Prosurfactant Protein C (proSP-C) Antibody | EMD Millipore AB3786 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Prosurfactant Protein C (proSP-C) Antibody

catalog :

AB3786

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 29

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig 3e	Zhang K, Yao E, Lin C, Chou Y, Wong J, Li J, et al. A mammalian Wnt5a-Ror2-Vangl2 axis controls the cytoskeleton and confers cellular properties required for alveologenesis. elife. 2020;9: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig s3f	Li A, Herbst R, Canner D, Schenkel J, Smith O, Kim J, et al. IL-33 Signaling Alters Regulatory T Cell Diversity in Support of Tumor Development. Cell Rep. 2019;29:2998-3008.e8 pubmed publisher
western blot; mouse; 1:5000; loading ...; fig s1	Momcilovic M, Jones A, Bailey S, Waldmann C, Li R, Lee J, et al. In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer. Nature. 2019;575:380-384 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig 1b	Chung M, Bujnis M, Barkauskas C, Kobayashi Y, Hogan B. Niche-mediated BMP/SMAD signaling regulates lung alveolar stem cell proliferation and differentiation. Development. 2018;145: pubmed publisher
immunohistochemistry - paraffin section; mouse; loading ...; fig 2c	Jamsai D, Watkins D, O Connor A, Merriner D, Gursoy S, Bird A, et al. In vivo evidence that RBM5 is a tumour suppressor in the lung. Sci Rep. 2017;7:16323 pubmed publisher
immunohistochemistry; mouse; 1:400; loading ...; fig 2d	Lin C, Yao E, Zhang K, Jiang X, Croll S, Thompson Peer K, et al. YAP is essential for mechanical force production and epithelial cell proliferation during lung branching morphogenesis. elife. 2017;6: pubmed publisher
immunohistochemistry - paraffin section; human; 1:1000; loading ...; fig 1a immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 4c	Biasin V, Wygrecka M, Marsh L, Becker Pauly C, Brcic L, Ghanim B, et al. Meprin β contributes to collagen deposition in lung fibrosis. Sci Rep. 2017;7:39969 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:300; loading ...; fig e1d	Nonomura K, Woo S, Chang R, Gillich A, Qiu Z, Francisco A, et al. Piezo2 senses airway stretch and mediates lung inflation-induced apnoea. Nature. 2017;541:176-181 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 1c	Ray S, Chiba N, Yao C, Guan X, McConnell A, Brockway B, et al. Rare SOX2+ Airway Progenitor Cells Generate KRT5+ Cells that Repopulate Damaged Alveolar Parenchyma following Influenza Virus Infection. Stem Cell Reports. 2016;7:817-825 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig s1b	Snitow M, Lu M, Cheng L, Zhou S, Morrisey E. Ezh2 restricts the smooth muscle lineage during mouse lung mesothelial development. Development. 2016;143:3733-3741 pubmed
immunohistochemistry - paraffin section; human; 1:100; fig 7	Smirnova N, Schamberger A, Nayakanti S, Hatz R, Behr J, Eickelberg O. Detection and quantification of epithelial progenitor cell populations in human healthy and IPF lungs. Respir Res. 2016;17:83 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:2000; fig 3	Antony N, McDougall A, Mantamadiotis T, Cole T, Bird A. Creb1 regulates late stage mammalian lung development via respiratory epithelial and mesenchymal-independent mechanisms. Sci Rep. 2016;6:25569 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:2000; loading ...; fig 5b	Yin Y, Ren X, Smith C, Guo Q, Malabunga M, Guernah I, et al. Inhibition of fibroblast growth factor receptor 3-dependent lung adenocarcinoma with a human monoclonal antibody. Dis Model Mech. 2016;9:563-71 pubmed publisher
immunohistochemistry - paraffin section; rat; 1:200; loading ...; fig 1b	Kato T, Oka K, Nakamura T, Ito A. Decreased expression of Met during differentiation in rat lung. Eur J Histochem. 2016;60:2575 pubmed publisher
	Larson Casey J, Vaid M, Gu L, He C, Cai G, Ding Q, et al. Increased flux through the mevalonate pathway mediates fibrotic repair without injury. J Clin Invest. 2019;129:4962-4978 pubmed publisher
	Gu L, Larson Casey J, Andrabi S, Lee J, Meza Perez S, Randall T, et al. Mitochondrial calcium uniporter regulates PGC-1α expression to mediate metabolic reprogramming in pulmonary fibrosis. Redox Biol. 2019;26:101307 pubmed publisher
	Katsura H, Kobayashi Y, Tata P, Hogan B. IL-1 and TNFα Contribute to the Inflammatory Niche to Enhance Alveolar Regeneration. Stem Cell Reports. 2019;12:657-666 pubmed publisher
	Henderson M, Sengupta M, McGeary I, Zhang B, Olufemi M, Brown H, et al. LRRK2 inhibition does not impart protection from α-synuclein pathology and neuron death in non-transgenic mice. Acta Neuropathol Commun. 2019;7:28 pubmed publisher
	Mollaoglu G, Jones A, Wait S, Mukhopadhyay A, Jeong S, Arya R, et al. The Lineage-Defining Transcription Factors SOX2 and NKX2-1 Determine Lung Cancer Cell Fate and Shape the Tumor Immune Microenvironment. Immunity. 2018;49:764-779.e9 pubmed publisher
	van Batenburg A, Kazemier K, Peeters T, Van Oosterhout M, van der Vis J, Grutters J, et al. Cell Type-Specific Quantification of Telomere Length and DNA Double-strand Breaks in Individual Lung Cells by Fluorescence In Situ Hybridization and Fluorescent Immunohistochemistry. J Histochem Cytochem. 2018;66:485-495 pubmed publisher
	Zheng Y, Trivedi T, Lin R, Fong Yee C, Nolte R, Manibo J, et al. Loss of the vitamin D receptor in human breast and prostate cancers strongly induces cell apoptosis through downregulation of Wnt/β-catenin signaling. Bone Res. 2017;5:17023 pubmed publisher
	Nieto P, Ambrogio C, Esteban Burgos L, Gomez Lopez G, Blasco M, Yao Z, et al. A Braf kinase-inactive mutant induces lung adenocarcinoma. Nature. 2017;548:239-243 pubmed publisher
	Jiang C, Liu G, LUCKHARDT T, Antony V, Zhou Y, Carter A, et al. Serpine 1 induces alveolar type II cell senescence through activating p53-p21-Rb pathway in fibrotic lung disease. Aging Cell. 2017;16:1114-1124 pubmed publisher
	McClendon J, Jansing N, Redente E, Gandjeva A, Ito Y, Colgan S, et al. Hypoxia-Inducible Factor 1? Signaling Promotes Repair of the Alveolar Epithelium after Acute Lung Injury. Am J Pathol. 2017;187:1772-1786 pubmed publisher
	Zhang Z, Newton K, Kummerfeld S, Webster J, Kirkpatrick D, Phu L, et al. Transcription factor Etv5 is essential for the maintenance of alveolar type II cells. Proc Natl Acad Sci U S A. 2017;114:3903-3908 pubmed publisher
	Walter D, Venancio O, Buza E, Tobias J, Deshpande C, Gudiel A, et al. Systematic In Vivo Inactivation of Chromatin-Regulating Enzymes Identifies Setd2 as a Potent Tumor Suppressor in Lung Adenocarcinoma. Cancer Res. 2017;77:1719-1729 pubmed publisher
	Laresgoiti U, Nikolić M, Rao C, Brady J, Richardson R, Batchen E, et al. Lung epithelial tip progenitors integrate glucocorticoid- and STAT3-mediated signals to control progeny fate. Development. 2016;143:3686-3699 pubmed
	Xie T, Liang J, Liu N, Huan C, Zhang Y, Liu W, et al. Transcription factor TBX4 regulates myofibroblast accumulation and lung fibrosis. J Clin Invest. 2016;126:3063-79 pubmed publisher
	Avgousti D, Herrmann C, Kulej K, Pancholi N, Sekulic N, Petrescu J, et al. A core viral protein binds host nucleosomes to sequester immune danger signals. Nature. 2016;535:173-7 pubmed

