Actin γ Antibody | EMD Millipore AB3265 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Actin γ Antibody

catalog :

AB3265

quantity :

100 μL

clonality :

polyclonal

host :

domestic sheep

conjugate :

nonconjugated

reactivity :

human, Xenopus laevis

application :

western blot, immunohistochemistry, immunocytochemistry

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; Xenopus laevis; loading ...; fig 3a	Cagnetta R, Frese C, Shigeoka T, Krijgsveld J, Holt C. Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome. Neuron. 2018;99:29-46.e4 pubmed publisher
	Shi Z, Rockey D. Upregulation of the actin cytoskeleton via myocardin leads to increased expression of type 1 collagen. Lab Invest. 2017;97:1412-1426 pubmed publisher

product information

Catalog Number :

AB3265

Subcategory :

Cell Structure

Product Name :

Anti-Actin γ Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P63261

Host Name :

Sheep

Antigen :

Actin γ

Conjugate :

Serum

Product Description :

Anti-Actin γ Antibody

Cross Reactivity :

Human;Mouse;Rat

Background :

Actin is a 43kDa highly conserved structural protein. There are three main actin isotypes (alpha,beta and gamma) which show 90% amino-acid (aa) homology between isotypes and 98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. {Pardo et al, 1983, Cell 32:1093-1103 & Otey, CA et al 1986 J Cell Biol 102:1726-1737, Bassell et al, 1998, J Neurosci 18:251-265 {available online}. Furthermore, there are at least six actin isoforms in vertebrates: cardiac alpha-actin and skeletal-alpha actin (expressed in striated muscle), vascular alpha-actin and enteric gamma-actin (expressed in smooth muscle), and cytoplasmic beta- and gamma-actins.{http://www.cbil.upenn.edu/MTIR/gammaActin_E-toc.html}

Immunogen :

Synthetic peptide corresponding to the amino terminus of gamma actin {EEEIAALVIDNGSGGSG} conjugated to KLH.

Specificity :

Recognizes actin, gamma. Specificity for gamma actin determined by 2D gel electrophoresis.

Package Size :

100 μL

Uses :

Immunocytochemistry;Immunohistochemistry;Western Blotting

Storage :

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.