phospho-Smad1/Smad5/Smad8 Antibody, phospho-specific (Ser463/465)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Smad1/Smad5/Smad8 Antibody, phospho-specific (Ser463/465)

catalog :

AB3848

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, pigs

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

citations: 10

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; pigs ; 1:300; loading ...; fig 2c	Kobayashi T, Zhang H, Tang W, Irie N, Withey S, Klisch D, et al. Principles of early human development and germ cell program from conserved model systems. Nature. 2017;546:416-420 pubmed publisher
	Senft A, Costello I, King H, Mould A, Bikoff E, Robertson E. Combinatorial Smad2/3 Activities Downstream of Nodal Signaling Maintain Embryonic/Extra-Embryonic Cell Identities during Lineage Priming. Cell Rep. 2018;24:1977-1985.e7 pubmed publisher
	Mularczyk E, Singh M, Godwin A, Galli F, Humphreys N, Adamson A, et al. ADAMTS10-mediated tissue disruption in Weill-Marchesani syndrome. Hum Mol Genet. 2018;27:3675-3687 pubmed publisher
	Wang L, Chai Y, Li C, Liu H, Su W, Liu X, et al. Oxidized phospholipids are ligands for LRP6. Bone Res. 2018;6:22 pubmed publisher
	Nie X, Zheng J, Ricupero C, He L, Jiao K, Mao J. mTOR acts as a pivotal signaling hub for neural crest cells during craniofacial development. PLoS Genet. 2018;14:e1007491 pubmed publisher
	Chung M, Bujnis M, Barkauskas C, Kobayashi Y, Hogan B. Niche-mediated BMP/SMAD signaling regulates lung alveolar stem cell proliferation and differentiation. Development. 2018;145: pubmed publisher
	Tania N, Maarsingh H, T Bos I, Mattiotti A, Prakash S, Timens W, et al. Endothelial follistatin-like-1 regulates the postnatal development of the pulmonary vasculature by modulating BMP/Smad signaling. Pulm Circ. 2017;7:219-231 pubmed publisher
	Kirmizitas A, Meiklejohn S, Ciau Uitz A, Stephenson R, Patient R. Dissecting BMP signaling input into the gene regulatory networks driving specification of the blood stem cell lineage. Proc Natl Acad Sci U S A. 2017;114:5814-5821 pubmed publisher
	Li A, Figueroa S, Jiang T, Wu P, Widelitz R, Nie Q, et al. Diverse feather shape evolution enabled by coupling anisotropic signalling modules with self-organizing branching programme. Nat Commun. 2017;8:ncomms14139 pubmed publisher
	Watanabe M, Fung E, Chan F, Wong J, Coutts M, Monuki E. BMP4 acts as a dorsal telencephalic morphogen in a mouse embryonic stem cell culture system. Biol Open. 2016;5:1834-1843 pubmed publisher

product information

Catalog Number :

AB3848

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-Smad1/Smad5/Smad8 Antibody, phospho-specific (Ser463/465)

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q15797

Host Name :

Rabbit

Antigen :

phospho-Smad1/Smad5/Smad8

Conjugate :

Affinity Purified

Product Description :

Anti-phospho-Smad1/Smad5/Smad8 Antibody, phospho-specific (Ser463/465)

Cross Reactivity :

Human;Mouse;Rat;Mink;Xenopus

Background :

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD).

ALT Names :

Mothers against decapentaplegic homolog

Immunogen :

KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser463/465 of human Smad5.

Specificity :

Recognizes Smad1 only when dually phosphorylated at Ser463 and Ser465, as well as phosphorylated Smad5 and Smad8 at the equivalent sites. This antibody does not crossreact with other Smad-related proteins.

Package Size :

100 µL

Uses :

Flow Cytometry;Immunocytochemistry;Immunohistochemistry (Paraffin);Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Do not aliquot antibody. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.