Glial Fibrillary Acidic Protein Antibody | EMD Millipore AB5541 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Glial Fibrillary Acidic Protein Antibody

catalog :

AB5541

quantity :

50 µL

clonality :

polyclonal

host :

chicken

conjugate :

nonconjugated

reactivity :

human, mouse, rat, bovine

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

citations: 33

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - free floating section; rat; 1:1000; loading ...; fig 1a, 4m	Stein L, Lhamo R, Cao A, Workinger J, TINSLEY I, Doyle R, et al. Dorsal vagal complex and hypothalamic glia differentially respond to leptin and energy balance dysregulation. Transl Psychiatry. 2020;10:90 pubmed publisher
immunohistochemistry; mouse; 1:1000; loading ...; fig 1b	Wu Z, Parry M, Hou X, Liu M, Wang H, Cain R, et al. Gene therapy conversion of striatal astrocytes into GABAergic neurons in mouse models of Huntington's disease. Nat Commun. 2020;11:1105 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 5a	Holmkvist A, Agorelius J, Forni M, Nilsson U, Linsmeier C, Schouenborg J. Local delivery of minocycline-loaded PLGA nanoparticles from gelatin-coated neural implants attenuates acute brain tissue responses in mice. J Nanobiotechnology. 2020;18:27 pubmed publisher
immunocytochemistry; mouse; 1:600; loading ...; fig 1d	Montalbán Loro R, Lozano Ureña A, Ito M, Krueger C, Reik W, Ferguson Smith A, et al. TET3 prevents terminal differentiation of adult NSCs by a non-catalytic action at Snrpn. Nat Commun. 2019;10:1726 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 1b	Nam M, Han K, Lee J, Bae J, An H, Park S, et al. Expression of µ-Opioid Receptor in CA1 Hippocampal Astrocytes. Exp Neurobiol. 2018;27:120-128 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; fig s2k	Dias D, Kim H, Holl D, Werne Solnestam B, Lundeberg J, Carlen M, et al. Reducing Pericyte-Derived Scarring Promotes Recovery after Spinal Cord Injury. Cell. 2018;173:153-165.e22 pubmed publisher
immunohistochemistry; mouse; 1:600; fig 2d	Paul A, Chaker Z, Doetsch F. Hypothalamic regulation of regionally distinct adult neural stem cells and neurogenesis. Science. 2017;356:1383-1386 pubmed publisher
immunocytochemistry; human; 1:500; tbl s1	Yasui T, Uezono N, Nakashima H, Noguchi H, Matsuda T, Noda Andoh T, et al. Hypoxia Epigenetically Confers Astrocytic Differentiation Potential on Human Pluripotent Cell-Derived Neural Precursor Cells. Stem Cell Reports. 2017;8:1743-1756 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 2a	Wizeman J, Mohan R. Expression of peptidylarginine deiminase 4 in an alkali injury model of retinal gliosis. Biochem Biophys Res Commun. 2017;487:134-139 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:2000; loading ...; fig 1c immunohistochemistry - paraffin section; human; 1:2000; loading ...; fig 1b	Sha L, Wang X, Li J, Shi X, Wu L, Shen Y, et al. Pharmacologic inhibition of Hsp90 to prevent GLT-1 degradation as an effective therapy for epilepsy. J Exp Med. 2017;214:547-563 pubmed publisher
immunohistochemistry; mouse; 1:1000; loading ...; fig 4a	Gadani S, Smirnov I, Smith A, Overall C, Kipnis J. Characterization of meningeal type 2 innate lymphocytes and their response to CNS injury. J Exp Med. 2017;214:285-296 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:300; loading ...; fig 5a	Yang S, Lee D, Shin J, Lee S, Baek S, Kim J, et al. Nec-1 alleviates cognitive impairment with reduction of Aβ and tau abnormalities in APP/PS1 mice. EMBO Mol Med. 2017;9:61-77 pubmed publisher
immunohistochemistry; mouse; 1:1000; loading ...; fig 5e	Yim N, Ryu S, Choi K, Lee K, Lee S, Choi H, et al. Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module. Nat Commun. 2016;7:12277 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; fig 1	Almad A, Doreswamy A, Gross S, Richard J, Huo Y, Haughey N, et al. Connexin 43 in astrocytes contributes to motor neuron toxicity in amyotrophic lateral sclerosis. Glia. 2016;64:1154-69 pubmed publisher
immunohistochemistry; mouse; 1:100; fig s4 immunohistochemistry; human; 1:100; fig 1	Liu Q, Sanai N, Jin W, La Cava A, Van Kaer L, Shi F. Neural stem cells sustain natural killer cells that dictate recovery from brain inflammation. Nat Neurosci. 2016;19:243-52 pubmed publisher
immunohistochemistry - paraffin section; human; fig 2	Cook T, Hoekstra J, Eaton D, Zhang J. Mortalin is Expressed by Astrocytes and Decreased in the Midbrain of Parkinson's Disease Patients. Brain Pathol. 2016;26:75-81 pubmed publisher
	He X, Chen W, Liu Z, Yu G, Chen Y, Cai Y, et al. Efficient and risk-reduced genome editing using double nicks enhanced by bacterial recombination factors in multiple species. Nucleic Acids Res. 2020;48:e57 pubmed publisher
