysts using induced pluripotent stem cells as donors. Theriogenology. 2016;85:601-16 pubmed publisher
  • western blot; mouse; fig 2b
Jiang Y, Du M, Wu M, Zhu Y, Zhao X, Cao X, et al. Phosphatidic Acid Improves Reprogramming to Pluripotency by Reducing Apoptosis. Stem Cells Dev. 2016;25:43-54 pubmed publisher
  • immunohistochemistry - frozen section; human; 1:400; loading ...; tbl 1
Endaya B, Cavanagh B, Alowaidi F, Walker T, de Pennington N, Ng J, et al. Isolating dividing neural and brain tumour cells for gene expression profiling. J Neurosci Methods. 2016;257:121-33 pubmed publisher
Bunina D, Abazova N, Diaz N, Noh K, Krijgsveld J, Zaugg J. Genomic Rewiring of SOX2 Chromatin Interaction Network during Differentiation of ESCs to Postmitotic Neurons. Cell Syst. 2020;10:480-494.e8 pubmed publisher
Varderidou Minasian S, Hinz L, Hagemans D, Posthuma D, Altelaar M, Heine V. Quantitative proteomic analysis of Rett iPSC-derived neuronal progenitors. Mol Autism. 2020;11:38 pubmed publisher
An Z, Liu P, Zheng J, Si C, Li T, Chen Y, et al. Sox2 and Klf4 as the Functional Core in Pluripotency Induction without Exogenous Oct4. Cell Rep. 2019;29:1986-2000.e8 pubmed publisher
Jang J, Han D, Golkaram M, Audouard M, Liu G, Bridges D, et al. Control over single-cell distribution of G1 lengths by WNT governs pluripotency. PLoS Biol. 2019;17:e3000453 pubmed publisher
Latour Y, Yoon R, Thomas S, Grant C, Li C, Sena Esteves M, et al. Human GLB1 knockout cerebral organoids: A model system for testing AAV9-mediated GLB1 gene therapy for reducing GM1 ganglioside storage in GM1 gangliosidosis. Mol Genet Metab Rep. 2019;21:100513 pubmed publisher
Song Z, Laureano A, Patel K, Yip S, Jadali A, Kwan K. Single-Cell Fluorescence Analysis of Pseudotemporal Ordered Cells Provides Protein Expression Dynamics for Neuronal Differentiation. Front Cell Dev Biol. 2019;7:87 pubmed publisher
Pereira D, Gomes S, Borralho P, Rodrigues C. MEK5/ERK5 activation regulates colon cancer stem-like cell properties. Cell Death Discov. 2019;5:68 pubmed publisher
Meyer K, Feldman H, Lu T, Drake D, Lim E, Ling K, et al. REST and Neural Gene Network Dysregulation in iPSC Models of Alzheimer's Disease. Cell Rep. 2019;26:1112-1127.e9 pubmed publisher
Coronel R, Lachgar M, Bernabeu Zornoza A, Palmer C, Domínguez Alvaro M, Revilla A, et al. Neuronal and Glial Differentiation of Human Neural Stem Cells Is Regulated by Amyloid Precursor Protein (APP) Levels. Mol Neurobiol. 2019;56:1248-1261 pubmed publisher
Méndez Maldonado K, Vega Lopez G, Caballero Chacón S, Aybar M, Velasco I. Activation of Hes1 and Msx1 in Transgenic Mouse Embryonic Stem Cells Increases Differentiation into Neural Crest Derivatives. Int J Mol Sci. 2018;19: pubmed publisher
Kim E, Hwang S, Yoon J, Kim H, Lee G, Hyun S. Isolation and characterization of GFAP-positive porcine neural stem/progenitor cells derived from a GFAP-CreERT2 transgenic piglet. BMC Vet Res. 2018;14:331 pubmed publisher
Medina Cano D, Ucuncu E, Nguyen L, Nicouleau M, Lipecka J, Bizot J, et al. High N-glycan multiplicity is critical for neuronal adhesion and sensitizes the developing cerebellum to N-glycosylation defect. elife. 2018;7: pubmed publisher
Rodrigo Albors A, Halley P, Storey K. Lineage tracing of axial progenitors using Nkx1-2CreERT2 mice defines their trunk and tail contributions. Development. 2018;145: pubmed publisher
