Opsin Antibody, Red/Green | EMD Millipore AB5405 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Opsin Antibody, Red/Green

catalog :

AB5405

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 29

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:300; loading ...; fig 8s2j immunohistochemistry; mouse; 1:300; loading ...; fig 8s4h	Vigouroux R, Cesar Q, Chedotal A, Nguyen Ba Charvet K. Revisiting the role of Dcc in visual system development with a novel eye clearing method. elife. 2020;9: pubmed publisher
immunohistochemistry - frozen section; human; 1:200; loading ...; fig 2e	Voigt A, Binkley E, Flamme Wiese M, Zeng S, DeLuca A, Scheetz T, et al. Single-Cell RNA Sequencing in Human Retinal Degeneration Reveals Distinct Glial Cell Populations. Cells. 2020;9: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 5d	Findlay A, McKie L, Keighren M, Clementson Mobbs S, Sanchez Pulido L, Wells S, et al. Fam151b, the mouse homologue of C.elegans menorin gene, is essential for retinal function. Sci Rep. 2020;10:437 pubmed publisher
immunohistochemistry - frozen section; human; 1:100; loading ...; fig 1	Kamar S, Howlett M, Klooster J, Graaff W, Csikós T, Rabelink M, et al. Degenerated Cones in Cultured Human Retinas Can Successfully Be Optogenetically Reactivated. Int J Mol Sci. 2020;21: pubmed publisher
immunohistochemistry; mouse; 1:500; fig 3	Jain V, Srivastava I, Palchaudhuri S, Goel M, Sinha Mahapatra S, Dhingra N. Classical Photoreceptors Are Primarily Responsible for the Pupillary Light Reflex in Mouse. PLoS ONE. 2016;11:e0157226 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500; fig 5	BrÃ¼ggen B, Kremser C, Bickert A, Ebel P, Vom Dorp K, Schultz K, et al. Defective ceramide synthases in mice cause reduced amplitudes in electroretinograms and altered sphingolipid composition in retina and cornea. Eur J Neurosci. 2016;44:1700-13 pubmed publisher
immunohistochemistry - paraffin section; mouse; fig 6	Ji X, Liu Y, Hurd R, Wang J, Fitzmaurice B, Nishina P, et al. Retinal Pigment Epithelium Atrophy 1 (rpea1): A New Mouse Model With Retinal Detachment Caused by a Disruption of Protein Kinase C, Î¸. Invest Ophthalmol Vis Sci. 2016;57:877-88 pubmed publisher
immunohistochemistry; mouse; fig 5	Wen B, Li S, Li H, Chen Y, Ma X, Wang J, et al. Microphthalmia-associated transcription factor regulates the visual cycle genes Rlbp1 and Rdh5 in the retinal pigment epithelium. Sci Rep. 2016;6:21208 pubmed publisher
immunocytochemistry; human; 1:200; tbl 1	de Souza C, Nivison Smith L, Christie D, Polkinghorne P, McGhee C, Kalloniatis M, et al. Macromolecular markers in normal human retina and applications to human retinal disease. Exp Eye Res. 2016;150:135-48 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 1h	Grishchuk Y, Stember K, Matsunaga A, Olivares A, CRUZ N, King V, et al. Retinal Dystrophy and Optic Nerve Pathology inÂ the Mouse Model of Mucolipidosis IV. Am J Pathol. 2016;186:199-209 pubmed publisher
	Nishiguchi K, Fujita K, Miya F, Katayama S, Nakazawa T. Single AAV-mediated mutation replacement genome editing in limited number of photoreceptors restores vision in mice. Nat Commun. 2020;11:482 pubmed publisher
	Narayan D, Ao J, Wood J, Casson R, Chidlow G. Spatio-temporal characterization of S- and M/L-cone degeneration in the Rd1 mouse model of retinitis pigmentosa. BMC Neurosci. 2019;20:46 pubmed publisher
	Cai Y, Cheng T, Yao Y, Li X, Ma Y, Li L, et al. In vivo genome editing rescues photoreceptor degeneration via a Cas9/RecA-mediated homology-directed repair pathway. Sci Adv. 2019;5:eaav3335 pubmed publisher
	Xiong W, Wu D, Xue Y, Wang S, Chung M, Ji X, et al. AAV cis-regulatory sequences are correlated with ocular toxicity. Proc Natl Acad Sci U S A. 2019;116:5785-5794 pubmed publisher
	Xu L, Kong L, Wang J, Ash J. Stimulation of AMPK prevents degeneration of photoreceptors and the retinal pigment epithelium. Proc Natl Acad Sci U S A. 2018;115:10475-10480 pubmed publisher
	Deng W, Li J, Zhu P, Chiodo V, Smith W, Freedman B, et al. Human L- and M-opsins restore M-cone function in a mouse model for human blue cone monochromacy. Mol Vis. 2018;24:17-28 pubmed
	Shindou H, Koso H, Sasaki J, Nakanishi H, Sagara H, Nakagawa K, et al. Docosahexaenoic acid preserves visual function by maintaining correct disc morphology in retinal photoreceptor cells. J Biol Chem. 2017;292:12054-12064 pubmed publisher
	Weinrich T, Powner M, Lynch A, Jonnal R, Werner J, Jeffery G. No evidence for loss of short-wavelength sensitive cone photoreceptors in normal ageing of the primate retina. Sci Rep. 2017;7:46346 pubmed publisher
	Ng L, Liu H, St Germain D, Hernandez A, Forrest D. Deletion of the Thyroid Hormone-Activating Type 2 Deiodinase Rescues Cone Photoreceptor Degeneration but Not Deafness in Mice Lacking Type 3 Deiodinase. Endocrinology. 2017;158:1999-2010 pubmed publisher
	Di Pierdomenico J, García Ayuso D, Pinilla I, Cuenca N, Vidal Sanz M, Agudo Barriuso M, et al. Early Events in Retinal Degeneration Caused by Rhodopsin Mutation or Pigment Epithelium Malfunction: Differences and Similarities. Front Neuroanat. 2017;11:14 pubmed publisher
	Lv J, Zhou G, Chen X, Chen H, Wu K, Xiang L, et al. Targeted RP9 ablation and mutagenesis in mouse photoreceptor cells by CRISPR-Cas9. Sci Rep. 2017;7:43062 pubmed publisher
	Kim K, Park S, Kim J, Lee S, Kim D, Koo T, et al. Genome surgery using Cas9 ribonucleoproteins for the treatment of age-related macular degeneration. Genome Res. 2017;27:419-426 pubmed publisher
	Shin Y, Moiseyev G, Chakraborty D, Ma J. A Dominant Mutation in Rpe65, D477G, Delays Dark Adaptation and Disturbs the Visual Cycle in the Mutant Knock-In Mice. Am J Pathol. 2017;187:517-527 pubmed publisher
	Wiley L, Burnight E, DeLuca A, Anfinson K, Cranston C, Kaalberg E, et al. cGMP production of patient-specific iPSCs and photoreceptor precursor cells to treat retinal degenerative blindness. Sci Rep. 2016;6:30742 pubmed publisher
	Jones B, Pfeiffer R, Ferrell W, Watt C, Tucker J, Marc R. Retinal Remodeling and Metabolic Alterations in Human AMD. Front Cell Neurosci. 2016;10:103 pubmed publisher
	Appelbaum T, Becker D, Santana E, Aguirre G. Molecular studies of phenotype variation in canine RPGR-XLPRA1. Mol Vis. 2016;22:319-31 pubmed
	Gianesini C, Hiragaki S, Laurent V, Hicks D, Tosini G. Cone Viability Is Affected by Disruption of Melatonin Receptors Signaling. Invest Ophthalmol Vis Sci. 2016;57:94-104 pubmed publisher
	Jimeno D, Gómez C, Calzada N, de la Villa P, Lillo C, Santos E. RASGRF2 controls nuclear migration in postnatal retinal cone photoreceptors. J Cell Sci. 2016;129:729-42 pubmed publisher
	Choi V, Bigelow C, McGee T, Gujar A, Li H, Hanks S, et al. AAV-mediated RLBP1 gene therapy improves the rate of dark adaptation in Rlbp1 knockout mice. Mol Ther Methods Clin Dev. 2015;2:15022 pubmed publisher

