Tubulin Antibody, Detyrosinated | EMD Millipore AB3201 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Tubulin Antibody, Detyrosinated

catalog :

AB3201

quantity :

50 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section

citations: 20

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 3c	Even A, Morelli G, Broix L, Scaramuzzino C, Turchetto S, Gladwyn Ng I, et al. ATAT1-enriched vesicles promote microtubule acetylation via axonal transport. Sci Adv. 2019;5:eaax2705 pubmed publisher
immunocytochemistry; human; loading ...; fig 2c	Wang R, Yu R, Zhu C, Lin H, Lu X, Wang H. Tubulin detyrosination promotes human trophoblast syncytium formation. J Mol Cell Biol. 2019;: pubmed publisher
immunocytochemistry; human; 1:200; loading ...; fig 1b	Han S, Miyoshi K, Shikada S, Amano G, Wang Y, Yoshimura T, et al. TULP3 is required for localization of membrane-associated proteins ARL13B and INPP5E to primary cilia. Biochem Biophys Res Commun. 2018;: pubmed publisher
western blot; human; loading ...; fig s6a	Wei J, Xu H, Meng W. Noncentrosomal microtubules regulate autophagosome transport through CAMSAP2-EB1 cross-talk. FEBS Lett. 2017;591:2379-2393 pubmed publisher
immunocytochemistry; human; 1:500; loading ...; fig 3 immunocytochemistry; mouse; 1:500; loading ...; fig 3	Hua K, Ferland R. Fixation methods can differentially affect ciliary protein immunolabeling. Cilia. 2017;6:5 pubmed publisher
western blot; mouse; 1 ug/ml; fig 5	Stritt S, Nurden P, Favier R, Favier M, Ferioli S, Gotru S, et al. Defects in TRPM7 channel function deregulate thrombopoiesis through altered cellular Mg(2+) homeostasis and cytoskeletal architecture. Nat Commun. 2016;7:11097 pubmed publisher
immunocytochemistry; mouse; 1:500; fig s5	Li C, Jensen V, Park K, Kennedy J, Garcia Gonzalo F, Romani M, et al. MKS5 and CEP290 Dependent Assembly Pathway of the Ciliary Transition Zone. PLoS Biol. 2016;14:e1002416 pubmed publisher
immunocytochemistry; human; 1:250; fig 4	Hori A, Barnouin K, Snijders A, Toda T. A non-canonical function of Plk4 in centriolar satellite integrity and ciliogenesis through PCM1 phosphorylation. EMBO Rep. 2016;17:326-37 pubmed publisher
	Nakagawa N, Plestant C, Yabuno Nakagawa K, Li J, Lee J, Huang C, et al. Memo1-Mediated Tiling of Radial Glial Cells Facilitates Cerebral Cortical Development. Neuron. 2019;: pubmed publisher
	Farías G, Fréal A, Tortosa E, Stucchi R, Pan X, Portegies S, et al. Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity. Neuron. 2019;102:184-201.e8 pubmed publisher
	Wang L, Buckley A, Spurney R. Regulation of cofilin phosphorylation in glomerular podocytes by testis specific kinase 1 (TESK1). Sci Rep. 2018;8:12286 pubmed publisher
	Latremoliere A, Cheng L, DeLisle M, Wu C, Chew S, Hutchinson E, et al. Neuronal-Specific TUBB3 Is Not Required for Normal Neuronal Function but Is Essential for Timely Axon Regeneration. Cell Rep. 2018;24:1865-1879.e9 pubmed publisher
	Qiang L, Sun X, Austin T, Muralidharan H, Jean D, Liu M, et al. Tau Does Not Stabilize Axonal Microtubules but Rather Enables Them to Have Long Labile Domains. Curr Biol. 2018;28:2181-2189.e4 pubmed publisher
	Vion A, Alt S, Klaus Bergmann A, Szymborska A, Zheng T, Perovic T, et al. Primary cilia sensitize endothelial cells to BMP and prevent excessive vascular regression. J Cell Biol. 2018;217:1651-1665 pubmed publisher
	Delaney M, Malikov V, Chai Q, Zhao G, Naghavi M. Distinct functions of diaphanous-related formins regulate HIV-1 uncoating and transport. Proc Natl Acad Sci U S A. 2017;114:E6932-E6941 pubmed publisher
	Ayloo S, Guedes Dias P, Ghiretti A, Holzbaur E. Dynein efficiently navigates the dendritic cytoskeleton to drive the retrograde trafficking of BDNF/TrkB signaling endosomes. Mol Biol Cell. 2017;28:2543-2554 pubmed publisher
	Loukil A, Tormanen K, SÃ¼tterlin C. The daughter centriole controls ciliogenesis by regulating Neurl-4 localization at the centrosome. J Cell Biol. 2017;216:1287-1300 pubmed publisher
	Erdogan B, Cammarata G, Lee E, Pratt B, Francl A, Rutherford E, et al. The microtubule plus-end-tracking protein TACC3 promotes persistent axon outgrowth and mediates responses to axon guidance signals during development. Neural Dev. 2017;12:3 pubmed publisher
	Schober T, Magg T, Laschinger M, Rohlfs M, Linhares N, Puchalka J, et al. A human immunodeficiency syndrome caused by mutations in CARMIL2. Nat Commun. 2017;8:14209 pubmed publisher
	Castellano E, Molina Arcas M, Krygowska A, East P, Warne P, Nicol A, et al. RAS signalling through PI3-Kinase controls cell migration via modulation of Reelin expression. Nat Commun. 2016;7:11245 pubmed publisher

product information

Catalog Number :

AB3201

Subcategory :

Cell Structure

Product Name :

Anti-Tubulin Antibody, Detyrosinated

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q13509

Host Name :

Rabbit

Antigen :

Tubulin

Conjugate :

Affinity Purified

Product Description :

Anti-Tubulin Antibody, Detyrosinated

Cross Reactivity :

Human;Mouse;Rat;Mammals;Green Plants;Sea Urchin

Background :

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

ALT Names :

Glu Tubulin

Immunogen :

Peptide corresponding to the C-terminal of Glu tubulin.

Specificity :

The antibody specifically recognizes the detyrosinated form (Glu tubulin) of the tubulin alpha chain (Bianchi, M., 2006; Goulimari, P., 2005).

Package Size :

50 µg

Uses :

Immunocytochemistry;Immunohistochemistry (Paraffin);Western Blotting

Storage :

Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.