Oct-4 Antibody | EMD Millipore AB3209 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Oct-4 Antibody

catalog :

AB3209

quantity :

100 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500; fig 3	Moshfegh C, Aires L, Kisielow M, Vogel V. A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways. Sci Rep. 2016;6:25104 pubmed publisher
	Zhang S, Wang Y, Jia L, Wen X, Du Z, Wang C, et al. Profiling the long noncoding RNA interaction network in the regulatory elements of target genes by chromatin in situ reverse transcription sequencing. Genome Res. 2019;29:1521-1532 pubmed publisher

product information

Catalog Number :

AB3209

Subcategory :

Stem Cell Research

Product Name :

Anti-Oct-4 Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q01860

Host Name :

Rabbit

Antigen :

Oct 4

Conjugate :

Affinity Purified

Product Description :

Anti-Oct-4 Antibody

Cross Reactivity :

Human;Mouse

Background :

Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.

ALT Names :

POU5F1

Immunogen :

Synthetic peptide, amino acids 282-297of Oct4.

Specificity :

Recognizes Oct4 (POU5F1). The molecular weight of the protein is 38,601 Daltons.

Package Size :

100 μg

Uses :

Immunocytochemistry;Immunohistochemistry;Western Blotting

Storage :

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. The rehydrated antibody solutions can be stored undiluted at 2–8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2–8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.