product information

Catalog Number :

AB5405

Subcategory :

Neuroscience

Product Name :

Anti-Opsin Antibody, Red/Green

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P04000

Host Name :

Rabbit

Antigen :

Opsin

Conjugate :

Purified

Product Description :

Anti-Opsin Antibody, Red/Green

Cross Reactivity :

Human;Mouse;Monkey

Background :

The full range of color discrimination in humans is based on the presence and function of three cone photoreceptor mechanisms. Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein, which gives sensitivity in the short (S cone, peak sensitivity about 420nm), middle (M cone, peak sensitivity about 530nm with polymorphism; Winderckx et al., 1993; Neitz & Neitz, 1998), and long (L cone, peak sensitivity about 560nm with polymorphism; Neitz & Jacobs, 1990) wavelengths of the light spectrum. All three opsins are transmembrane proteins with seven membrane-spanning regions. Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation. Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event (Nathans, 1989; Nathans et al., 1992). The S cone (blue) opsin is located on chromosome 7 and seems to have stronger conservation with rhodopsin. Cone photoreceptor distribution in humans is dominated by the M and L cone pigments.

Immunogen :

Recombinant human red/green opsin.

Specificity :

Recognizes opsin, red/green.

Package Size :

100 µg

Uses :

Immunohistochemistry (Paraffin)

Storage :

Stable for up to 6 months at 2-8°C in undiluted from date of receipt. Do not freeze. Keep vials well sealed to prevent evaporation.