Sodium Antibody, Potassium, Chloride Cotransporter 1 | EMD Millipore AB3560P product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Sodium Antibody, Potassium, Chloride Cotransporter 1

catalog :

AB3560P

quantity :

50 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry

product information

Catalog Number :

AB3560P

Subcategory :

Neuroscience

Product Name :

Anti-Sodium Antibody, Potassium, Chloride Cotransporter 1

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P55012

Host Name :

Rabbit

Antigen :

Sodium

Conjugate :

Affinity Purified

Product Description :

Anti-Sodium Antibody, Potassium, Chloride Cotransporter 1

Cross Reactivity :

Rat;Mouse;Human;Bovine

Background :

The Na+/K+/Cl- Cotransporter isoform 1 (NKCC1) is involved in the transport of sodium, potassium, and chloride ions across membranes. NKCC1 mediates sodium and chloride reabsorption and plays an important role in cell volume regulation. NKCC1 is also involved in the control of normal cell proliferation, while its overexpression results in apparent cell transformation, in a manner similar to some protooncogenes (Panet, 1999). NKCC1 is inhibited by bumetanide and is also referred to as the bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1. Inhibition of NKCC1 activity by bumetanide decreases the high-[K+]o-mediated uptake of Cl , blocks astrocyte swelling, and leads to cell shrinkage under isotonic conditions in cells such as retinal pigment epithelia, ventricular myocytes, and vascular endothelial cells (Russell, 2000; Su, 2002). NKCC1 could contribute to an overload of intracellular Na+, K+, and Cl- and cell swelling in conditions such as cerebral ischemia (Yan, 2001). NKCC1 is expressed in multiple tissues with the amount of mRNA expression varying as much as thirty-fold. It is highly expressed in neurons and astrocytes through-out the brain and has been implicated in ischemic necrotic cell death.

ALT Names :

Basolateral Na-K-Cl symporter;Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1;solute carrier family 12 sodium/potassium/chloride transporters), member 2

Immunogen :

A 22 amino acid peptide sequence near the C-terminus of rat NKCC1. (Moore-Hoon et al. 1998; Delpire et al. 1994; Payne et al. 1995; Isering et al. 1997; Mount et al. 1998).

Specificity :

Recognizes rat Sodium, Potassium, Chloride Cotransporter 1 (NKCC1, BSC2, CCC1). The immunogen shows no significant sequence homology with other CCC.

Package Size :

50 μg

Uses :

ELISA;Immunohistochemistry;Western Blotting

Storage :

Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.