product information

Catalog Number :

AB3786

Subcategory :

Metabolism

Product Name :

Anti-Prosurfactant Protein C (proSP-C) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P11686

Host Name :

Rabbit

Antigen :

Prosurfactant Protein C (proSP-C)

Conjugate :

Serum

Product Description :

Anti-Prosurfactant Protein C (proSP-C) Antibody

Cross Reactivity :

Human;Mouse;Rat

Background :

Pulmonary surfactant is a complex mixture of phospholipids and proteins secreted from airway epithelial cells (type II cells) in alveoli which reduces the surface tension at the alveolar air liquid interface, providing alveolar stability necessary for normal ventilation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C and D. SP A (28 - 36 kDa) and SP D (43 kDa) are collagenous carbohydrate binding proteins, whereas SP B (8 - 9 kDa) and SP C (4 kDa) are non collagenous hydrophobic proteins. SP C is a fragment of a much larger precursor protein of 21 kDa. The precursor contains an exceedingly hydrophobic region of 34 amino acids that comprises most of the mature SP C, and a unique poly-valine domain.

ALT Names :

proSP-C

Immunogen :

A synthetic peptide corresponding to amino acids 1-32 from the N-terminal of the human SP-C proprotein coupled to KLH

Specificity :

Reacts strongly with proSP-C from human and mouse. Immunoprecipitates the 21 kDa proprotein as well as processing intermediates of M.W. 26 kDa, 21 kDa, 16 kDa and 14 kDa. Preincubation of the antiserum with the fusion protein results in ablation of immunostaining. On human tissue immunostains alveolar Type II cells in normal lung. The antibody also recognizes the aberrant form of proSP-C (6-12 kDa peptide) found in BAL and in alveolar proteinosis material on sections of lung samples from human patients with hereditary SP-B deficiency. On mouse tissue immunostains alveolar Type II cells in normal lung.

Package Size :

100 µL

Uses :

ELISA;Immunohistochemistry;Immunohistochemistry (Paraffin);Western Blotting

Storage :

Stable at -20ºC in undiluted aliquots for up to twelve months after date of receipt. Avoid repeated freeze/thaw cycles.