PARP (214/215) cleavage site Antibody | EMD Millipore AB3565 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PARP (214/215) cleavage site Antibody

catalog :

AB3565

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry

citations: 2

Reference
Stafman L, Mruthyunjayappa S, Waters A, Garner E, Aye J, Stewart J, et al. Targeting PIM kinase as a therapeutic strategy in human hepatoblastoma. Oncotarget. 2018;9:22665-22679 pubmed publisher
Ferrara M, Sessa G, Fiore M, Bernard F, Asteriti I, Cundari E, et al. Small molecules targeted to the microtubule-Hec1 interaction inhibit cancer cell growth through microtubule stabilization. Oncogene. 2018;37:231-240 pubmed publisher

product information

Catalog Number :

AB3565

Subcategory :

Apoptosis & Cancer

Product Name :

Anti-PARP (214/215) cleavage site Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P09874

Host Name :

Rabbit

Antigen :

PARP (214/215) cleavage site

Conjugate :

Affinity Purified

Product Description :

Anti-PARP (214/215) cleavage site Antibody

Cross Reactivity :

Human;Mouse

Background :

Poly (ADP-Ribose) Polymerase (PARP) is a 116 kDa nuclear protein which is strongly activated by binding to DNA strand breaks. PARP plays a role in DNA repair as well as in other cellular processes, including DNA replication, cell proliferation and differentiation. During apoptosis, ICE family members, such as caspase-3 and -7, cleave PARP to yield an 85 kDa and a 25 kDa fragment. PARP cleavage is considered to be one of the classical characteristics of apoptosis. This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as a marker for detecting apoptotic cells.

ALT Names :

Poly [ADP-ribose] polymerase 1;PARP-1;NAD(+) ADP-ribosyltransferase 1;ADPRT 1;Poly[ADP-ribose] synthase 1;ARTD1

Immunogen :

The antiserum was produced against a chemically synthesized peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP.

Specificity :

Human and mouse (86% conserved) PARP. Hamster and rat (86% conserved) PARP have not been tested, but are expected to react.

Package Size :

100 µL

Uses :

Western Blotting;Immunocytochemistry

Storage :

Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.