Smad2 Antibody, phospho-specific (Ser465/467) | EMD Millipore AB3849 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Smad2 Antibody, phospho-specific (Ser465/467)

catalog :

AB3849

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat, chicken, zebrafish

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, other

citations: 16

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig s6a	Ruiz Gutierrez M, Bölükbaşı Ö, Alexe G, Kotini A, Ballotti K, Joyce C, et al. Therapeutic discovery for marrow failure with MDS predisposition using pluripotent stem cells. JCI Insight. 2019;5: pubmed publisher
other; human; loading ...; fig 1a	Sun H, Peng Z, Tang H, Xie D, Jia Z, Zhong L, et al. Loss of KLF4 and consequential downregulation of Smad7 exacerbate oncogenic TGF-? signaling in and promote progression of hepatocellular carcinoma. Oncogene. 2017;36:2957-2968 pubmed publisher
western blot; zebrafish ; 1:500; fig 3 western blot; human; 1:500; fig 5	Liu Z, Ning G, Xu R, Cao Y, Meng A, Wang Q. Fscn1 is required for the trafficking of TGF-β family type I receptors during endoderm formation. Nat Commun. 2016;7:12603 pubmed publisher
western blot; rat; 1:400; fig 7	Cui L, Wang Y, Yu R, Li B, Xie S, Gao Y, et al. Jia-Shen decoction-medicated serum inhibits angiotensin-II induced cardiac fibroblast proliferation via the TGF-?1/Smad signaling pathway. Mol Med Rep. 2016;14:1610-6 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:100; fig 6	Chen P, Qin L, Li G, Tellides G, Simons M. Smooth muscle FGF/TGFÎ² cross talk regulates atherosclerosis progression. EMBO Mol Med. 2016;8:712-28 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 3	Shukla P, Chaudhry K, Mir H, Gangwar R, Yadav N, Manda B, et al. Chronic ethanol feeding promotes azoxymethane and dextran sulfate sodium-induced colonic tumorigenesis potentially by enhancing mucosal inflammation. BMC Cancer. 2016;16:189 pubmed publisher
western blot; mouse; 1:500; fig 2	del RÃo C, Navarrete C, Collado J, Bellido M, GÃ³mez CaÃ±as M, Pazos M, et al. The cannabinoid quinol VCE-004.8 alleviates bleomycin-induced scleroderma and exerts potent antifibrotic effects through peroxisome proliferator-activated receptor-Î³ and CB2 pathways. Sci Rep. 2016;6:21703 pubmed publisher
immunocytochemistry; mouse; fig 5	Iyer S, Chou F, Wang R, Chiu H, Raju V, Little M, et al. Crim1 has cell-autonomous and paracrine roles during embryonic heart development. Sci Rep. 2016;6:19832 pubmed publisher
	Song B, Park S, Zhao J, Fong K, Li S, Lee Y, et al. Targeting FOXA1-mediated repression of TGF-β signaling suppresses castration-resistant prostate cancer progression. J Clin Invest. 2019;129:569-582 pubmed publisher
	Del Rio C, Cantarero I, Palomares B, Gómez Cañas M, Fernandez Ruiz J, Pavicic C, et al. VCE-004.3, a cannabidiol aminoquinone derivative, prevents bleomycin-induced skin fibrosis and inflammation through PPARγ- and CB2 receptor-dependent pathways. Br J Pharmacol. 2018;175:3813-3831 pubmed publisher
	Rodríguez Uribe G, Serafín Higuera N, Damian Morales G, Cortés Malagón E, García Hernández V, Verdejo Torres O, et al. HPV16-E6 Oncoprotein Activates TGF-β and Wnt/β-Catenin Pathways in the Epithelium-Mesenchymal Transition of Cataracts in a Transgenic Mouse Model. Biomed Res Int. 2018;2018:2847873 pubmed publisher
	Tosi G, Neri G, Caldi E, Fusco F, Bacci T, Tarantello A, et al. TGF-? concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration. Sci Rep. 2018;8:8053 pubmed publisher
	Tesseur I, Nguyen A, Chang B, Li L, Woodling N, Wyss Coray T, et al. Deficiency in Neuronal TGF-β Signaling Leads to Nigrostriatal Degeneration and Activation of TGF-β Signaling Protects against MPTP Neurotoxicity in Mice. J Neurosci. 2017;37:4584-4592 pubmed publisher
	Walker R, Czepnik M, Goebel E, McCoy J, Vujic A, Cho M, et al. Structural basis for potency differences between GDF8 and GDF11. BMC Biol. 2017;15:19 pubmed publisher
	Chaudhry S, Cain S, Morgan A, Dallas S, Shuttleworth C, Kielty C. Fibrillin-1 regulates the bioavailability of TGFbeta1. J Cell Biol. 2007;176:355-67 pubmed
	Bandyopadhyay B, Fan J, Guan S, Li Y, Chen M, Woodley D, et al. A "traffic control" role for TGFbeta3: orchestrating dermal and epidermal cell motility during wound healing. J Cell Biol. 2006;172:1093-105 pubmed

product information

Catalog Number :

AB3849

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Smad2 Antibody, phospho-specific (Ser465/467)

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q15796

Host Name :

Rabbit

Antigen :

Smad2

Conjugate :

Affinity Purified

Product Description :

Anti-Smad2 Antibody, phospho-specific (Ser465/467)

Cross Reactivity :

Human;Mouse;Rat

Background :

SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGF-β superfamily of modulators. Like many other TGF-β family members SMAD2 is involved in cell signaling. SMAD2 modulates signals of activin and TGF-β's. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.

ALT Names :

MAD, mothers against decapentaplegic homolog 2 (Drosophila);Mad protein homolog 2;Mad, mothers against decapentaplegic homolog 2;Mad-related protein 2;Mothers against DPP homolog 2;SMAD 2;SMAD family member 2;SMAD, mothers against DPP homolog 2;SMAD, mothers against DPP homolog 2 (Drosophila);Sma- and Mad-related protein 2;mother against DPP homolog 2

Immunogen :

KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser465/467 of human Smad2.

Specificity :

By western blot, the antibody recognizes a band migrating at approximately 58 kDa corresponding to Smad2 only when dually phosphorylated at Ser465 and Ser467. The antibody may also detect phosphorylated Smad3 (Ser423 and Ser425). This antibody does not cross-react with other Smad-related proteins

Package Size :

100 µL

Uses :

Immunocytochemistry;Immunofluorescence;Immunohistochemistry (Paraffin);Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt.