FOXO1 Antibody | EMD Millipore AB4130 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

FOXO1 Antibody

catalog :

AB4130

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

immunocytochemistry, chromatin immunoprecipitation

citations: 1

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; mouse; fig 7d	Doan K, Kinyua A, Yang D, Ko C, Moh S, Shong K, et al. FoxO1 in dopaminergic neurons regulates energy homeostasis and targets tyrosine hydroxylase. Nat Commun. 2016;7:12733 pubmed publisher

product information

Catalog Number :

AB4130

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-FOXO1 Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q12778

Host Name :

Rabbit

Antigen :

FOXO1

Conjugate :

Affinity Purified

Product Description :

Anti-FOXO1 Antibody

Cross Reactivity :

Human;Mouse;Rat

ALT Names :

Forkhead Box Protein O1; FKHR

Immunogen :

Synthetic peptide corresponding to amino acids 521-532 of human FOXO1 (SHTHPGHAQQTS).

Specificity :

Recognizes FOXO1. The calculated molecular weight is ~69.7 kDa.

Package Size :

100 µg

Uses :

Immunocytochemistry

Storage :

Maintain unopened vial at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles. The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.