Liu J, Matarin M, REEVES C, McEvoy A, Miserocchi A, Thompson P, et al. Doublecortin-expressing cell types in temporal lobe epilepsy. Acta Neuropathol Commun. 2018;6:60 pubmed publisher
Senís E, Mosteiro L, Wilkening S, Wiedtke E, Nowrouzi A, Afzal S, et al. AAVvector-mediated in vivo reprogramming into pluripotency. Nat Commun. 2018;9:2651 pubmed publisher
Schultz L, Haltom J, Almeida M, Wierson W, Solin S, Weiss T, et al. Epigenetic regulators Rbbp4 and Hdac1 are overexpressed in a zebrafish model of RB1 embryonal brain tumor, and are required for neural progenitor survival and proliferation. Dis Model Mech. 2018;11: pubmed publisher
Verrier L, Davidson L, Gierlinski M, Dady A, Storey K. Neural differentiation, selection and transcriptomic profiling of human neuromesodermal progenitor-like cells in vitro. Development. 2018;145: pubmed publisher
Biehl M, Kaylan K, Thompson R, Gonzalez R, Weis K, Underhill G, et al. Cellular fate decisions in the developing female anteroventral periventricular nucleus are regulated by canonical Notch signaling. Dev Biol. 2018;442:87-100 pubmed publisher
Li N, Zhang Y, Sidlauskas K, Ellis M, Evans I, Frankel P, et al. Inhibition of GPR158 by microRNA-449a suppresses neural lineage of glioma stem/progenitor cells and correlates with higher glioma grades. Oncogene. 2018;37:4313-4333 pubmed publisher
Akiyama T, Sato S, Chikazawa Nohtomi N, Soma A, Kimura H, Wakabayashi S, et al. Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing. Sci Rep. 2018;8:1189 pubmed publisher
Wang H, Zhang K, Liu Y, Fu Y, Gao S, Gong P, et al. Telomere heterogeneity linked to metabolism and pluripotency state revealed by simultaneous analysis of telomere length and RNA-seq in the same human embryonic stem cell. BMC Biol. 2017;15:114 pubmed publisher
Urbanska M, Winzi M, Neumann K, Abuhattum S, Rosendahl P, Müller P, et al. Single-cell mechanical phenotype is an intrinsic marker of reprogramming and differentiation along the mouse neural lineage. Development. 2017;144:4313-4321 pubmed publisher
Pontes Quero S, Heredia L, Casquero García V, Fernández Chacón M, Luo W, Hermoso A, et al. Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis. Cell. 2017;170:800-814.e18 pubmed publisher
Zhang Y, Kim M, Jia B, Yan J, Zuniga Hertz J, Han C, et al. Hypothalamic stem cells control ageing speed partly through exosomal miRNAs. Nature. 2017;548:52-57 pubmed publisher
Witusik Perkowska M, Zakrzewska M, Sikorska B, Papierz W, Jaskolski D, Szemraj J, et al. Glioblastoma-derived cells in vitro unveil the spectrum of drug resistance capability - comparative study of tumour chemosensitivity in different culture systems. Biosci Rep. 2017;37: pubmed publisher
Wang L, Zhang T, Wang L, Cai Y, Zhong X, He X, et al. Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission. EMBO J. 2017;36:1330-1347 pubmed publisher
Katayama M, Hirayama T, Kiyono T, Onuma M, Tani T, Takeda S, et al. Immortalized prairie vole-derived fibroblasts (VMF-K4DTs) can be transformed into pluripotent stem cells and provide a useful tool with which to determine optimal reprogramming conditions. J Reprod Dev. 2017;63:311-318 pubmed publisher
Close J, Yao Z, Levi B, Miller J, Bakken T, Menon V, et al. Single-Cell Profiling of an In Vitro Model of Human Interneuron Development Reveals Temporal Dynamics of Cell Type Production and Maturation. Neuron. 2017;93:1035-1048.e5 pubmed publisher
