Opsin Antibody, blue | EMD Millipore AB5407 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Opsin Antibody, blue

catalog :

AB5407

quantity :

100 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 13

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; human; 1:200; loading ...; fig 2e	Voigt A, Binkley E, Flamme Wiese M, Zeng S, DeLuca A, Scheetz T, et al. Single-Cell RNA Sequencing in Human Retinal Degeneration Reveals Distinct Glial Cell Populations. Cells. 2020;9: pubmed publisher
immunohistochemistry - frozen section; human; 1:100; loading ...; fig 1	Kamar S, Howlett M, Klooster J, Graaff W, Csikós T, Rabelink M, et al. Degenerated Cones in Cultured Human Retinas Can Successfully Be Optogenetically Reactivated. Int J Mol Sci. 2020;21: pubmed publisher
immunocytochemistry; human; 1:1000; fig 4	Wiley L, Burnight E, DeLuca A, Anfinson K, Cranston C, Kaalberg E, et al. cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness. Sci Rep. 2016;6:30742 pubmed publisher
immunohistochemistry; mouse; 1:1000; fig 3	Jain V, Srivastava I, Palchaudhuri S, Goel M, Sinha Mahapatra S, Dhingra N. Classical Photoreceptors Are Primarily Responsible for the Pupillary Light Reflex in Mouse. PLoS ONE. 2016;11:e0157226 pubmed publisher
immunohistochemistry - paraffin section; mouse; fig 6	Ji X, Liu Y, Hurd R, Wang J, Fitzmaurice B, Nishina P, et al. Retinal Pigment Epithelium Atrophy 1 (rpea1): A New Mouse Model With Retinal Detachment Caused by a Disruption of Protein Kinase C, Î¸. Invest Ophthalmol Vis Sci. 2016;57:877-88 pubmed publisher
immunocytochemistry; human; 1:200; tbl 1	de Souza C, Nivison Smith L, Christie D, Polkinghorne P, McGhee C, Kalloniatis M, et al. Macromolecular markers in normal human retina and applications to human retinal disease. Exp Eye Res. 2016;150:135-48 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 1h	Grishchuk Y, Stember K, Matsunaga A, Olivares A, CRUZ N, King V, et al. Retinal Dystrophy and Optic Nerve Pathology inÂ the Mouse Model of Mucolipidosis IV. Am J Pathol. 2016;186:199-209 pubmed publisher
	Schwarz C, Sharma R, Cheong S, Keller M, Williams D, Hunter J. Selective S Cone Damage and Retinal Remodeling Following Intense Ultrashort Pulse Laser Exposures in the Near-Infrared. Invest Ophthalmol Vis Sci. 2018;59:5973-5984 pubmed publisher
	Deng W, Li J, Zhu P, Chiodo V, Smith W, Freedman B, et al. Human L- and M-opsins restore M-cone function in a mouse model for human blue cone monochromacy. Mol Vis. 2018;24:17-28 pubmed
	Lv J, Zhou G, Chen X, Chen H, Wu K, Xiang L, et al. Targeted RP9 ablation and mutagenesis in mouse photoreceptor cells by CRISPR-Cas9. Sci Rep. 2017;7:43062 pubmed publisher
	Gianesini C, Hiragaki S, Laurent V, Hicks D, Tosini G. Cone Viability Is Affected by Disruption of Melatonin Receptors Signaling. Invest Ophthalmol Vis Sci. 2016;57:94-104 pubmed publisher
	Jimeno D, Gómez C, Calzada N, de la Villa P, Lillo C, Santos E. RASGRF2 controls nuclear migration in postnatal retinal cone photoreceptors. J Cell Sci. 2016;129:729-42 pubmed publisher
	Choi V, Bigelow C, McGee T, Gujar A, Li H, Hanks S, et al. AAV-mediated RLBP1 gene therapy improves the rate of dark adaptation in Rlbp1 knockout mice. Mol Ther Methods Clin Dev. 2015;2:15022 pubmed publisher

product information

Catalog Number :

AB5407

Subcategory :

Neuroscience

Product Name :

Anti-Opsin Antibody, blue

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P03999

Host Name :

Rabbit

Antigen :

Opsin

Conjugate :

Purified

Product Description :

Anti-Opsin Antibody, blue

Cross Reactivity :

Human;Mouse;Monkey

Background :

The full range of color discrimination in humans is based on the presence and function of three cone photoreceptor mechanisms. Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein, which gives sensitivity in the short (S cone, peak sensitivity about 420nm), middle (M cone, peak sensitivity about 530nm with polymorphism; Winderckx et al., 1993; Neitz & Neitz, 1998), and long (L cone, peak sensitivity about 560nm with polymorphism; Neitz & Jacobs, 1990) wavelengths of the light spectrum. All three opsins are transmembrane proteins with seven membrane-spanning regions. Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation. Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event (Nathans, 1989; Nathans et al., 1992). The S cone (blue) opsin is located on chromosome 7 and seems to have stronger conservation with rhodopsin. Cone photoreceptor distribution in humans is dominated by the M and L cone pigments.

Immunogen :

Recombinant human blue opsin.

Specificity :

Recognizes Opsin, blue.

Package Size :

100 μg

Uses :

Immunohistochemistry (Paraffin)

Storage :

Maintain for 1 year at 2–8°C from date of shipment.