	Han Y, Kwon J, Won J, An H, Jang M, Woo J, et al. Tweety-homolog (Ttyh) Family Encodes the Pore-forming Subunits of the Swelling-dependent Volume-regulated Anion Channel (VRACswell) in the Brain. Exp Neurobiol. 2019;28:183-215 pubmed publisher
	Smith A, Duan T, Verkman A. Aquaporin-4 reduces neuropathology in a mouse model of Alzheimer's disease by remodeling peri-plaque astrocyte structure. Acta Neuropathol Commun. 2019;7:74 pubmed publisher
	Woo D, Bae J, Nam M, An H, Ju Y, Won J, et al. Activation of Astrocytic μ-opioid Receptor Elicits Fast Glutamate Release Through TREK-1-Containing K2P Channel in Hippocampal Astrocytes. Front Cell Neurosci. 2018;12:319 pubmed publisher
	Augé E, Pelegri C, Manich G, Cabezón I, Guinovart J, Duran J, et al. Astrocytes and neurons produce distinct types of polyglucosan bodies in Lafora disease. Glia. 2018;66:2094-2107 pubmed publisher
	Rayasam A, Hsu M, Kijak J, Kissel L, Hernandez G, Sandor M, et al. Immune responses in stroke: how the immune system contributes to damage and healing after stroke and how this knowledge could be translated to better cures?. Immunology. 2018;154:363-376 pubmed publisher
	Xu M, Ouyang Q, Gong J, Pescosolido M, Pruett B, Mishra S, et al. Mixed Neurodevelopmental and Neurodegenerative Pathology in Nhe6-Null Mouse Model of Christianson Syndrome. Eneuro. 2017;4: pubmed publisher
	Hu J, Dziumbla S, Lin J, Bibli S, Zukunft S, de Mos J, et al. Inhibition of soluble epoxide hydrolase prevents diabetic retinopathy. Nature. 2017;552:248-252 pubmed publisher
	Tang Y, Lin Y, Ni H, Dong J, Yuan H, Zhang Y, et al. Inhibiting Histone Deacetylase 2 (HDAC2) Promotes Functional Recovery From Stroke. J Am Heart Assoc. 2017;6: pubmed publisher
	Ardelt A, Carpenter R, Iwuchukwu I, Zhang A, Lin W, Kosciuczuk E, et al. Transgenic expression of HuR increases vasogenic edema and impedes functional recovery in rodent ischemic stroke. Neurosci Lett. 2017;661:126-131 pubmed publisher
	McFerrin J, Patton B, Sunderhaus E, Kretzschmar D. NTE/PNPLA6 is expressed in mature Schwann cells and is required for glial ensheathment of Remak fibers. Glia. 2017;65:804-816 pubmed publisher
	Ashley S, Pretto C, Stier M, Kadiyala P, Castro Jorge L, Hsu T, et al. Matrix Metalloproteinase Activity in Infections by an Encephalitic Virus, Mouse Adenovirus Type 1. J Virol. 2017;91: pubmed publisher
	Sant Anna M, Kusuda R, Bozzo T, Bassi G, Alves Filho J, Cunha F, et al. Medial plantar nerve ligation as a novel model of neuropathic pain in mice: pharmacological and molecular characterization. Sci Rep. 2016;6:26955 pubmed publisher
	Daschil N, Humpel C. Green-Fluorescent Protein(+) Astrocytes Attach to Beta-Amyloid Plaques in an Alzheimer Mouse Model and Are Sensitive for Clasmatodendrosis. Front Aging Neurosci. 2016;8:75 pubmed publisher
	Lavrov I, Fox L, Shen J, Han Y, Cheng J. Gap Junctions Contribute to the Regulation of Walking-Like Activity in the Adult Mudpuppy (Necturus Maculatus). PLoS ONE. 2016;11:e0152650 pubmed publisher
	An H, Cho D, Lee S, Yang Y, Han S, Lee C. Differential Cellular Tropism of Lentivirus and Adeno-Associated Virus in the Brain of Cynomolgus Monkey. Exp Neurobiol. 2016;25:48-54 pubmed publisher
	Krey L, LÃ¼hder F, Kusch K, Czech Zechmeister B, KÃ¶nnecke B, Fleming Outeiro T, et al. Knockout of silent information regulator 2 (SIRT2) preserves neurological function after experimental stroke in mice. J Cereb Blood Flow Metab. 2015;35:2080-8 pubmed publisher

product information

Catalog Number :

AB5541

Subcategory :

Neuroscience

Product Name :

Anti-Glial Fibrillary Acidic Protein Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P14136

Host Name :

Chicken

Antigen :

Glial Fibrillary Acidic Protein

Conjugate :

Affinity Purified

Product Description :

Anti-Glial Fibrillary Acidic Protein Antibody

Cross Reactivity :

Human;Mouse;Rat;Bovine;Pig

Background :

Glial fibrillary acidic protein (GFAP) is a class-III intermediate filament. Type III intermediate filaments contain three domains, the most conserved of which is the rod domain. The specific sequence for this region of the protein may differ between the different intermediate filament genes for type III proteins, but the structure of the protein is highly conserved. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.

ALT Names :

GFAP

Immunogen :

Purified bovine GFAP.

Specificity :

Glial fibrillary acidic protein (GFAP). Reacts with both native and recombinant protein.

Package Size :

50 µL

Uses :

Immunocytochemistry;Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting

Storage :

Stable for 1 year at -20°C in undiluted aliquots from date of receipt. Do not store in a self-defrosting freezer. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.