Liu K, Mao J, Song L, Fan A, Zhang S, Wang J, et al. DNA repair and replication links to pluripotency and differentiation capacity of pig iPS cells. PLoS ONE. 2017;12:e0173047 pubmed publisher
Choi H, Hong Y, Kim J, Song H, Cho S, Bae H, et al. In vivo differentiation of induced pluripotent stem cells into neural stem cells by chimera formation. PLoS ONE. 2017;12:e0170735 pubmed publisher
Middelkamp S, van Heesch S, Braat A, de Ligt J, van Iterson M, Simonis M, et al. Molecular dissection of germline chromothripsis in a developmental context using patient-derived iPS cells. Genome Med. 2017;9:9 pubmed publisher
Kwan J, Sczaniecka A, Heidary Arash E, Nguyen L, Chen C, Ratkovic S, et al. DLG5 connects cell polarity and Hippo signaling protein networks by linking PAR-1 with MST1/2. Genes Dev. 2016;30:2696-2709 pubmed publisher
Dvorakova M, Jahan I, Macova I, Chumak T, Bohuslavova R, Syka J, et al. Incomplete and delayed Sox2 deletion defines residual ear neurosensory development and maintenance. Sci Rep. 2016;6:38253 pubmed publisher
Kaufhold S, Garban H, Bonavida B. Yin Yang 1 is associated with cancer stem cell transcription factors (SOX2, OCT4, BMI1) and clinical implication. J Exp Clin Cancer Res. 2016;35:84 pubmed publisher
Shu L, Sun W, Li L, Xu Z, Lin L, Xie P, et al. Genome-wide alteration of 5-hydroxymenthylcytosine in a mouse model of Alzheimer's disease. BMC Genomics. 2016;17:381 pubmed publisher
Kuse Y, Tsuruma K, Sugitani S, Izawa H, Ohno Y, Shimazawa M, et al. Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina. Sci Rep. 2016;6:23811 pubmed publisher
Uda Y, Xu S, Matsumura T, Takei Y. P2Y4 Nucleotide Receptor in Neuronal Precursors Induces Glutamatergic Subtype Markers in Their Descendant Neurons. Stem Cell Reports. 2016;6:474-482 pubmed publisher
Islam M, Smith D, Niu W, Fang S, Iqbal N, Sun G, et al. Enhancer Analysis Unveils Genetic Interactions between TLX and SOX2 in Neural Stem Cells and In Vivo Reprogramming. Stem Cell Reports. 2015;5:805-15 pubmed
Kotterman M, Vazin T, Schaffer D. Enhanced selective gene delivery to neural stem cells in vivo by an adeno-associated viral variant. Development. 2015;142:1885-92 pubmed publisher
product information
Catalog Number :
AB5603
Subcategory :
Neuroscience
Product Name :
Anti-Sox2 Antibody
Product Type :
Antibodies
Clonality :
Polyclonal Antibody
Gene ID :
P48431
Host Name :
Rabbit
Antigen :
Sox2
Conjugate :
Affinity Purified
Product Description :
Anti-Sox2 Antibody
Cross Reactivity :
Human;Mouse
Background :
SOX 2 is also known as SRY related HMG BOX gene 2. All SOX proteins have a single HMG box and bind linear DNA in a sequence-specific manner, resulting in the bending of DNA through large angles. Bending causes the DNA helix to open for some distance, which may affect binding and interactions of other transcription factors. SOX1, SOX2 and SOX3 show the closest homology to SRY. They share maximum homology within the HMG domain and are expressed mainly in the developing nervous system of the mouse. These genes share significant homology outside the HMG box also and are highly conserved throughout their evolution.
Immunogen :
KLH-conjugated linear peptide corresponding to human Sox2 RBX.
Specificity :
This antibody recognizes Sox2
Package Size :
100 μg
Uses :
Immunocytochemistry;Western Blotting
Storage :
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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